Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit

There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested negative by SARS-CoV-2 PCR, we investigated the se...

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Autores principales: Krüttgen, Alexander, Cornelissen, Christian G., Dreher, Michael, Hornef, Mathias W., Imöhl, Matthias, Kleines, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678421/
https://www.ncbi.nlm.nih.gov/pubmed/33227341
http://dx.doi.org/10.1016/j.jviromet.2020.114024
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author Krüttgen, Alexander
Cornelissen, Christian G.
Dreher, Michael
Hornef, Mathias W.
Imöhl, Matthias
Kleines, Michael
author_facet Krüttgen, Alexander
Cornelissen, Christian G.
Dreher, Michael
Hornef, Mathias W.
Imöhl, Matthias
Kleines, Michael
author_sort Krüttgen, Alexander
collection PubMed
description There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Rapid Antigen Test (Roche). We determined a specificity of 96 %. The assay’s sensitivity with samples with a cycle threshold of < 25, 25 - <30, 30 - <35, and> = 35 was 100 %, 95 %, 44.8 % and 22.2 %, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. However, the antigen assay may be a quick and easy to perform alternative for differentiation of individuals contagious for SARS-CoV-2 from non-contagious individuals.
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spelling pubmed-76784212020-11-23 Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit Krüttgen, Alexander Cornelissen, Christian G. Dreher, Michael Hornef, Mathias W. Imöhl, Matthias Kleines, Michael J Virol Methods Short Communication There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Rapid Antigen Test (Roche). We determined a specificity of 96 %. The assay’s sensitivity with samples with a cycle threshold of < 25, 25 - <30, 30 - <35, and> = 35 was 100 %, 95 %, 44.8 % and 22.2 %, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. However, the antigen assay may be a quick and easy to perform alternative for differentiation of individuals contagious for SARS-CoV-2 from non-contagious individuals. Elsevier B.V. 2021-02 2020-11-20 /pmc/articles/PMC7678421/ /pubmed/33227341 http://dx.doi.org/10.1016/j.jviromet.2020.114024 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Krüttgen, Alexander
Cornelissen, Christian G.
Dreher, Michael
Hornef, Mathias W.
Imöhl, Matthias
Kleines, Michael
Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit
title Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit
title_full Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit
title_fullStr Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit
title_full_unstemmed Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit
title_short Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit
title_sort comparison of the sars-cov-2 rapid antigen test to the real star sars-cov-2 rt pcr kit
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678421/
https://www.ncbi.nlm.nih.gov/pubmed/33227341
http://dx.doi.org/10.1016/j.jviromet.2020.114024
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