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Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata

Endophytic fungi play an important role in plant growth. The composition and structure of endophytes vary in different plant tissues, which are specific habitats for endophyte colonization. To analyze the diversity and structural composition of endophytic fungi from toothed clubmoss (Huperzia serrat...

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Autores principales: Fan, Shipeng, Miao, Liyun, Li, Haodong, Lin, Aihua, Song, Fajun, Zhang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676737/
https://www.ncbi.nlm.nih.gov/pubmed/33211760
http://dx.doi.org/10.1371/journal.pone.0242258
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author Fan, Shipeng
Miao, Liyun
Li, Haodong
Lin, Aihua
Song, Fajun
Zhang, Peng
author_facet Fan, Shipeng
Miao, Liyun
Li, Haodong
Lin, Aihua
Song, Fajun
Zhang, Peng
author_sort Fan, Shipeng
collection PubMed
description Endophytic fungi play an important role in plant growth. The composition and structure of endophytes vary in different plant tissues, which are specific habitats for endophyte colonization. To analyze the diversity and structural composition of endophytic fungi from toothed clubmoss (Huperzia serrata) that was artificially cultivated for 3 years, we investigated endophytic fungi from the roots, stems and leaves using comparative sequence analysis of the ITS2 region of the fungal rRNA genes sequenced with high-throughput sequencing technology. Seven fungal phyla were identified, and fungal diversity and structure varied across different tissues, with the most distinctive community features found in the roots. A total of 555 operational taxonomic units (OTUs) were detected, and 198 were common to all samples, and 43, 16, 16 OTUs were unique to the root, stem, leaf samples, respectively. Taxonomic classification showed that Ascomycota and Basidiomycota were dominant phyla, and Cladosporium, Oidiodendron, Phyllosticta, Sebacina and Ilyonectria were dominant genera. The relative abundance heat map at the genus level suggested that H. serrata had characteristic endophytic fungal microbiomes. Line discriminant analysis effect size analysis and principal coordinate analysis demonstrated that fungal communities were tissue-type and tissue-site specific. Overall, our study provides new insights into the complex composition of endophytic fungi in H. serrata.
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spelling pubmed-76767372020-12-02 Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata Fan, Shipeng Miao, Liyun Li, Haodong Lin, Aihua Song, Fajun Zhang, Peng PLoS One Research Article Endophytic fungi play an important role in plant growth. The composition and structure of endophytes vary in different plant tissues, which are specific habitats for endophyte colonization. To analyze the diversity and structural composition of endophytic fungi from toothed clubmoss (Huperzia serrata) that was artificially cultivated for 3 years, we investigated endophytic fungi from the roots, stems and leaves using comparative sequence analysis of the ITS2 region of the fungal rRNA genes sequenced with high-throughput sequencing technology. Seven fungal phyla were identified, and fungal diversity and structure varied across different tissues, with the most distinctive community features found in the roots. A total of 555 operational taxonomic units (OTUs) were detected, and 198 were common to all samples, and 43, 16, 16 OTUs were unique to the root, stem, leaf samples, respectively. Taxonomic classification showed that Ascomycota and Basidiomycota were dominant phyla, and Cladosporium, Oidiodendron, Phyllosticta, Sebacina and Ilyonectria were dominant genera. The relative abundance heat map at the genus level suggested that H. serrata had characteristic endophytic fungal microbiomes. Line discriminant analysis effect size analysis and principal coordinate analysis demonstrated that fungal communities were tissue-type and tissue-site specific. Overall, our study provides new insights into the complex composition of endophytic fungi in H. serrata. Public Library of Science 2020-11-19 /pmc/articles/PMC7676737/ /pubmed/33211760 http://dx.doi.org/10.1371/journal.pone.0242258 Text en © 2020 Fan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fan, Shipeng
Miao, Liyun
Li, Haodong
Lin, Aihua
Song, Fajun
Zhang, Peng
Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata
title Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata
title_full Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata
title_fullStr Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata
title_full_unstemmed Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata
title_short Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata
title_sort illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated huperzia serrata
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676737/
https://www.ncbi.nlm.nih.gov/pubmed/33211760
http://dx.doi.org/10.1371/journal.pone.0242258
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