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Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats

Resident and infiltrative macrophages play important roles in the development of pathological lesions. M1/M2 macrophage polarization with respective CD68 and CD163 expression remains unclear in chemically induced liver injury. This study was aimed at investigating the influence of macrophages on nor...

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Autores principales: Hada, Noa, Kuramochi, Mizuki, Izawa, Takeshi, Kuwamura, Mitsuru, Yamate, Jyoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Toxicologic Pathology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677630/
https://www.ncbi.nlm.nih.gov/pubmed/33239842
http://dx.doi.org/10.1293/tox.2020-0016
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author Hada, Noa
Kuramochi, Mizuki
Izawa, Takeshi
Kuwamura, Mitsuru
Yamate, Jyoji
author_facet Hada, Noa
Kuramochi, Mizuki
Izawa, Takeshi
Kuwamura, Mitsuru
Yamate, Jyoji
author_sort Hada, Noa
collection PubMed
description Resident and infiltrative macrophages play important roles in the development of pathological lesions. M1/M2 macrophage polarization with respective CD68 and CD163 expression remains unclear in chemically induced liver injury. This study was aimed at investigating the influence of macrophages on normal and chemically induced liver injury. For this, dexamethasone (DX), an immunosuppressive drug, was administered in normal rats and thioacetamide (TAA)-treated rats. Liver samples were collected and analyzed with immunohistochemical methods. Repeated injections of DX (0.5 or 1.0 mg/kg BW) for 3, 7 and 11 days reduced the number of CD163 positive hepatic resident macrophages (Kupffer cells) in normal livers, while increasing AST and ALT levels. In TAA (300 mg/kg BW)-treated rats injected with DX (0.5 mg/kg BW) pretreatment, the number of M1 and M2 macrophages showed a significant decrease compared with that of TAA-treated rats without DX treatment. Additionally, reparative fibrosis resulting from hepatocyte injury induced by TAA injection was suppressed by DX pretreatment. Our data suggested that macrophages could influence not only normal hepatic homeostasis (reflected by AST and ALT levels) but also chemically induced hepatic lesion development (reduced reparative fibrosis).
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spelling pubmed-76776302020-11-24 Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats Hada, Noa Kuramochi, Mizuki Izawa, Takeshi Kuwamura, Mitsuru Yamate, Jyoji J Toxicol Pathol Original Article Resident and infiltrative macrophages play important roles in the development of pathological lesions. M1/M2 macrophage polarization with respective CD68 and CD163 expression remains unclear in chemically induced liver injury. This study was aimed at investigating the influence of macrophages on normal and chemically induced liver injury. For this, dexamethasone (DX), an immunosuppressive drug, was administered in normal rats and thioacetamide (TAA)-treated rats. Liver samples were collected and analyzed with immunohistochemical methods. Repeated injections of DX (0.5 or 1.0 mg/kg BW) for 3, 7 and 11 days reduced the number of CD163 positive hepatic resident macrophages (Kupffer cells) in normal livers, while increasing AST and ALT levels. In TAA (300 mg/kg BW)-treated rats injected with DX (0.5 mg/kg BW) pretreatment, the number of M1 and M2 macrophages showed a significant decrease compared with that of TAA-treated rats without DX treatment. Additionally, reparative fibrosis resulting from hepatocyte injury induced by TAA injection was suppressed by DX pretreatment. Our data suggested that macrophages could influence not only normal hepatic homeostasis (reflected by AST and ALT levels) but also chemically induced hepatic lesion development (reduced reparative fibrosis). Japanese Society of Toxicologic Pathology 2020-07-31 2020-10 /pmc/articles/PMC7677630/ /pubmed/33239842 http://dx.doi.org/10.1293/tox.2020-0016 Text en ©2020 The Japanese Society of Toxicologic Pathology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Hada, Noa
Kuramochi, Mizuki
Izawa, Takeshi
Kuwamura, Mitsuru
Yamate, Jyoji
Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats
title Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats
title_full Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats
title_fullStr Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats
title_full_unstemmed Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats
title_short Effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats
title_sort effects of dexamethasone on hepatic macrophages in normal livers and thioacetamide-induced acute liver lesions in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677630/
https://www.ncbi.nlm.nih.gov/pubmed/33239842
http://dx.doi.org/10.1293/tox.2020-0016
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