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Novel viruses detected in bats in the Republic of Korea
Bats are natural reservoirs for potential zoonotic viruses. In this study, next-generation sequencing was performed to obtain entire genome sequences of picornavirus from a picornavirus-positive bat feces sample (16BF77) and to explore novel viruses in a pooled bat sample (16BP) from samples collect...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680115/ https://www.ncbi.nlm.nih.gov/pubmed/33219309 http://dx.doi.org/10.1038/s41598-020-77307-4 |
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author | Lee, Sook-Young Chung, Chul-Un Park, Jun Soo Oem, Jae-Ku |
author_facet | Lee, Sook-Young Chung, Chul-Un Park, Jun Soo Oem, Jae-Ku |
author_sort | Lee, Sook-Young |
collection | PubMed |
description | Bats are natural reservoirs for potential zoonotic viruses. In this study, next-generation sequencing was performed to obtain entire genome sequences of picornavirus from a picornavirus-positive bat feces sample (16BF77) and to explore novel viruses in a pooled bat sample (16BP) from samples collected in South Korea, 2016. Fourteen mammalian viral sequences were identified from 16BF77 and 29 from 16BP, and verified by RT-PCR. The most abundant virus in 16BF77 was picornavirus. Highly variable picornavirus sequences encoding 3D(pol) were classified into genera Kobuvirus, Shanbavirus, and an unassigned group within the family Picornaviridae. Amino acid differences between these partial 3D(pol) sequences were ≥ 65.7%. Results showed that one bat was co-infected by picornaviruses of more than two genera. Retrovirus, coronavirus, and rotavirus A sequences also were found in the BP sample. The retrovirus and coronavirus genomes were identified in nine and eight bats, respectively. Korean bat retroviruses and coronavirus demonstrated strong genetic relationships with a Chinese bat retrovirus (RfRV) and coronavirus (HKU5-1), respectively. A co-infection was identified in one bat with a retrovirus and a coronavirus. Our results indicate that Korean bats were multiply infected by several mammal viruses. |
format | Online Article Text |
id | pubmed-7680115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76801152020-11-24 Novel viruses detected in bats in the Republic of Korea Lee, Sook-Young Chung, Chul-Un Park, Jun Soo Oem, Jae-Ku Sci Rep Article Bats are natural reservoirs for potential zoonotic viruses. In this study, next-generation sequencing was performed to obtain entire genome sequences of picornavirus from a picornavirus-positive bat feces sample (16BF77) and to explore novel viruses in a pooled bat sample (16BP) from samples collected in South Korea, 2016. Fourteen mammalian viral sequences were identified from 16BF77 and 29 from 16BP, and verified by RT-PCR. The most abundant virus in 16BF77 was picornavirus. Highly variable picornavirus sequences encoding 3D(pol) were classified into genera Kobuvirus, Shanbavirus, and an unassigned group within the family Picornaviridae. Amino acid differences between these partial 3D(pol) sequences were ≥ 65.7%. Results showed that one bat was co-infected by picornaviruses of more than two genera. Retrovirus, coronavirus, and rotavirus A sequences also were found in the BP sample. The retrovirus and coronavirus genomes were identified in nine and eight bats, respectively. Korean bat retroviruses and coronavirus demonstrated strong genetic relationships with a Chinese bat retrovirus (RfRV) and coronavirus (HKU5-1), respectively. A co-infection was identified in one bat with a retrovirus and a coronavirus. Our results indicate that Korean bats were multiply infected by several mammal viruses. Nature Publishing Group UK 2020-11-20 /pmc/articles/PMC7680115/ /pubmed/33219309 http://dx.doi.org/10.1038/s41598-020-77307-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lee, Sook-Young Chung, Chul-Un Park, Jun Soo Oem, Jae-Ku Novel viruses detected in bats in the Republic of Korea |
title | Novel viruses detected in bats in the Republic of Korea |
title_full | Novel viruses detected in bats in the Republic of Korea |
title_fullStr | Novel viruses detected in bats in the Republic of Korea |
title_full_unstemmed | Novel viruses detected in bats in the Republic of Korea |
title_short | Novel viruses detected in bats in the Republic of Korea |
title_sort | novel viruses detected in bats in the republic of korea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680115/ https://www.ncbi.nlm.nih.gov/pubmed/33219309 http://dx.doi.org/10.1038/s41598-020-77307-4 |
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