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Simple transport and cryopreservation of cold-stored mouse embryos

The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transfe...

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Autores principales: Mukunoki, Ayumi, Takeo, Toru, Nakao, Satohiro, Tamura, Kana, Horikoshi, Yuka, Nakagata, Naomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677088/
https://www.ncbi.nlm.nih.gov/pubmed/32554941
http://dx.doi.org/10.1538/expanim.20-0042
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author Mukunoki, Ayumi
Takeo, Toru
Nakao, Satohiro
Tamura, Kana
Horikoshi, Yuka
Nakagata, Naomi
author_facet Mukunoki, Ayumi
Takeo, Toru
Nakao, Satohiro
Tamura, Kana
Horikoshi, Yuka
Nakagata, Naomi
author_sort Mukunoki, Ayumi
collection PubMed
description The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.
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spelling pubmed-76770882020-11-24 Simple transport and cryopreservation of cold-stored mouse embryos Mukunoki, Ayumi Takeo, Toru Nakao, Satohiro Tamura, Kana Horikoshi, Yuka Nakagata, Naomi Exp Anim Original The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice. Japanese Association for Laboratory Animal Science 2020-06-17 2020 /pmc/articles/PMC7677088/ /pubmed/32554941 http://dx.doi.org/10.1538/expanim.20-0042 Text en ©2020 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Mukunoki, Ayumi
Takeo, Toru
Nakao, Satohiro
Tamura, Kana
Horikoshi, Yuka
Nakagata, Naomi
Simple transport and cryopreservation of cold-stored mouse embryos
title Simple transport and cryopreservation of cold-stored mouse embryos
title_full Simple transport and cryopreservation of cold-stored mouse embryos
title_fullStr Simple transport and cryopreservation of cold-stored mouse embryos
title_full_unstemmed Simple transport and cryopreservation of cold-stored mouse embryos
title_short Simple transport and cryopreservation of cold-stored mouse embryos
title_sort simple transport and cryopreservation of cold-stored mouse embryos
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677088/
https://www.ncbi.nlm.nih.gov/pubmed/32554941
http://dx.doi.org/10.1538/expanim.20-0042
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