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Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster
Ribosomal RNAs (rRNAs) are essential components of the ribosome and are among the most abundant macromolecules in the cell. To ensure high rRNA level, eukaryotic genomes contain dozens to hundreds of rDNA genes, however, only a fraction of the rRNA genes seems to be active, while others are transcri...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676866/ https://www.ncbi.nlm.nih.gov/pubmed/33164748 http://dx.doi.org/10.7554/eLife.52416 |
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author | Luo, Yicheng Fefelova, Elena Ninova, Maria Chen, Yung-Chia Ariel Aravin, Alexei A |
author_facet | Luo, Yicheng Fefelova, Elena Ninova, Maria Chen, Yung-Chia Ariel Aravin, Alexei A |
author_sort | Luo, Yicheng |
collection | PubMed |
description | Ribosomal RNAs (rRNAs) are essential components of the ribosome and are among the most abundant macromolecules in the cell. To ensure high rRNA level, eukaryotic genomes contain dozens to hundreds of rDNA genes, however, only a fraction of the rRNA genes seems to be active, while others are transcriptionally silent. We found that individual rDNA genes have high level of cell-to-cell heterogeneity in their expression in Drosophila melanogaster. Insertion of heterologous sequences into rDNA leads to repression associated with reduced expression in individual cells and decreased number of cells expressing rDNA with insertions. We found that SUMO (Small Ubiquitin-like Modifier) and SUMO ligase Ubc9 are required for efficient repression of interrupted rDNA units and variable expression of intact rDNA. Disruption of the SUMO pathway abolishes discrimination of interrupted and intact rDNAs and removes cell-to-cell heterogeneity leading to uniformly high expression of individual rDNA in single cells. Our results suggest that the SUMO pathway is responsible for both repression of interrupted units and control of intact rDNA expression. |
format | Online Article Text |
id | pubmed-7676866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-76768662020-11-23 Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster Luo, Yicheng Fefelova, Elena Ninova, Maria Chen, Yung-Chia Ariel Aravin, Alexei A eLife Chromosomes and Gene Expression Ribosomal RNAs (rRNAs) are essential components of the ribosome and are among the most abundant macromolecules in the cell. To ensure high rRNA level, eukaryotic genomes contain dozens to hundreds of rDNA genes, however, only a fraction of the rRNA genes seems to be active, while others are transcriptionally silent. We found that individual rDNA genes have high level of cell-to-cell heterogeneity in their expression in Drosophila melanogaster. Insertion of heterologous sequences into rDNA leads to repression associated with reduced expression in individual cells and decreased number of cells expressing rDNA with insertions. We found that SUMO (Small Ubiquitin-like Modifier) and SUMO ligase Ubc9 are required for efficient repression of interrupted rDNA units and variable expression of intact rDNA. Disruption of the SUMO pathway abolishes discrimination of interrupted and intact rDNAs and removes cell-to-cell heterogeneity leading to uniformly high expression of individual rDNA in single cells. Our results suggest that the SUMO pathway is responsible for both repression of interrupted units and control of intact rDNA expression. eLife Sciences Publications, Ltd 2020-11-09 /pmc/articles/PMC7676866/ /pubmed/33164748 http://dx.doi.org/10.7554/eLife.52416 Text en © 2020, Luo et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Chromosomes and Gene Expression Luo, Yicheng Fefelova, Elena Ninova, Maria Chen, Yung-Chia Ariel Aravin, Alexei A Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster |
title | Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster |
title_full | Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster |
title_fullStr | Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster |
title_full_unstemmed | Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster |
title_short | Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster |
title_sort | repression of interrupted and intact rdna by the sumo pathway in drosophila melanogaster |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676866/ https://www.ncbi.nlm.nih.gov/pubmed/33164748 http://dx.doi.org/10.7554/eLife.52416 |
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