Cargando…

Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group

We examined four suspected cases of facultative parthenogenesis in three species of a neotropical lineage of pitvipers of the Bothrops atrox group. Reproduction without mating was observed in captive females of B. atrox, B. moojeni and B. leucurus housed alone for seven years (the two former species...

Descripción completa

Detalles Bibliográficos
Autores principales: Cubides-Cubillos, Sergio D., Patané, José S.L., Pereira da Silva, Karina Maria, Almeida-Santos, Selma Maria, Polydoro, Denise S., Galassi, Guilherme Guidolin, Travaglia Cardoso, Silvia Regina, Silva, Maria José de J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680053/
https://www.ncbi.nlm.nih.gov/pubmed/33240594
http://dx.doi.org/10.7717/peerj.10097
_version_ 1783612385628520448
author Cubides-Cubillos, Sergio D.
Patané, José S.L.
Pereira da Silva, Karina Maria
Almeida-Santos, Selma Maria
Polydoro, Denise S.
Galassi, Guilherme Guidolin
Travaglia Cardoso, Silvia Regina
Silva, Maria José de J.
author_facet Cubides-Cubillos, Sergio D.
Patané, José S.L.
Pereira da Silva, Karina Maria
Almeida-Santos, Selma Maria
Polydoro, Denise S.
Galassi, Guilherme Guidolin
Travaglia Cardoso, Silvia Regina
Silva, Maria José de J.
author_sort Cubides-Cubillos, Sergio D.
collection PubMed
description We examined four suspected cases of facultative parthenogenesis in three species of a neotropical lineage of pitvipers of the Bothrops atrox group. Reproduction without mating was observed in captive females of B. atrox, B. moojeni and B. leucurus housed alone for seven years (the two former species) and nine years (the latter one). In addition to the observation of captivity data, we investigated molecularly this phenomenon using heterologous microsatellites. DNA was extracted from the mothers’ scales or liver, from embryo and newborn fragments, and yolked ova. Four of the microsatellites showed good amplification using Polymerase Chain Reaction and informative band segregation patterns among each mother and respective offspring. Captivity information, litter characteristics (comparison of the number of newborns, embryos and yolked ova) and molecular data altogether agreed with facultative parthenogenesis predictions in at least three out of the four mothers studied: B. atrox (ID#933) was heterozygous for three out of the four markers, and the sons S1 and S2 were homozygous; B. moojeni (BUT86) was heterozygous for two out of four markers, offspring S1, S3, E2, and E4, and O1 to O6 were homozygous; and B. leucurus (MJJS503) was heterozygous for three out of four markers, and son E1 and O1 were homozygous. B. moojeni (BUT44) was homozygous for all loci analyzed in the mother and offspring, which although not informative is also consistent with parthenogenesis. This study represents the first molecular confirmation of different pitviper species undergoing facultative parthenogenesis among Neotropical endemic snakes.
format Online
Article
Text
id pubmed-7680053
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-76800532020-11-24 Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group Cubides-Cubillos, Sergio D. Patané, José S.L. Pereira da Silva, Karina Maria Almeida-Santos, Selma Maria Polydoro, Denise S. Galassi, Guilherme Guidolin Travaglia Cardoso, Silvia Regina Silva, Maria José de J. PeerJ Biodiversity We examined four suspected cases of facultative parthenogenesis in three species of a neotropical lineage of pitvipers of the Bothrops atrox group. Reproduction without mating was observed in captive females of B. atrox, B. moojeni and B. leucurus housed alone for seven years (the two former species) and nine years (the latter one). In addition to the observation of captivity data, we investigated molecularly this phenomenon using heterologous microsatellites. DNA was extracted from the mothers’ scales or liver, from embryo and newborn fragments, and yolked ova. Four of the microsatellites showed good amplification using Polymerase Chain Reaction and informative band segregation patterns among each mother and respective offspring. Captivity information, litter characteristics (comparison of the number of newborns, embryos and yolked ova) and molecular data altogether agreed with facultative parthenogenesis predictions in at least three out of the four mothers studied: B. atrox (ID#933) was heterozygous for three out of the four markers, and the sons S1 and S2 were homozygous; B. moojeni (BUT86) was heterozygous for two out of four markers, offspring S1, S3, E2, and E4, and O1 to O6 were homozygous; and B. leucurus (MJJS503) was heterozygous for three out of four markers, and son E1 and O1 were homozygous. B. moojeni (BUT44) was homozygous for all loci analyzed in the mother and offspring, which although not informative is also consistent with parthenogenesis. This study represents the first molecular confirmation of different pitviper species undergoing facultative parthenogenesis among Neotropical endemic snakes. PeerJ Inc. 2020-11-18 /pmc/articles/PMC7680053/ /pubmed/33240594 http://dx.doi.org/10.7717/peerj.10097 Text en ©2020 Cubides-Cubillos et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biodiversity
Cubides-Cubillos, Sergio D.
Patané, José S.L.
Pereira da Silva, Karina Maria
Almeida-Santos, Selma Maria
Polydoro, Denise S.
Galassi, Guilherme Guidolin
Travaglia Cardoso, Silvia Regina
Silva, Maria José de J.
Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group
title Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group
title_full Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group
title_fullStr Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group
title_full_unstemmed Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group
title_short Evidence of facultative parthenogenesis in three Neotropical pitviper species of the Bothrops atrox group
title_sort evidence of facultative parthenogenesis in three neotropical pitviper species of the bothrops atrox group
topic Biodiversity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680053/
https://www.ncbi.nlm.nih.gov/pubmed/33240594
http://dx.doi.org/10.7717/peerj.10097
work_keys_str_mv AT cubidescubillossergiod evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup
AT patanejosesl evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup
AT pereiradasilvakarinamaria evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup
AT almeidasantosselmamaria evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup
AT polydorodenises evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup
AT galassiguilhermeguidolin evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup
AT travagliacardososilviaregina evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup
AT silvamariajosedej evidenceoffacultativeparthenogenesisinthreeneotropicalpitviperspeciesofthebothropsatroxgroup