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Simple transport and cryopreservation of cold-stored mouse embryos
The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transfe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677088/ https://www.ncbi.nlm.nih.gov/pubmed/32554941 http://dx.doi.org/10.1538/expanim.20-0042 |
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author | Mukunoki, Ayumi Takeo, Toru Nakao, Satohiro Tamura, Kana Horikoshi, Yuka Nakagata, Naomi |
author_facet | Mukunoki, Ayumi Takeo, Toru Nakao, Satohiro Tamura, Kana Horikoshi, Yuka Nakagata, Naomi |
author_sort | Mukunoki, Ayumi |
collection | PubMed |
description | The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice. |
format | Online Article Text |
id | pubmed-7677088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76770882020-11-24 Simple transport and cryopreservation of cold-stored mouse embryos Mukunoki, Ayumi Takeo, Toru Nakao, Satohiro Tamura, Kana Horikoshi, Yuka Nakagata, Naomi Exp Anim Original The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice. Japanese Association for Laboratory Animal Science 2020-06-17 2020 /pmc/articles/PMC7677088/ /pubmed/32554941 http://dx.doi.org/10.1538/expanim.20-0042 Text en ©2020 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Mukunoki, Ayumi Takeo, Toru Nakao, Satohiro Tamura, Kana Horikoshi, Yuka Nakagata, Naomi Simple transport and cryopreservation of cold-stored mouse embryos |
title | Simple transport and cryopreservation of cold-stored mouse
embryos |
title_full | Simple transport and cryopreservation of cold-stored mouse
embryos |
title_fullStr | Simple transport and cryopreservation of cold-stored mouse
embryos |
title_full_unstemmed | Simple transport and cryopreservation of cold-stored mouse
embryos |
title_short | Simple transport and cryopreservation of cold-stored mouse
embryos |
title_sort | simple transport and cryopreservation of cold-stored mouse
embryos |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677088/ https://www.ncbi.nlm.nih.gov/pubmed/32554941 http://dx.doi.org/10.1538/expanim.20-0042 |
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