Cargando…

Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model

PURPOSE: The treatment strategy is different for acute traumatic peripheral nerve injury and acute compressive neuropathy. This study aimed to compare magnetic resonance imaging (MRI) features of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model. MATERIALS AND M...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Bo Ra, Ha, Dong-Ho, Kim, Jong Kuk, Kim, Young Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676645/
https://www.ncbi.nlm.nih.gov/pubmed/33211695
http://dx.doi.org/10.1371/journal.pone.0240911
_version_ 1783611812895260672
author Kim, Bo Ra
Ha, Dong-Ho
Kim, Jong Kuk
Kim, Young Hee
author_facet Kim, Bo Ra
Ha, Dong-Ho
Kim, Jong Kuk
Kim, Young Hee
author_sort Kim, Bo Ra
collection PubMed
description PURPOSE: The treatment strategy is different for acute traumatic peripheral nerve injury and acute compressive neuropathy. This study aimed to compare magnetic resonance imaging (MRI) features of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model. MATERIALS AND METHODS: Twenty female Sprague-Dawley rats were divided into two groups. In the crush injury group (n = 10), the unilateral sciatic nerve was crushed using forceps to represent acute traumatic peripheral nerve injury. In the compression injury group (n = 10), the unilateral sciatic nerve was ligated using silk to represent acute compressive neuropathy. The MRI of eight rats from each group were acquired on postoperative days 3 and 10. Fat-suppressed T2-weighted images were acquired. Changes in the injured nerve were divided into three grades. A Fisher’s exact test was used to compare the changes in the nerves of the two groups. Histological staining and a western blot analysis were performed on one rat in each group on day 3. Neurofilament, myelin basic protein (MBP), and p75(NTR) staining were performed. Expression of neurofilament, MBP, p75(NTR), and c-jun was evaluated by western blot analysis. RESULTS: MR neurography revealed substantial nerve changes in the compression injury group compared with the crush injury group at two-time points (p = 0.001 on day 3, p = 0.026 on day 10). The histopathological analysis indicated the destruction of the axon and myelin, mainly at the injury site and the distal portion of the injury in the crush injury group. It was prominent in the proximal portion, the injury site, and the distal portion of the injury in the compression injury group. The degree of axonal and myelin destruction was more pronounced in the compression injury group than in the crush injury group. CONCLUSION: MR neurography showed prominent and long-segmental changes associated with the injured nerve in acute compressive neuropathy compared with acute traumatic peripheral nerve injury.
format Online
Article
Text
id pubmed-7676645
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-76766452020-12-02 Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model Kim, Bo Ra Ha, Dong-Ho Kim, Jong Kuk Kim, Young Hee PLoS One Research Article PURPOSE: The treatment strategy is different for acute traumatic peripheral nerve injury and acute compressive neuropathy. This study aimed to compare magnetic resonance imaging (MRI) features of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model. MATERIALS AND METHODS: Twenty female Sprague-Dawley rats were divided into two groups. In the crush injury group (n = 10), the unilateral sciatic nerve was crushed using forceps to represent acute traumatic peripheral nerve injury. In the compression injury group (n = 10), the unilateral sciatic nerve was ligated using silk to represent acute compressive neuropathy. The MRI of eight rats from each group were acquired on postoperative days 3 and 10. Fat-suppressed T2-weighted images were acquired. Changes in the injured nerve were divided into three grades. A Fisher’s exact test was used to compare the changes in the nerves of the two groups. Histological staining and a western blot analysis were performed on one rat in each group on day 3. Neurofilament, myelin basic protein (MBP), and p75(NTR) staining were performed. Expression of neurofilament, MBP, p75(NTR), and c-jun was evaluated by western blot analysis. RESULTS: MR neurography revealed substantial nerve changes in the compression injury group compared with the crush injury group at two-time points (p = 0.001 on day 3, p = 0.026 on day 10). The histopathological analysis indicated the destruction of the axon and myelin, mainly at the injury site and the distal portion of the injury in the crush injury group. It was prominent in the proximal portion, the injury site, and the distal portion of the injury in the compression injury group. The degree of axonal and myelin destruction was more pronounced in the compression injury group than in the crush injury group. CONCLUSION: MR neurography showed prominent and long-segmental changes associated with the injured nerve in acute compressive neuropathy compared with acute traumatic peripheral nerve injury. Public Library of Science 2020-11-19 /pmc/articles/PMC7676645/ /pubmed/33211695 http://dx.doi.org/10.1371/journal.pone.0240911 Text en © 2020 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kim, Bo Ra
Ha, Dong-Ho
Kim, Jong Kuk
Kim, Young Hee
Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model
title Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model
title_full Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model
title_fullStr Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model
title_full_unstemmed Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model
title_short Comparison of MR findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model
title_sort comparison of mr findings of acute traumatic peripheral nerve injury and acute compressive neuropathy in a rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676645/
https://www.ncbi.nlm.nih.gov/pubmed/33211695
http://dx.doi.org/10.1371/journal.pone.0240911
work_keys_str_mv AT kimbora comparisonofmrfindingsofacutetraumaticperipheralnerveinjuryandacutecompressiveneuropathyinaratmodel
AT hadongho comparisonofmrfindingsofacutetraumaticperipheralnerveinjuryandacutecompressiveneuropathyinaratmodel
AT kimjongkuk comparisonofmrfindingsofacutetraumaticperipheralnerveinjuryandacutecompressiveneuropathyinaratmodel
AT kimyounghee comparisonofmrfindingsofacutetraumaticperipheralnerveinjuryandacutecompressiveneuropathyinaratmodel