Cargando…

Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis

The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples ob...

Descripción completa

Detalles Bibliográficos
Autores principales: Indenbaum, Victoria, Koren, Ravit, Katz-Likvornik, Shiri, Yitzchaki, Mayan, Halpern, Osnat, Regev-Yochay, Gili, Cohen, Carmit, Biber, Asaf, Feferman, Tali, Cohen Saban, Noy, Dhan, Roni, Levin, Tal, Gozlan, Yael, Weil, Merav, Mor, Orna, Mandelboim, Michal, Sofer, Danit, Mendelson, Ella, Lustig, Yaniv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682882/
https://www.ncbi.nlm.nih.gov/pubmed/33227020
http://dx.doi.org/10.1371/journal.pone.0241164
_version_ 1783612765340958720
author Indenbaum, Victoria
Koren, Ravit
Katz-Likvornik, Shiri
Yitzchaki, Mayan
Halpern, Osnat
Regev-Yochay, Gili
Cohen, Carmit
Biber, Asaf
Feferman, Tali
Cohen Saban, Noy
Dhan, Roni
Levin, Tal
Gozlan, Yael
Weil, Merav
Mor, Orna
Mandelboim, Michal
Sofer, Danit
Mendelson, Ella
Lustig, Yaniv
author_facet Indenbaum, Victoria
Koren, Ravit
Katz-Likvornik, Shiri
Yitzchaki, Mayan
Halpern, Osnat
Regev-Yochay, Gili
Cohen, Carmit
Biber, Asaf
Feferman, Tali
Cohen Saban, Noy
Dhan, Roni
Levin, Tal
Gozlan, Yael
Weil, Merav
Mor, Orna
Mandelboim, Michal
Sofer, Danit
Mendelson, Ella
Lustig, Yaniv
author_sort Indenbaum, Victoria
collection PubMed
description The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4–7 samples each ranging between 1–92 days post first positive PCR were tested by an “in house” ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1–7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35–50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2.
format Online
Article
Text
id pubmed-7682882
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-76828822020-12-02 Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis Indenbaum, Victoria Koren, Ravit Katz-Likvornik, Shiri Yitzchaki, Mayan Halpern, Osnat Regev-Yochay, Gili Cohen, Carmit Biber, Asaf Feferman, Tali Cohen Saban, Noy Dhan, Roni Levin, Tal Gozlan, Yael Weil, Merav Mor, Orna Mandelboim, Michal Sofer, Danit Mendelson, Ella Lustig, Yaniv PLoS One Research Article The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4–7 samples each ranging between 1–92 days post first positive PCR were tested by an “in house” ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1–7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35–50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2. Public Library of Science 2020-11-23 /pmc/articles/PMC7682882/ /pubmed/33227020 http://dx.doi.org/10.1371/journal.pone.0241164 Text en © 2020 Indenbaum et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Indenbaum, Victoria
Koren, Ravit
Katz-Likvornik, Shiri
Yitzchaki, Mayan
Halpern, Osnat
Regev-Yochay, Gili
Cohen, Carmit
Biber, Asaf
Feferman, Tali
Cohen Saban, Noy
Dhan, Roni
Levin, Tal
Gozlan, Yael
Weil, Merav
Mor, Orna
Mandelboim, Michal
Sofer, Danit
Mendelson, Ella
Lustig, Yaniv
Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
title Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
title_full Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
title_fullStr Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
title_full_unstemmed Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
title_short Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
title_sort testing igg antibodies against the rbd of sars-cov-2 is sufficient and necessary for covid-19 diagnosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682882/
https://www.ncbi.nlm.nih.gov/pubmed/33227020
http://dx.doi.org/10.1371/journal.pone.0241164
work_keys_str_mv AT indenbaumvictoria testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT korenravit testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT katzlikvornikshiri testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT yitzchakimayan testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT halpernosnat testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT regevyochaygili testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT cohencarmit testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT biberasaf testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT fefermantali testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT cohensabannoy testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT dhanroni testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT levintal testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT gozlanyael testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT weilmerav testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT mororna testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT mandelboimmichal testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT soferdanit testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT mendelsonella testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis
AT lustigyaniv testingiggantibodiesagainsttherbdofsarscov2issufficientandnecessaryforcovid19diagnosis