Cargando…

Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster

Potassium (K(+)) acquisition, translocation and cellular homeostasis are mediated by various membrane transport systems in all organisms. We identified and described an ion channel in the ectomycorrhizal fungus Hebeloma cylindrosporum (HcSKC) that harbors features of animal voltage-dependent Shaker-...

Descripción completa

Detalles Bibliográficos
Autores principales: Garcia, Kevin, Guerrero-Galán, Carmen, Frank, Hannah E. R., Haider, Muhammad Zulqurnain, Delteil, Amandine, Conéjéro, Geneviève, Lambilliotte, Raphaël, Fizames, Cécile, Sentenac, Hervé, Zimmermann, Sabine D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678990/
https://www.ncbi.nlm.nih.gov/pubmed/33216794
http://dx.doi.org/10.1371/journal.pone.0242739
_version_ 1783612261199249408
author Garcia, Kevin
Guerrero-Galán, Carmen
Frank, Hannah E. R.
Haider, Muhammad Zulqurnain
Delteil, Amandine
Conéjéro, Geneviève
Lambilliotte, Raphaël
Fizames, Cécile
Sentenac, Hervé
Zimmermann, Sabine D.
author_facet Garcia, Kevin
Guerrero-Galán, Carmen
Frank, Hannah E. R.
Haider, Muhammad Zulqurnain
Delteil, Amandine
Conéjéro, Geneviève
Lambilliotte, Raphaël
Fizames, Cécile
Sentenac, Hervé
Zimmermann, Sabine D.
author_sort Garcia, Kevin
collection PubMed
description Potassium (K(+)) acquisition, translocation and cellular homeostasis are mediated by various membrane transport systems in all organisms. We identified and described an ion channel in the ectomycorrhizal fungus Hebeloma cylindrosporum (HcSKC) that harbors features of animal voltage-dependent Shaker-like K(+) channels, and investigated its role in both free-living hyphae and symbiotic conditions. RNAi lines affected in the expression of HcSKC were produced and used for in vitro mycorrhizal assays with the maritime pine as host plant, under standard or low K(+) conditions. The adaptation of H. cylindrosporum to the downregulation of HcSKC was analyzed by qRT-PCR analyses for other K(+)-related transport proteins: the transporters HcTrk1, HcTrk2, and HcHAK, and the ion channels HcTOK1, HcTOK2.1, and HcTOK2.2. Downregulated HcSKC transformants displayed greater K(+) contents at standard K(+) only. In such conditions, plants inoculated with these transgenic lines were impaired in K(+) nutrition. Taken together, these results support the hypothesis that the reduced expression of HcSKC modifies the pool of fungal K(+) available for the plant and/or affects its symbiotic transfer to the roots. Our study reveals that the maintenance of K(+) transport in H. cylindrosporum, through the regulation of HcSKC expression, is required for the K(+) nutrition of the host plant.
format Online
Article
Text
id pubmed-7678990
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-76789902020-12-02 Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster Garcia, Kevin Guerrero-Galán, Carmen Frank, Hannah E. R. Haider, Muhammad Zulqurnain Delteil, Amandine Conéjéro, Geneviève Lambilliotte, Raphaël Fizames, Cécile Sentenac, Hervé Zimmermann, Sabine D. PLoS One Research Article Potassium (K(+)) acquisition, translocation and cellular homeostasis are mediated by various membrane transport systems in all organisms. We identified and described an ion channel in the ectomycorrhizal fungus Hebeloma cylindrosporum (HcSKC) that harbors features of animal voltage-dependent Shaker-like K(+) channels, and investigated its role in both free-living hyphae and symbiotic conditions. RNAi lines affected in the expression of HcSKC were produced and used for in vitro mycorrhizal assays with the maritime pine as host plant, under standard or low K(+) conditions. The adaptation of H. cylindrosporum to the downregulation of HcSKC was analyzed by qRT-PCR analyses for other K(+)-related transport proteins: the transporters HcTrk1, HcTrk2, and HcHAK, and the ion channels HcTOK1, HcTOK2.1, and HcTOK2.2. Downregulated HcSKC transformants displayed greater K(+) contents at standard K(+) only. In such conditions, plants inoculated with these transgenic lines were impaired in K(+) nutrition. Taken together, these results support the hypothesis that the reduced expression of HcSKC modifies the pool of fungal K(+) available for the plant and/or affects its symbiotic transfer to the roots. Our study reveals that the maintenance of K(+) transport in H. cylindrosporum, through the regulation of HcSKC expression, is required for the K(+) nutrition of the host plant. Public Library of Science 2020-11-20 /pmc/articles/PMC7678990/ /pubmed/33216794 http://dx.doi.org/10.1371/journal.pone.0242739 Text en © 2020 Garcia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Garcia, Kevin
Guerrero-Galán, Carmen
Frank, Hannah E. R.
Haider, Muhammad Zulqurnain
Delteil, Amandine
Conéjéro, Geneviève
Lambilliotte, Raphaël
Fizames, Cécile
Sentenac, Hervé
Zimmermann, Sabine D.
Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster
title Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster
title_full Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster
title_fullStr Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster
title_full_unstemmed Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster
title_short Fungal Shaker-like channels beyond cellular K(+) homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster
title_sort fungal shaker-like channels beyond cellular k(+) homeostasis: a role in ectomycorrhizal symbiosis between hebeloma cylindrosporum and pinus pinaster
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678990/
https://www.ncbi.nlm.nih.gov/pubmed/33216794
http://dx.doi.org/10.1371/journal.pone.0242739
work_keys_str_mv AT garciakevin fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT guerrerogalancarmen fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT frankhannaher fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT haidermuhammadzulqurnain fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT delteilamandine fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT conejerogenevieve fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT lambilliotteraphael fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT fizamescecile fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT sentenacherve fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster
AT zimmermannsabined fungalshakerlikechannelsbeyondcellularkhomeostasisaroleinectomycorrhizalsymbiosisbetweenhebelomacylindrosporumandpinuspinaster