Cargando…

Dual ELISA using SARS-CoV-2 nucleocapsid protein produced in E. coli and CHO cells reveals epitope masking by N-glycosylation

Spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-SP, SARS-CoV-2-NP) are the main immunogenic targets for antibodies. We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. In many cases, this can lead to false-...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rump, Airi, Risti, Robert, Kristal, Mai-Ly, Reut, Jekaterina, Syritski, Vitali, Lookene, Aivar, Ruutel Boudinot, Sirje
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Inc. 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678427/ https://www.ncbi.nlm.nih.gov/pubmed/33276951 http://dx.doi.org/10.1016/j.bbrc.2020.11.060

Ejemplares similares

Evolutionary Origin of the P2X7 C-ter Region: Capture of an Ancient Ballast Domain by a P2X4-Like Gene in Ancient Jawed Vertebrates
por: Rump, Airi, et al.
Publicado: (2020)

Lipoprotein Lipase Activity Does Not Differ in the Serum Environment of Vegans and Omnivores
por: Seeba, Natjan-Naatan, et al.
Publicado: (2023)

Porcine circovirus type 2 ORF3 protein induces apoptosis in melanoma cells
por: Teras, Marina, et al.
Publicado: (2018)

P2X4: A fast and sensitive purinergic receptor
por: Suurväli, Jaanus, et al.
Publicado: (2017)

Molecularly imprinted polymer based electrochemical sensor for quantitative detection of SARS-CoV-2 spike protein
por: Ayankojo, Akinrinade George, et al.
Publicado: (2022)

Development of a portable MIP-based electrochemical sensor for detection of SARS-CoV-2 antigen
por: Raziq, Abdul, et al.
Publicado: (2021)

Advances in Detection of Antibiotic Pollutants in Aqueous Media Using Molecular Imprinting Technique—A Review
por: Ayankojo, Akinrinade George, et al.
Publicado: (2022)

Human Peripheral Blood Eosinophils Express High Levels of the Purinergic Receptor P2X4
por: Paalme, Viiu, et al.
Publicado: (2019)

Combined action of albumin and heparin regulates lipoprotein lipase oligomerization, stability, and ligand interactions
por: Risti, Robert, et al.
Publicado: (2023)

Structural and Functional Features of the P2X4 Receptor: An Immunological Perspective
por: Kanellopoulos, Jean M., et al.
Publicado: (2021)

Controllability Analysis of Protein Glycosylation in Cho Cells
por: St. Amand, Melissa M., et al.
Publicado: (2014)

Identification of an epitope of SARS-coronavirus nucleocapsid protein
por: LIN, Ying, et al.
Publicado: (2003)

Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293
por: Porosk, Ly, et al.
Publicado: (2022)

Profiling of glycosylation gene expression in CHO fed-batch cultures in response to glycosylation-enhancing medium components
por: Boniface, Ryan, et al.
Publicado: (2013)

The Epitope Study on the SARS-CoV Nucleocapsid Protein
por: Li, Shuting, et al.
Publicado: (2003)

The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
por: Tian, Weihua, et al.
Publicado: (2019)

What can mathematical modelling say about CHO metabolism and protein glycosylation?
por: Galleguillos, Sarah N., et al.
Publicado: (2017)

Osmolality Effects on CHO Cell Growth, Cell Volume, Antibody Productivity and Glycosylation
por: Alhuthali, Sakhr, et al.
Publicado: (2021)

Engineering protein glycosylation in CHO cells to be highly similar to murine host cells
por: Gupta, Shivani, et al.
Publicado: (2023)

Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay*
por: DALBY, TINE, et al.
Publicado: (2010)

Development of a Nucleocapsid Protein-Based Blocking ELISA for the Detection of Porcine Deltacoronavirus Antibodies
por: Wang, Wenlong, et al.
Publicado: (2022)

Characterization of monoclonal antibody against SARS coronavirus nucleocapsid antigen and development of an antigen capture ELISA
por: He, Qigai, et al.
Publicado: (2005)

The Lec4A CHO glycosylation mutant arises from miscompartmentalization of a Golgi glycosyltransferase
Publicado: (1989)

Glycosylation of bacterial antigens changes epitope patterns
por: Kern, Karolin, et al.
Publicado: (2023)

Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies
por: Liu, Bo, et al.
Publicado: (2013)

Glycoproteomic Characterization of FUT8 Knock-Out CHO Cells Reveals Roles of FUT8 in the Glycosylation
por: Yang, Ganglong, et al.
Publicado: (2021)

Identification of a novel B cell epitope on the nucleocapsid protein of porcine deltacoronavirus
por: Wei, Shan, et al.
Publicado: (2021)

Identification of two novel B-cell epitopes on the nucleocapsid protein of porcine deltacoronavirus
por: Ren, Haojie, et al.
Publicado: (2022)

Identification of Conserved Epitopes in SARS-CoV-2 Spike and Nucleocapsid Protein
por: Forcelloni, Sergio, et al.
Publicado: (2021)

ELISA for Determination of Human Growth Hormone: Recognition of Helix 4 Epitopes
por: Moura, Juliana F., et al.
Publicado: (2004)

Epitope Mapping of the Diphtheria Toxin and Development of an ELISA-Specific Diagnostic Assay
por: De-Simone, Salvatore Giovanni, et al.
Publicado: (2021)

Dimerization of SARS-CoV-2 nucleocapsid protein affects sensitivity of ELISA based diagnostics of COVID-19
por: Khan, Wajihul Hasan, et al.
Publicado: (2022)

Development of nucleocapsid-specific monoclonal antibodies for SARS-CoV-2 and their ELISA diagnostics on an automatic microfluidic device
por: Yang, Jihyun, et al.
Publicado: (2023)

Comparison of nucleocapsid and spike antibody ELISAs for determining SARS‐CoV‐2 seropositivity in Kenyan women and infants
por: Fish, Carolyn S., et al.
Publicado: (2022)

Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells
por: Burleigh, Susan C, et al.
Publicado: (2011)

Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens
por: Li, Sophia W., et al.
Publicado: (2020)

The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea Virus
por: Wang, Kang, et al.
Publicado: (2016)

Identification of a Novel Linear B-Cell Epitope on the Nucleocapsid Protein of Porcine Deltacoronavirus
por: Fu, Jiayu, et al.
Publicado: (2020)

In silico multi-epitope Bunyumwera virus vaccine to target virus nucleocapsid N protein
por: Nelluri, Kanaka Durga Devi, et al.
Publicado: (2022)

Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein
por: He, Wei, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_hd1v8dkh9rnb89nbkcie5e5lg9