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The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty

PURPOSE: We detected the DNA of herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in donor corneas and assessed the clinical outcomes of recipients who received virus-positive grafts. METHOD: Al...

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Autores principales: Qu, Jing-hao, Peng, Rong-mei, Xiao, Ge-ge, Qu, Hong-qiang, Yu, Ting, Zhang, Shuang, Hong, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677267/
https://www.ncbi.nlm.nih.gov/pubmed/33097979
http://dx.doi.org/10.1007/s00417-020-04984-2
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author Qu, Jing-hao
Peng, Rong-mei
Xiao, Ge-ge
Qu, Hong-qiang
Yu, Ting
Zhang, Shuang
Hong, Jing
author_facet Qu, Jing-hao
Peng, Rong-mei
Xiao, Ge-ge
Qu, Hong-qiang
Yu, Ting
Zhang, Shuang
Hong, Jing
author_sort Qu, Jing-hao
collection PubMed
description PURPOSE: We detected the DNA of herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in donor corneas and assessed the clinical outcomes of recipients who received virus-positive grafts. METHOD: All donor corneas were analyzed for the presence of HSV-1, HSV-2, VZV, CMV, and EBV by real-time PCR from April 2017 to July 2019. The medical records of the transplant patients who received virus-positive grafts were reviewed. RESULT: Twenty-three (2.44%) donor cornea buttons tested positive for herpesviridae DNA. The positivity rates of HSV-1, CMV, VZV, and EBV were 0.74%, 0.85%, 0.64%, and 0.21%, respectively. CONCLUSION: We suggest that the corneas from donors who had cancer, donors who were inpatients, and donors who had immunodeficiency or who were on immunosuppressive therapy should be tested for herpesviridae DNA before transplantation. Finally, HSV-1 can be transmitted from graft to recipient, but that CMV cannot be transmitted according to our observations. The donor corneas found to be HSV-1-positive have to be discarded and not used for keratoplasty. [Image: see text]
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spelling pubmed-76772672020-11-23 The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty Qu, Jing-hao Peng, Rong-mei Xiao, Ge-ge Qu, Hong-qiang Yu, Ting Zhang, Shuang Hong, Jing Graefes Arch Clin Exp Ophthalmol Cornea PURPOSE: We detected the DNA of herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in donor corneas and assessed the clinical outcomes of recipients who received virus-positive grafts. METHOD: All donor corneas were analyzed for the presence of HSV-1, HSV-2, VZV, CMV, and EBV by real-time PCR from April 2017 to July 2019. The medical records of the transplant patients who received virus-positive grafts were reviewed. RESULT: Twenty-three (2.44%) donor cornea buttons tested positive for herpesviridae DNA. The positivity rates of HSV-1, CMV, VZV, and EBV were 0.74%, 0.85%, 0.64%, and 0.21%, respectively. CONCLUSION: We suggest that the corneas from donors who had cancer, donors who were inpatients, and donors who had immunodeficiency or who were on immunosuppressive therapy should be tested for herpesviridae DNA before transplantation. Finally, HSV-1 can be transmitted from graft to recipient, but that CMV cannot be transmitted according to our observations. The donor corneas found to be HSV-1-positive have to be discarded and not used for keratoplasty. [Image: see text] Springer Berlin Heidelberg 2020-10-24 2020 /pmc/articles/PMC7677267/ /pubmed/33097979 http://dx.doi.org/10.1007/s00417-020-04984-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Cornea
Qu, Jing-hao
Peng, Rong-mei
Xiao, Ge-ge
Qu, Hong-qiang
Yu, Ting
Zhang, Shuang
Hong, Jing
The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty
title The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty
title_full The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty
title_fullStr The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty
title_full_unstemmed The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty
title_short The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty
title_sort incidence and influence of the donor corneas positive for herpesviridae dna in keratoplasty
topic Cornea
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677267/
https://www.ncbi.nlm.nih.gov/pubmed/33097979
http://dx.doi.org/10.1007/s00417-020-04984-2
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