Low-Level Mouse DNA in Conditioned Medium Generates False Positive Cross-Species Contamination Results in Human Organoid Cultures

Cell line authentication is critical for preventing the use of mixed or misidentified cell lines in research. Current efforts include short tandem repeat (STR) analysis and PCR-based assays to detect mixed species cultures. Using PCR analysis with mouse-specific primers, we identified contaminating...

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Detalles Bibliográficos
Autores principales: Bohm, Margaret S., Dame, Michael K., Boyd, Joseph, Su, Kevin, Wu, Angeline, Attili, Durga, Chu, Vi, Colacino, Justin A., Spence, Jason R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677229/
https://www.ncbi.nlm.nih.gov/pubmed/33240885
http://dx.doi.org/10.3389/fcell.2020.587107

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