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The attachment process and physiological properties of Escherichia coli O157:H7 on quartz

BACKGROUND: Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157:H7 has been studied, a compreh...

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Autores principales: Wang, Liliang, Wu, Yichao, Cai, Peng, Huang, Qiaoyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677791/
https://www.ncbi.nlm.nih.gov/pubmed/33213384
http://dx.doi.org/10.1186/s12866-020-02043-8
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author Wang, Liliang
Wu, Yichao
Cai, Peng
Huang, Qiaoyun
author_facet Wang, Liliang
Wu, Yichao
Cai, Peng
Huang, Qiaoyun
author_sort Wang, Liliang
collection PubMed
description BACKGROUND: Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157:H7 has been studied, a comprehensive understanding of the attachment process and physiological properties after attachment is still lacking. RESULTS: In this study, planktonic and attached Escherichia coli O157:H7 cells on quartz were investigated using RNA sequencing (RNA-seq) and the isobaric tagging for relative and absolute quantitation (iTRAQ) proteomic method. Based on the transcriptomic and proteomic analyses and gene knockouts, functional two-component system pathways were required for efficient attachment; chemotaxis and the Rcs system were identified to play determinant roles in E. coli O157:H7 attachment on quartz. After attachment, the pyruvate catabolic pathway shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route. The survival rate of attached E. coli O157:H7 increased more than 10-fold under penicillin and vancomycin stress and doubled under alkaline pH and ferric iron stress. CONCLUSIONS: These results contribute to the understanding of the roles of chemotaxis and the Rcs system in the attachment process of pathogens and indicate that the attachment of pathogens to minerals significantly elevates their resistance to antibiotics and environmental stress, which may pose a potential threat to public health. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-020-02043-8.
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spelling pubmed-76777912020-11-20 The attachment process and physiological properties of Escherichia coli O157:H7 on quartz Wang, Liliang Wu, Yichao Cai, Peng Huang, Qiaoyun BMC Microbiol Research Article BACKGROUND: Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157:H7 has been studied, a comprehensive understanding of the attachment process and physiological properties after attachment is still lacking. RESULTS: In this study, planktonic and attached Escherichia coli O157:H7 cells on quartz were investigated using RNA sequencing (RNA-seq) and the isobaric tagging for relative and absolute quantitation (iTRAQ) proteomic method. Based on the transcriptomic and proteomic analyses and gene knockouts, functional two-component system pathways were required for efficient attachment; chemotaxis and the Rcs system were identified to play determinant roles in E. coli O157:H7 attachment on quartz. After attachment, the pyruvate catabolic pathway shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route. The survival rate of attached E. coli O157:H7 increased more than 10-fold under penicillin and vancomycin stress and doubled under alkaline pH and ferric iron stress. CONCLUSIONS: These results contribute to the understanding of the roles of chemotaxis and the Rcs system in the attachment process of pathogens and indicate that the attachment of pathogens to minerals significantly elevates their resistance to antibiotics and environmental stress, which may pose a potential threat to public health. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-020-02043-8. BioMed Central 2020-11-19 /pmc/articles/PMC7677791/ /pubmed/33213384 http://dx.doi.org/10.1186/s12866-020-02043-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wang, Liliang
Wu, Yichao
Cai, Peng
Huang, Qiaoyun
The attachment process and physiological properties of Escherichia coli O157:H7 on quartz
title The attachment process and physiological properties of Escherichia coli O157:H7 on quartz
title_full The attachment process and physiological properties of Escherichia coli O157:H7 on quartz
title_fullStr The attachment process and physiological properties of Escherichia coli O157:H7 on quartz
title_full_unstemmed The attachment process and physiological properties of Escherichia coli O157:H7 on quartz
title_short The attachment process and physiological properties of Escherichia coli O157:H7 on quartz
title_sort attachment process and physiological properties of escherichia coli o157:h7 on quartz
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677791/
https://www.ncbi.nlm.nih.gov/pubmed/33213384
http://dx.doi.org/10.1186/s12866-020-02043-8
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