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Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic
BACKGROUND: Antibody diagnostics play an important role in disease detection and can potentially aid in monitoring of the immune responses to see if an individual has developed immunity. Developing high throughput diagnostics which does not involve handling of infectious material becomes imperative...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676701/ https://www.ncbi.nlm.nih.gov/pubmed/33211781 http://dx.doi.org/10.1371/journal.pone.0242655 |
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author | Krishnamurthy, Hari Krishnan Jayaraman, Vasanth Krishna, Karthik Rajasekaran, Karenah E. Wang, Tianhao Bei, Kang Rajasekaran, John J. Yaskin, Inna Rai, Alex J. Choung, Rok Seon Murray, Joseph A. |
author_facet | Krishnamurthy, Hari Krishnan Jayaraman, Vasanth Krishna, Karthik Rajasekaran, Karenah E. Wang, Tianhao Bei, Kang Rajasekaran, John J. Yaskin, Inna Rai, Alex J. Choung, Rok Seon Murray, Joseph A. |
author_sort | Krishnamurthy, Hari Krishnan |
collection | PubMed |
description | BACKGROUND: Antibody diagnostics play an important role in disease detection and can potentially aid in monitoring of the immune responses to see if an individual has developed immunity. Developing high throughput diagnostics which does not involve handling of infectious material becomes imperative in the case of pandemics such as the recent outbreak of SARS-CoV2. METHODS: A protein microarray technology was used to detect the plurality of antibody response to four novel antigens namely S1 glycoprotein, Receptor binding domain (RBD), S2 glycoprotein and Nucleoprotein of the novel coronavirus named SARS-CoV2 using serum samples. A DBS card was additionally used to compare its performance with a venipuncture-based serum separator tube (SST) draw. RESULTS: The three main subclasses of antibodies IgM, IgA and IgG were analyzed to see the variations in immune responses in the affected population and compared to their microbial RT-PCR based NP swab results. The clinical sensitivity and specificity were determined to be 99.67% and 99.77%. In the matrix comparison study, which would enable patients to test without risk of transmitting the virus, DBS (Dried Blood Spot) matched with higher than 98% accuracy to a venipuncture-based SST collection. CONCLUSION: Multiplex testing enables higher sensitivity and specificity which is essential while establishing exposure on a population scale. This flexible platform along with a discrete collection methodology would be crucial and broadly useful to scale up testing in current and future pandemics. Minimum sample volume that can be collected using DBS cards can be processed in this multiplex pillar plate format enabling the capacity to provide the reliability of high throughput analyzers while having the ease of collection similar to rapid tests. |
format | Online Article Text |
id | pubmed-7676701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76767012020-12-02 Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic Krishnamurthy, Hari Krishnan Jayaraman, Vasanth Krishna, Karthik Rajasekaran, Karenah E. Wang, Tianhao Bei, Kang Rajasekaran, John J. Yaskin, Inna Rai, Alex J. Choung, Rok Seon Murray, Joseph A. PLoS One Research Article BACKGROUND: Antibody diagnostics play an important role in disease detection and can potentially aid in monitoring of the immune responses to see if an individual has developed immunity. Developing high throughput diagnostics which does not involve handling of infectious material becomes imperative in the case of pandemics such as the recent outbreak of SARS-CoV2. METHODS: A protein microarray technology was used to detect the plurality of antibody response to four novel antigens namely S1 glycoprotein, Receptor binding domain (RBD), S2 glycoprotein and Nucleoprotein of the novel coronavirus named SARS-CoV2 using serum samples. A DBS card was additionally used to compare its performance with a venipuncture-based serum separator tube (SST) draw. RESULTS: The three main subclasses of antibodies IgM, IgA and IgG were analyzed to see the variations in immune responses in the affected population and compared to their microbial RT-PCR based NP swab results. The clinical sensitivity and specificity were determined to be 99.67% and 99.77%. In the matrix comparison study, which would enable patients to test without risk of transmitting the virus, DBS (Dried Blood Spot) matched with higher than 98% accuracy to a venipuncture-based SST collection. CONCLUSION: Multiplex testing enables higher sensitivity and specificity which is essential while establishing exposure on a population scale. This flexible platform along with a discrete collection methodology would be crucial and broadly useful to scale up testing in current and future pandemics. Minimum sample volume that can be collected using DBS cards can be processed in this multiplex pillar plate format enabling the capacity to provide the reliability of high throughput analyzers while having the ease of collection similar to rapid tests. Public Library of Science 2020-11-19 /pmc/articles/PMC7676701/ /pubmed/33211781 http://dx.doi.org/10.1371/journal.pone.0242655 Text en © 2020 Krishnamurthy et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Krishnamurthy, Hari Krishnan Jayaraman, Vasanth Krishna, Karthik Rajasekaran, Karenah E. Wang, Tianhao Bei, Kang Rajasekaran, John J. Yaskin, Inna Rai, Alex J. Choung, Rok Seon Murray, Joseph A. Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic |
title | Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic |
title_full | Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic |
title_fullStr | Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic |
title_full_unstemmed | Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic |
title_short | Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic |
title_sort | antibody profiling and prevalence in us patients during the sars-cov2 pandemic |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676701/ https://www.ncbi.nlm.nih.gov/pubmed/33211781 http://dx.doi.org/10.1371/journal.pone.0242655 |
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