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The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz)
Rutin, a well-known bioflavonoid, was found abundantly in cassava leaves. In the present study, extraction techniques including maceration, boiling, reflux, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) were optimised to increase the yield of rutin. Extraction paramet...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679431/ https://www.ncbi.nlm.nih.gov/pubmed/33250654 http://dx.doi.org/10.1016/j.jsps.2020.09.012 |
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author | Chahyadi, Agus Elfahmi |
author_facet | Chahyadi, Agus Elfahmi |
author_sort | Chahyadi, Agus |
collection | PubMed |
description | Rutin, a well-known bioflavonoid, was found abundantly in cassava leaves. In the present study, extraction techniques including maceration, boiling, reflux, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) were optimised to increase the yield of rutin. Extraction parameters such as solvents, solid-liquid ratio, temperature, and time were optimised to give better extraction yields for each method. HPLC analysis showed a high content of rutin which is up to 2.4% per dry weight of cassava leaves. The extraction yields under optimised condition were found to be 16.00 ± 0.21, 20.38 ± 0.66, 22.33 ± 2.3, 24.49 ± 0.41, and 23.37 ± 1.00 g rutin per kg dry weight for maceration, boiling, reflux, UAE and MAE methods, respectively. Specifically, UAE reduced the extraction time to 90 min, using only 40–60% of aqueous ethanol. Meanwhile, MAE completed the extraction under 5 min and no significant differences in output was observed between the use of water and aqueous ethanol. Accordingly, with the extraction efficiency of up to 99 and 94%, respectively, both processes provided better results. The subsequent green purification using chilling method produced a typical cassava bioflavonoid containing 82% of rutin and 17% of nicotiflorin. This study informs a new abundant source of rutin and provides the optimum condition of extraction methods for high yield of rutin from cassava leaves. |
format | Online Article Text |
id | pubmed-7679431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-76794312020-11-27 The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz) Chahyadi, Agus Elfahmi Saudi Pharm J Original Article Rutin, a well-known bioflavonoid, was found abundantly in cassava leaves. In the present study, extraction techniques including maceration, boiling, reflux, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) were optimised to increase the yield of rutin. Extraction parameters such as solvents, solid-liquid ratio, temperature, and time were optimised to give better extraction yields for each method. HPLC analysis showed a high content of rutin which is up to 2.4% per dry weight of cassava leaves. The extraction yields under optimised condition were found to be 16.00 ± 0.21, 20.38 ± 0.66, 22.33 ± 2.3, 24.49 ± 0.41, and 23.37 ± 1.00 g rutin per kg dry weight for maceration, boiling, reflux, UAE and MAE methods, respectively. Specifically, UAE reduced the extraction time to 90 min, using only 40–60% of aqueous ethanol. Meanwhile, MAE completed the extraction under 5 min and no significant differences in output was observed between the use of water and aqueous ethanol. Accordingly, with the extraction efficiency of up to 99 and 94%, respectively, both processes provided better results. The subsequent green purification using chilling method produced a typical cassava bioflavonoid containing 82% of rutin and 17% of nicotiflorin. This study informs a new abundant source of rutin and provides the optimum condition of extraction methods for high yield of rutin from cassava leaves. Elsevier 2020-11 2020-09-22 /pmc/articles/PMC7679431/ /pubmed/33250654 http://dx.doi.org/10.1016/j.jsps.2020.09.012 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Chahyadi, Agus Elfahmi The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz) |
title | The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz) |
title_full | The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz) |
title_fullStr | The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz) |
title_full_unstemmed | The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz) |
title_short | The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz) |
title_sort | influence of extraction methods on rutin yield of cassava leaves (manihot esculenta crantz) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679431/ https://www.ncbi.nlm.nih.gov/pubmed/33250654 http://dx.doi.org/10.1016/j.jsps.2020.09.012 |
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