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Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome

Sugarcane contributes 80% of global sugar production and to bioethanol generation for the bioenergy industry. Its productivity is threatened by drought that can cause up to 60% yield loss. This study used RNA-Seq to gain a better understanding of the underlying mechanism by which drought-tolerant su...

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Autores principales: Nawae, Wanapinun, Shearman, Jeremy R., Tangphatsornruang, Sithichoke, Punpee, Prapat, Yoocha, Thippawan, Sangsrakru, Duangjai, Naktang, Chaiwat, Sonthirod, Chutima, Wirojsirasak, Warodom, Ukoskit, Kittipat, Sriroth, Klanarong, Klomsa-ard, Peeraya, Pootakham, Wirulda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676377/
https://www.ncbi.nlm.nih.gov/pubmed/33240580
http://dx.doi.org/10.7717/peerj.9608
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author Nawae, Wanapinun
Shearman, Jeremy R.
Tangphatsornruang, Sithichoke
Punpee, Prapat
Yoocha, Thippawan
Sangsrakru, Duangjai
Naktang, Chaiwat
Sonthirod, Chutima
Wirojsirasak, Warodom
Ukoskit, Kittipat
Sriroth, Klanarong
Klomsa-ard, Peeraya
Pootakham, Wirulda
author_facet Nawae, Wanapinun
Shearman, Jeremy R.
Tangphatsornruang, Sithichoke
Punpee, Prapat
Yoocha, Thippawan
Sangsrakru, Duangjai
Naktang, Chaiwat
Sonthirod, Chutima
Wirojsirasak, Warodom
Ukoskit, Kittipat
Sriroth, Klanarong
Klomsa-ard, Peeraya
Pootakham, Wirulda
author_sort Nawae, Wanapinun
collection PubMed
description Sugarcane contributes 80% of global sugar production and to bioethanol generation for the bioenergy industry. Its productivity is threatened by drought that can cause up to 60% yield loss. This study used RNA-Seq to gain a better understanding of the underlying mechanism by which drought-tolerant sugarcane copes with water stress. We compared gene expression in KPS01-12 (drought-tolerant genotype) and UT12 (drought-sensitive genotype) that have significantly different yield loss rates under drought conditions. We treated KPS01-12 and UT12 with mild and moderate water stress and found differentially expressed genes in various biological processes. KPS01-12 had higher expression of genes that were involved in water retention, antioxidant secondary metabolite biosynthesis, and oxidative and osmotic stress response than UT12. In contrast, the sensitive genotype had more down-regulated genes that were involved in photosynthesis, carbon fixation and Calvin cycle than the tolerant genotype. Our obtained expression profiles suggest that the tolerant sugarcane has a more effective genetic response than the sensitive genotype at the initiation of drought stress. The knowledge gained from this study may be applied in breeding programs to improve sugarcane production in drought conditions.
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spelling pubmed-76763772020-11-24 Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome Nawae, Wanapinun Shearman, Jeremy R. Tangphatsornruang, Sithichoke Punpee, Prapat Yoocha, Thippawan Sangsrakru, Duangjai Naktang, Chaiwat Sonthirod, Chutima Wirojsirasak, Warodom Ukoskit, Kittipat Sriroth, Klanarong Klomsa-ard, Peeraya Pootakham, Wirulda PeerJ Bioinformatics Sugarcane contributes 80% of global sugar production and to bioethanol generation for the bioenergy industry. Its productivity is threatened by drought that can cause up to 60% yield loss. This study used RNA-Seq to gain a better understanding of the underlying mechanism by which drought-tolerant sugarcane copes with water stress. We compared gene expression in KPS01-12 (drought-tolerant genotype) and UT12 (drought-sensitive genotype) that have significantly different yield loss rates under drought conditions. We treated KPS01-12 and UT12 with mild and moderate water stress and found differentially expressed genes in various biological processes. KPS01-12 had higher expression of genes that were involved in water retention, antioxidant secondary metabolite biosynthesis, and oxidative and osmotic stress response than UT12. In contrast, the sensitive genotype had more down-regulated genes that were involved in photosynthesis, carbon fixation and Calvin cycle than the tolerant genotype. Our obtained expression profiles suggest that the tolerant sugarcane has a more effective genetic response than the sensitive genotype at the initiation of drought stress. The knowledge gained from this study may be applied in breeding programs to improve sugarcane production in drought conditions. PeerJ Inc. 2020-07-28 /pmc/articles/PMC7676377/ /pubmed/33240580 http://dx.doi.org/10.7717/peerj.9608 Text en © 2020 Nawae et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Nawae, Wanapinun
Shearman, Jeremy R.
Tangphatsornruang, Sithichoke
Punpee, Prapat
Yoocha, Thippawan
Sangsrakru, Duangjai
Naktang, Chaiwat
Sonthirod, Chutima
Wirojsirasak, Warodom
Ukoskit, Kittipat
Sriroth, Klanarong
Klomsa-ard, Peeraya
Pootakham, Wirulda
Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome
title Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome
title_full Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome
title_fullStr Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome
title_full_unstemmed Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome
title_short Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome
title_sort differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676377/
https://www.ncbi.nlm.nih.gov/pubmed/33240580
http://dx.doi.org/10.7717/peerj.9608
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