Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation

Spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-SP, SARS-CoV-2-NP) are the main immunogenic targets for antibodies. We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. In many cases, this can lead to false-...

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Autores principales: Rump, Airi, Risti, Robert, Kristal, Mai-Ly, Reut, Jekaterina, Syritski, Vitali, Lookene, Aivar, Ruutel Boudinot, Sirje
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678427/
https://www.ncbi.nlm.nih.gov/pubmed/33276951
http://dx.doi.org/10.1016/j.bbrc.2020.11.060
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author Rump, Airi
Risti, Robert
Kristal, Mai-Ly
Reut, Jekaterina
Syritski, Vitali
Lookene, Aivar
Ruutel Boudinot, Sirje
author_facet Rump, Airi
Risti, Robert
Kristal, Mai-Ly
Reut, Jekaterina
Syritski, Vitali
Lookene, Aivar
Ruutel Boudinot, Sirje
author_sort Rump, Airi
collection PubMed
description Spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-SP, SARS-CoV-2-NP) are the main immunogenic targets for antibodies. We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. In many cases, this can lead to false-negative serological tests. Deglycosylation of SARS-CoV-2-NP significantly increased the number of positive tests. The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes.
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spelling pubmed-76784272020-11-23 Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation Rump, Airi Risti, Robert Kristal, Mai-Ly Reut, Jekaterina Syritski, Vitali Lookene, Aivar Ruutel Boudinot, Sirje Biochem Biophys Res Commun Article Spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-SP, SARS-CoV-2-NP) are the main immunogenic targets for antibodies. We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. In many cases, this can lead to false-negative serological tests. Deglycosylation of SARS-CoV-2-NP significantly increased the number of positive tests. The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes. Elsevier Inc. 2021-01-01 2020-11-20 /pmc/articles/PMC7678427/ /pubmed/33276951 http://dx.doi.org/10.1016/j.bbrc.2020.11.060 Text en © 2020 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Rump, Airi
Risti, Robert
Kristal, Mai-Ly
Reut, Jekaterina
Syritski, Vitali
Lookene, Aivar
Ruutel Boudinot, Sirje
Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
title Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
title_full Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
title_fullStr Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
title_full_unstemmed Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
title_short Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation
title_sort dual elisa using sars-cov-2 nucleocapsid protein produced in e. coli and cho cells reveals epitope masking by n-glycosylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678427/
https://www.ncbi.nlm.nih.gov/pubmed/33276951
http://dx.doi.org/10.1016/j.bbrc.2020.11.060
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