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Proteomic assessment of serum biomarkers of longevity in older men
The biological bases of longevity are not well understood, and there are limited biomarkers for the prediction of long life. We used a high‐throughput, discovery‐based proteomics approach to identify serum peptides and proteins that were associated with the attainment of longevity in a longitudinal...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7681066/ https://www.ncbi.nlm.nih.gov/pubmed/33078901 http://dx.doi.org/10.1111/acel.13253 |
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author | Orwoll, Eric S. Wiedrick, Jack Nielson, Carrie M. Jacobs, Jon Baker, Erin S. Piehowski, Paul Petyuk, Vladislav Gao, Yuqian Shi, Tujin Smith, Richard D. Bauer, Douglas C. Cummings, Steven R. Lapidus, Jodi |
author_facet | Orwoll, Eric S. Wiedrick, Jack Nielson, Carrie M. Jacobs, Jon Baker, Erin S. Piehowski, Paul Petyuk, Vladislav Gao, Yuqian Shi, Tujin Smith, Richard D. Bauer, Douglas C. Cummings, Steven R. Lapidus, Jodi |
author_sort | Orwoll, Eric S. |
collection | PubMed |
description | The biological bases of longevity are not well understood, and there are limited biomarkers for the prediction of long life. We used a high‐throughput, discovery‐based proteomics approach to identify serum peptides and proteins that were associated with the attainment of longevity in a longitudinal study of community‐dwelling men age ≥65 years. Baseline serum in 1196 men were analyzed using liquid chromatography–ion mobility–mass spectrometry, and lifespan was determined during ~12 years of follow‐up. Men who achieved longevity (≥90% expected survival) were compared to those who died earlier. Rigorous statistical methods that controlled for false positivity were utilized to identify 25 proteins that were associated with longevity. All these proteins were in lower abundance in long‐lived men and included a variety involved in inflammation or complement activation. Lower levels of longevity‐associated proteins were also associated with better health status, but as time to death shortened, levels of these proteins increased. Pathway analyses implicated a number of compounds as important upstream regulators of the proteins and implicated shared networks that underlie the observed associations with longevity. Overall, these results suggest that complex pathways, prominently including inflammation, are linked to the likelihood of attaining longevity. This work may serve to identify novel biomarkers for longevity and to understand the biology underlying lifespan. |
format | Online Article Text |
id | pubmed-7681066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76810662020-11-27 Proteomic assessment of serum biomarkers of longevity in older men Orwoll, Eric S. Wiedrick, Jack Nielson, Carrie M. Jacobs, Jon Baker, Erin S. Piehowski, Paul Petyuk, Vladislav Gao, Yuqian Shi, Tujin Smith, Richard D. Bauer, Douglas C. Cummings, Steven R. Lapidus, Jodi Aging Cell Original Articles The biological bases of longevity are not well understood, and there are limited biomarkers for the prediction of long life. We used a high‐throughput, discovery‐based proteomics approach to identify serum peptides and proteins that were associated with the attainment of longevity in a longitudinal study of community‐dwelling men age ≥65 years. Baseline serum in 1196 men were analyzed using liquid chromatography–ion mobility–mass spectrometry, and lifespan was determined during ~12 years of follow‐up. Men who achieved longevity (≥90% expected survival) were compared to those who died earlier. Rigorous statistical methods that controlled for false positivity were utilized to identify 25 proteins that were associated with longevity. All these proteins were in lower abundance in long‐lived men and included a variety involved in inflammation or complement activation. Lower levels of longevity‐associated proteins were also associated with better health status, but as time to death shortened, levels of these proteins increased. Pathway analyses implicated a number of compounds as important upstream regulators of the proteins and implicated shared networks that underlie the observed associations with longevity. Overall, these results suggest that complex pathways, prominently including inflammation, are linked to the likelihood of attaining longevity. This work may serve to identify novel biomarkers for longevity and to understand the biology underlying lifespan. John Wiley and Sons Inc. 2020-10-20 2020-11 /pmc/articles/PMC7681066/ /pubmed/33078901 http://dx.doi.org/10.1111/acel.13253 Text en © 2020 The Authors. Aging Cell published by Anatomical Society and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Orwoll, Eric S. Wiedrick, Jack Nielson, Carrie M. Jacobs, Jon Baker, Erin S. Piehowski, Paul Petyuk, Vladislav Gao, Yuqian Shi, Tujin Smith, Richard D. Bauer, Douglas C. Cummings, Steven R. Lapidus, Jodi Proteomic assessment of serum biomarkers of longevity in older men |
title | Proteomic assessment of serum biomarkers of longevity in older men |
title_full | Proteomic assessment of serum biomarkers of longevity in older men |
title_fullStr | Proteomic assessment of serum biomarkers of longevity in older men |
title_full_unstemmed | Proteomic assessment of serum biomarkers of longevity in older men |
title_short | Proteomic assessment of serum biomarkers of longevity in older men |
title_sort | proteomic assessment of serum biomarkers of longevity in older men |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7681066/ https://www.ncbi.nlm.nih.gov/pubmed/33078901 http://dx.doi.org/10.1111/acel.13253 |
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