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Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016

Multidrug-resistant (MDR; resistance to ≥3 antimicrobial classes) members of the Pasteurellaceae family may compromise the efficacy of therapies used to prevent and treat bovine respiratory disease (BRD) in feedlot cattle. This study examined the prevalence of multidrug resistance in strains of Mann...

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Autores principales: Klima, Cassidy L., Holman, Devin B., Cook, Shaun R., Conrad, Cheyenne C., Ralston, Brenda J., Allan, Nick, Anholt, R. Michele, Niu, Yan D., Stanford, Kim, Hannon, Sherry J., Booker, Calvin W., McAllister, Tim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682020/
https://www.ncbi.nlm.nih.gov/pubmed/33240256
http://dx.doi.org/10.3389/fmicb.2020.606438
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author Klima, Cassidy L.
Holman, Devin B.
Cook, Shaun R.
Conrad, Cheyenne C.
Ralston, Brenda J.
Allan, Nick
Anholt, R. Michele
Niu, Yan D.
Stanford, Kim
Hannon, Sherry J.
Booker, Calvin W.
McAllister, Tim A.
author_facet Klima, Cassidy L.
Holman, Devin B.
Cook, Shaun R.
Conrad, Cheyenne C.
Ralston, Brenda J.
Allan, Nick
Anholt, R. Michele
Niu, Yan D.
Stanford, Kim
Hannon, Sherry J.
Booker, Calvin W.
McAllister, Tim A.
author_sort Klima, Cassidy L.
collection PubMed
description Multidrug-resistant (MDR; resistance to ≥3 antimicrobial classes) members of the Pasteurellaceae family may compromise the efficacy of therapies used to prevent and treat bovine respiratory disease (BRD) in feedlot cattle. This study examined the prevalence of multidrug resistance in strains of Mannheimia haemolytica and Pasteurella multocida collected from BRD cattle mortalities in North America. Isolates of M. haemolytica (n = 147) and P. multocida (n = 70) spanning 69 Alberta feedlots from 2011 to 2016 and two United States feedlots from 2011 to 2012 were examined for antimicrobial resistance (AMR) in association with integrative and conjugative elements (ICEs). Overall, resistance was high in both bacterial species with an increase in the prevalence of MDR isolates between 2011 and 2016. Resistance to >7 antimicrobial drugs occurred in 31% of M. haemolytica and 83% of P. multocida isolates. Resistance to sulfadimethoxine, trimethoprim/sulfamethoxazole, neomycin, clindamycin oxytetracycline, spectinomycin, tylosin, tilmicosin, and tulathromycin was most common. Although >80% of strains harbored three or more ICE-associated genes, only 12% of M. haemolytica and 77% of P. multocida contained all six, reflecting the diversity of ICEs. There was evidence of clonal spread as P. multocida and M. haemolytica isolates with the same pulsed-field gel electrophoresis profile from the United States in 2011 were isolated in Alberta in 2015–2016. This work highlights that MDR strains of Pasteurellaceae containing ICEs are widespread and may be contributing to BRD therapy failure in feedlot cattle. Given the antimicrobial resistance gene profiles identified, these MDR isolates may be selected for by the use of macrolides, tetracyclines, and/or in-feed supplements containing heavy metals.
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spelling pubmed-76820202020-11-24 Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016 Klima, Cassidy L. Holman, Devin B. Cook, Shaun R. Conrad, Cheyenne C. Ralston, Brenda J. Allan, Nick Anholt, R. Michele Niu, Yan D. Stanford, Kim Hannon, Sherry J. Booker, Calvin W. McAllister, Tim A. Front Microbiol Microbiology Multidrug-resistant (MDR; resistance to ≥3 antimicrobial classes) members of the Pasteurellaceae family may compromise the efficacy of therapies used to prevent and treat bovine respiratory disease (BRD) in feedlot cattle. This study examined the prevalence of multidrug resistance in strains of Mannheimia haemolytica and Pasteurella multocida collected from BRD cattle mortalities in North America. Isolates of M. haemolytica (n = 147) and P. multocida (n = 70) spanning 69 Alberta feedlots from 2011 to 2016 and two United States feedlots from 2011 to 2012 were examined for antimicrobial resistance (AMR) in association with integrative and conjugative elements (ICEs). Overall, resistance was high in both bacterial species with an increase in the prevalence of MDR isolates between 2011 and 2016. Resistance to >7 antimicrobial drugs occurred in 31% of M. haemolytica and 83% of P. multocida isolates. Resistance to sulfadimethoxine, trimethoprim/sulfamethoxazole, neomycin, clindamycin oxytetracycline, spectinomycin, tylosin, tilmicosin, and tulathromycin was most common. Although >80% of strains harbored three or more ICE-associated genes, only 12% of M. haemolytica and 77% of P. multocida contained all six, reflecting the diversity of ICEs. There was evidence of clonal spread as P. multocida and M. haemolytica isolates with the same pulsed-field gel electrophoresis profile from the United States in 2011 were isolated in Alberta in 2015–2016. This work highlights that MDR strains of Pasteurellaceae containing ICEs are widespread and may be contributing to BRD therapy failure in feedlot cattle. Given the antimicrobial resistance gene profiles identified, these MDR isolates may be selected for by the use of macrolides, tetracyclines, and/or in-feed supplements containing heavy metals. Frontiers Media S.A. 2020-11-05 /pmc/articles/PMC7682020/ /pubmed/33240256 http://dx.doi.org/10.3389/fmicb.2020.606438 Text en Copyright © 2020 Klima, Holman, Cook, Conrad, Ralston, Allan, Anholt, Niu, Stanford, Hannon, Booker, McAllister and Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Klima, Cassidy L.
Holman, Devin B.
Cook, Shaun R.
Conrad, Cheyenne C.
Ralston, Brenda J.
Allan, Nick
Anholt, R. Michele
Niu, Yan D.
Stanford, Kim
Hannon, Sherry J.
Booker, Calvin W.
McAllister, Tim A.
Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016
title Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016
title_full Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016
title_fullStr Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016
title_full_unstemmed Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016
title_short Multidrug Resistance in Pasteurellaceae Associated With Bovine Respiratory Disease Mortalities in North America From 2011 to 2016
title_sort multidrug resistance in pasteurellaceae associated with bovine respiratory disease mortalities in north america from 2011 to 2016
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682020/
https://www.ncbi.nlm.nih.gov/pubmed/33240256
http://dx.doi.org/10.3389/fmicb.2020.606438
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