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Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice

Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH). Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccha...

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Autores principales: Watahiki, Takahisa, Okada, Kosuke, Warabi, Eiji, Nagaoka, Tsugumi, Suzuki, Hideo, Ishige, Kazunori, Yanagawa, Toru, Takahashi, Satoru, Mizokami, Yuji, Tokushige, Katsutoshi, Ariizumi, Shun-ichi, Yamamoto, Masakazu, Shoda, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677087/
https://www.ncbi.nlm.nih.gov/pubmed/32493884
http://dx.doi.org/10.1538/expanim.20-0028
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author Watahiki, Takahisa
Okada, Kosuke
Warabi, Eiji
Nagaoka, Tsugumi
Suzuki, Hideo
Ishige, Kazunori
Yanagawa, Toru
Takahashi, Satoru
Mizokami, Yuji
Tokushige, Katsutoshi
Ariizumi, Shun-ichi
Yamamoto, Masakazu
Shoda, Junichi
author_facet Watahiki, Takahisa
Okada, Kosuke
Warabi, Eiji
Nagaoka, Tsugumi
Suzuki, Hideo
Ishige, Kazunori
Yanagawa, Toru
Takahashi, Satoru
Mizokami, Yuji
Tokushige, Katsutoshi
Ariizumi, Shun-ichi
Yamamoto, Masakazu
Shoda, Junichi
author_sort Watahiki, Takahisa
collection PubMed
description Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH). Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells. However, the pathogenetic phenotype of steatohepatitis in female DKO mice remains unknown. Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences. Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis. Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period). Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice. Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice. Gender differences were observed for the development of steatohepatitis in DKO mice. Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
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spelling pubmed-76770872020-11-24 Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice Watahiki, Takahisa Okada, Kosuke Warabi, Eiji Nagaoka, Tsugumi Suzuki, Hideo Ishige, Kazunori Yanagawa, Toru Takahashi, Satoru Mizokami, Yuji Tokushige, Katsutoshi Ariizumi, Shun-ichi Yamamoto, Masakazu Shoda, Junichi Exp Anim Original Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH). Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells. However, the pathogenetic phenotype of steatohepatitis in female DKO mice remains unknown. Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences. Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis. Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period). Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice. Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice. Gender differences were observed for the development of steatohepatitis in DKO mice. Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice. Japanese Association for Laboratory Animal Science 2020-06-03 2020 /pmc/articles/PMC7677087/ /pubmed/32493884 http://dx.doi.org/10.1538/expanim.20-0028 Text en ©2020 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Watahiki, Takahisa
Okada, Kosuke
Warabi, Eiji
Nagaoka, Tsugumi
Suzuki, Hideo
Ishige, Kazunori
Yanagawa, Toru
Takahashi, Satoru
Mizokami, Yuji
Tokushige, Katsutoshi
Ariizumi, Shun-ichi
Yamamoto, Masakazu
Shoda, Junichi
Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice
title Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice
title_full Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice
title_fullStr Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice
title_full_unstemmed Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice
title_short Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice
title_sort gender difference in development of steatohepatitis in p62/sqstm1 and nrf2 double-knockout mice
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677087/
https://www.ncbi.nlm.nih.gov/pubmed/32493884
http://dx.doi.org/10.1538/expanim.20-0028
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