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Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion
Background: Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and precancerous oral lesion with high incidence. The current diagnostic method of OLP is very limited and metabolomics may provide a new approach for quantitative evaluation. Methods: The Ultra-Performance Liqui...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677577/ https://www.ncbi.nlm.nih.gov/pubmed/33240093 http://dx.doi.org/10.3389/fphar.2020.603899 |
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author | Wang, Xiao-Shuang Sun, Zhi Liu, Li-Wei Du, Qiu-Zheng Liu, Zhang-Suo Yang, Yan-Jie Xue, Peng Zhao, Hong-Yu |
author_facet | Wang, Xiao-Shuang Sun, Zhi Liu, Li-Wei Du, Qiu-Zheng Liu, Zhang-Suo Yang, Yan-Jie Xue, Peng Zhao, Hong-Yu |
author_sort | Wang, Xiao-Shuang |
collection | PubMed |
description | Background: Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and precancerous oral lesion with high incidence. The current diagnostic method of OLP is very limited and metabolomics may provide a new approach for quantitative evaluation. Methods: The Ultra-Performance Liquid Chromatography-Quadrupole/Orbitrap High Resolution Mass Spectrometry (UHPLC-Q-Orbitrap HRMS) was applied to analyze the change of metabolites in serum of patients with OLP. A total of 115 OLP patients and 124 healthy controls were assigned to either a training set (n = 160) or a test set (n = 79). The potential biomarkers and the change of serum metabolites were profiled and evaluated by multivariate analysis. Results: Totally, 23 differential metabolites were identified in the training set between OLP group and healthy group. Three prominent metabolites in receiver operating characteristic (ROC) were selected as a panel to distinguish OLP or healthy individuals in the test set, and the diagnostic accuracy was 86.1%. Conclusions: This study established a new method for the early detection of OLP by analyzing serum metabolomics using UHPLC-Q-Orbitrap HRMS, which will help in understanding the pathological processes of OLP and identifying precancerous lesions in oral cavity. |
format | Online Article Text |
id | pubmed-7677577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76775772020-11-24 Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion Wang, Xiao-Shuang Sun, Zhi Liu, Li-Wei Du, Qiu-Zheng Liu, Zhang-Suo Yang, Yan-Jie Xue, Peng Zhao, Hong-Yu Front Pharmacol Pharmacology Background: Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disorder and precancerous oral lesion with high incidence. The current diagnostic method of OLP is very limited and metabolomics may provide a new approach for quantitative evaluation. Methods: The Ultra-Performance Liquid Chromatography-Quadrupole/Orbitrap High Resolution Mass Spectrometry (UHPLC-Q-Orbitrap HRMS) was applied to analyze the change of metabolites in serum of patients with OLP. A total of 115 OLP patients and 124 healthy controls were assigned to either a training set (n = 160) or a test set (n = 79). The potential biomarkers and the change of serum metabolites were profiled and evaluated by multivariate analysis. Results: Totally, 23 differential metabolites were identified in the training set between OLP group and healthy group. Three prominent metabolites in receiver operating characteristic (ROC) were selected as a panel to distinguish OLP or healthy individuals in the test set, and the diagnostic accuracy was 86.1%. Conclusions: This study established a new method for the early detection of OLP by analyzing serum metabolomics using UHPLC-Q-Orbitrap HRMS, which will help in understanding the pathological processes of OLP and identifying precancerous lesions in oral cavity. Frontiers Media S.A. 2020-11-06 /pmc/articles/PMC7677577/ /pubmed/33240093 http://dx.doi.org/10.3389/fphar.2020.603899 Text en Copyright © 2020 Wang, Sun, Liu, Du, Liu, Yang, Xue and Zhao http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Wang, Xiao-Shuang Sun, Zhi Liu, Li-Wei Du, Qiu-Zheng Liu, Zhang-Suo Yang, Yan-Jie Xue, Peng Zhao, Hong-Yu Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion |
title | Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion |
title_full | Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion |
title_fullStr | Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion |
title_full_unstemmed | Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion |
title_short | Potential Metabolic Biomarkers for Early Detection of Oral Lichen Planus, a Precancerous Lesion |
title_sort | potential metabolic biomarkers for early detection of oral lichen planus, a precancerous lesion |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677577/ https://www.ncbi.nlm.nih.gov/pubmed/33240093 http://dx.doi.org/10.3389/fphar.2020.603899 |
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