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An evolutionarily acquired microRNA shapes development of mammalian cortical projections
The corticospinal tract is unique to mammals and the corpus callosum is unique to placental mammals (eutherians). The emergence of these structures is thought to underpin the evolutionary acquisition of complex motor and cognitive skills. Corticospinal motor neurons (CSMN) and callosal projection ne...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682328/ https://www.ncbi.nlm.nih.gov/pubmed/33139574 http://dx.doi.org/10.1073/pnas.2006700117 |
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author | Diaz, Jessica L. Siththanandan, Verl B. Lu, Victoria Gonzalez-Nava, Nicole Pasquina, Lincoln MacDonald, Jessica L. Woodworth, Mollie B. Ozkan, Abdulkadir Nair, Ramesh He, Zihuai Sahni, Vibhu Sarnow, Peter Palmer, Theo D. Macklis, Jeffrey D. Tharin, Suzanne |
author_facet | Diaz, Jessica L. Siththanandan, Verl B. Lu, Victoria Gonzalez-Nava, Nicole Pasquina, Lincoln MacDonald, Jessica L. Woodworth, Mollie B. Ozkan, Abdulkadir Nair, Ramesh He, Zihuai Sahni, Vibhu Sarnow, Peter Palmer, Theo D. Macklis, Jeffrey D. Tharin, Suzanne |
author_sort | Diaz, Jessica L. |
collection | PubMed |
description | The corticospinal tract is unique to mammals and the corpus callosum is unique to placental mammals (eutherians). The emergence of these structures is thought to underpin the evolutionary acquisition of complex motor and cognitive skills. Corticospinal motor neurons (CSMN) and callosal projection neurons (CPN) are the archetypal projection neurons of the corticospinal tract and corpus callosum, respectively. Although a number of conserved transcriptional regulators of CSMN and CPN development have been identified in vertebrates, none are unique to mammals and most are coexpressed across multiple projection neuron subtypes. Here, we discover 17 CSMN-enriched microRNAs (miRNAs), 15 of which map to a single genomic cluster that is exclusive to eutherians. One of these, miR-409-3p, promotes CSMN subtype identity in part via repression of LMO4, a key transcriptional regulator of CPN development. In vivo, miR-409-3p is sufficient to convert deep-layer CPN into CSMN. This is a demonstration of an evolutionarily acquired miRNA in eutherians that refines cortical projection neuron subtype development. Our findings implicate miRNAs in the eutherians’ increase in neuronal subtype and projection diversity, the anatomic underpinnings of their complex behavior. |
format | Online Article Text |
id | pubmed-7682328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-76823282020-12-01 An evolutionarily acquired microRNA shapes development of mammalian cortical projections Diaz, Jessica L. Siththanandan, Verl B. Lu, Victoria Gonzalez-Nava, Nicole Pasquina, Lincoln MacDonald, Jessica L. Woodworth, Mollie B. Ozkan, Abdulkadir Nair, Ramesh He, Zihuai Sahni, Vibhu Sarnow, Peter Palmer, Theo D. Macklis, Jeffrey D. Tharin, Suzanne Proc Natl Acad Sci U S A Biological Sciences The corticospinal tract is unique to mammals and the corpus callosum is unique to placental mammals (eutherians). The emergence of these structures is thought to underpin the evolutionary acquisition of complex motor and cognitive skills. Corticospinal motor neurons (CSMN) and callosal projection neurons (CPN) are the archetypal projection neurons of the corticospinal tract and corpus callosum, respectively. Although a number of conserved transcriptional regulators of CSMN and CPN development have been identified in vertebrates, none are unique to mammals and most are coexpressed across multiple projection neuron subtypes. Here, we discover 17 CSMN-enriched microRNAs (miRNAs), 15 of which map to a single genomic cluster that is exclusive to eutherians. One of these, miR-409-3p, promotes CSMN subtype identity in part via repression of LMO4, a key transcriptional regulator of CPN development. In vivo, miR-409-3p is sufficient to convert deep-layer CPN into CSMN. This is a demonstration of an evolutionarily acquired miRNA in eutherians that refines cortical projection neuron subtype development. Our findings implicate miRNAs in the eutherians’ increase in neuronal subtype and projection diversity, the anatomic underpinnings of their complex behavior. National Academy of Sciences 2020-11-17 2020-11-02 /pmc/articles/PMC7682328/ /pubmed/33139574 http://dx.doi.org/10.1073/pnas.2006700117 Text en Copyright © 2020 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Diaz, Jessica L. Siththanandan, Verl B. Lu, Victoria Gonzalez-Nava, Nicole Pasquina, Lincoln MacDonald, Jessica L. Woodworth, Mollie B. Ozkan, Abdulkadir Nair, Ramesh He, Zihuai Sahni, Vibhu Sarnow, Peter Palmer, Theo D. Macklis, Jeffrey D. Tharin, Suzanne An evolutionarily acquired microRNA shapes development of mammalian cortical projections |
title | An evolutionarily acquired microRNA shapes development of mammalian cortical projections |
title_full | An evolutionarily acquired microRNA shapes development of mammalian cortical projections |
title_fullStr | An evolutionarily acquired microRNA shapes development of mammalian cortical projections |
title_full_unstemmed | An evolutionarily acquired microRNA shapes development of mammalian cortical projections |
title_short | An evolutionarily acquired microRNA shapes development of mammalian cortical projections |
title_sort | evolutionarily acquired microrna shapes development of mammalian cortical projections |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682328/ https://www.ncbi.nlm.nih.gov/pubmed/33139574 http://dx.doi.org/10.1073/pnas.2006700117 |
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