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Effects of Rhein on Bile Acid Homeostasis in Rats

Rhein, the active ingredient of rhubarb, a medicinal and edible plant, is widely used in clinical practice. However, the effects of repeated intake of rhein on liver function and bile acid metabolism are rarely reported. In this work, we investigated the alterations of 14 bile acids and hepatic tran...

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Autores principales: Xian, Zhong, Tian, Jingzhuo, Wang, Lianmei, Zhang, Yushi, Han, Jiayin, Deng, Nuo, Liu, Suyan, Zhao, Yong, Li, Chunyin, Yi, Yan, Wang, Dunfang, Meng, Jing, Pan, Chen, Liang, Aihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679202/
https://www.ncbi.nlm.nih.gov/pubmed/33274227
http://dx.doi.org/10.1155/2020/8827955
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author Xian, Zhong
Tian, Jingzhuo
Wang, Lianmei
Zhang, Yushi
Han, Jiayin
Deng, Nuo
Liu, Suyan
Zhao, Yong
Li, Chunyin
Yi, Yan
Wang, Dunfang
Meng, Jing
Pan, Chen
Liang, Aihua
author_facet Xian, Zhong
Tian, Jingzhuo
Wang, Lianmei
Zhang, Yushi
Han, Jiayin
Deng, Nuo
Liu, Suyan
Zhao, Yong
Li, Chunyin
Yi, Yan
Wang, Dunfang
Meng, Jing
Pan, Chen
Liang, Aihua
author_sort Xian, Zhong
collection PubMed
description Rhein, the active ingredient of rhubarb, a medicinal and edible plant, is widely used in clinical practice. However, the effects of repeated intake of rhein on liver function and bile acid metabolism are rarely reported. In this work, we investigated the alterations of 14 bile acids and hepatic transporters after rats were administered with rhein for 5 weeks. There was no obvious injury to the liver and kidney, and there were no significant changes in biochemical indicators. However, 1,000 mg/kg rhein increased the liver total bile acid (TBA) levels, especially taurine-conjugated bile acids (t-CBAs), inhibited the expression of farnesoid X receptor (FXR), small heterodimer partner (SHP), and bile salt export pump (BSEP) mRNA, and upregulated the expression of (cholesterol 7α-hydroxylase) CYP7A1 mRNA. Rhein close to the clinical dose (10 mg/kg and 30 mg/kg) reduced the amounts of TBAs, especially unconjugated bile acids (UCBAs), and elevated the expression of FXR and multidrug resistance-associated protein 3 (Mrp3) mRNA. These results denote that rhein is relatively safe to use at a reasonable dose and timing. 30 mg/kg rhein may promote bile acid transport and reduce bile acid accumulation by upregulating the expression of FXR mRNA and Mrp3 mRNA, potentially resulting in the decrease in serum UBCAs.
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spelling pubmed-76792022020-12-02 Effects of Rhein on Bile Acid Homeostasis in Rats Xian, Zhong Tian, Jingzhuo Wang, Lianmei Zhang, Yushi Han, Jiayin Deng, Nuo Liu, Suyan Zhao, Yong Li, Chunyin Yi, Yan Wang, Dunfang Meng, Jing Pan, Chen Liang, Aihua Biomed Res Int Research Article Rhein, the active ingredient of rhubarb, a medicinal and edible plant, is widely used in clinical practice. However, the effects of repeated intake of rhein on liver function and bile acid metabolism are rarely reported. In this work, we investigated the alterations of 14 bile acids and hepatic transporters after rats were administered with rhein for 5 weeks. There was no obvious injury to the liver and kidney, and there were no significant changes in biochemical indicators. However, 1,000 mg/kg rhein increased the liver total bile acid (TBA) levels, especially taurine-conjugated bile acids (t-CBAs), inhibited the expression of farnesoid X receptor (FXR), small heterodimer partner (SHP), and bile salt export pump (BSEP) mRNA, and upregulated the expression of (cholesterol 7α-hydroxylase) CYP7A1 mRNA. Rhein close to the clinical dose (10 mg/kg and 30 mg/kg) reduced the amounts of TBAs, especially unconjugated bile acids (UCBAs), and elevated the expression of FXR and multidrug resistance-associated protein 3 (Mrp3) mRNA. These results denote that rhein is relatively safe to use at a reasonable dose and timing. 30 mg/kg rhein may promote bile acid transport and reduce bile acid accumulation by upregulating the expression of FXR mRNA and Mrp3 mRNA, potentially resulting in the decrease in serum UBCAs. Hindawi 2020-11-09 /pmc/articles/PMC7679202/ /pubmed/33274227 http://dx.doi.org/10.1155/2020/8827955 Text en Copyright © 2020 Zhong Xian et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xian, Zhong
Tian, Jingzhuo
Wang, Lianmei
Zhang, Yushi
Han, Jiayin
Deng, Nuo
Liu, Suyan
Zhao, Yong
Li, Chunyin
Yi, Yan
Wang, Dunfang
Meng, Jing
Pan, Chen
Liang, Aihua
Effects of Rhein on Bile Acid Homeostasis in Rats
title Effects of Rhein on Bile Acid Homeostasis in Rats
title_full Effects of Rhein on Bile Acid Homeostasis in Rats
title_fullStr Effects of Rhein on Bile Acid Homeostasis in Rats
title_full_unstemmed Effects of Rhein on Bile Acid Homeostasis in Rats
title_short Effects of Rhein on Bile Acid Homeostasis in Rats
title_sort effects of rhein on bile acid homeostasis in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679202/
https://www.ncbi.nlm.nih.gov/pubmed/33274227
http://dx.doi.org/10.1155/2020/8827955
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