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Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis
The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples ob...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682882/ https://www.ncbi.nlm.nih.gov/pubmed/33227020 http://dx.doi.org/10.1371/journal.pone.0241164 |
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author | Indenbaum, Victoria Koren, Ravit Katz-Likvornik, Shiri Yitzchaki, Mayan Halpern, Osnat Regev-Yochay, Gili Cohen, Carmit Biber, Asaf Feferman, Tali Cohen Saban, Noy Dhan, Roni Levin, Tal Gozlan, Yael Weil, Merav Mor, Orna Mandelboim, Michal Sofer, Danit Mendelson, Ella Lustig, Yaniv |
author_facet | Indenbaum, Victoria Koren, Ravit Katz-Likvornik, Shiri Yitzchaki, Mayan Halpern, Osnat Regev-Yochay, Gili Cohen, Carmit Biber, Asaf Feferman, Tali Cohen Saban, Noy Dhan, Roni Levin, Tal Gozlan, Yael Weil, Merav Mor, Orna Mandelboim, Michal Sofer, Danit Mendelson, Ella Lustig, Yaniv |
author_sort | Indenbaum, Victoria |
collection | PubMed |
description | The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4–7 samples each ranging between 1–92 days post first positive PCR were tested by an “in house” ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1–7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35–50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2. |
format | Online Article Text |
id | pubmed-7682882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76828822020-12-02 Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis Indenbaum, Victoria Koren, Ravit Katz-Likvornik, Shiri Yitzchaki, Mayan Halpern, Osnat Regev-Yochay, Gili Cohen, Carmit Biber, Asaf Feferman, Tali Cohen Saban, Noy Dhan, Roni Levin, Tal Gozlan, Yael Weil, Merav Mor, Orna Mandelboim, Michal Sofer, Danit Mendelson, Ella Lustig, Yaniv PLoS One Research Article The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4–7 samples each ranging between 1–92 days post first positive PCR were tested by an “in house” ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1–7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35–50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2. Public Library of Science 2020-11-23 /pmc/articles/PMC7682882/ /pubmed/33227020 http://dx.doi.org/10.1371/journal.pone.0241164 Text en © 2020 Indenbaum et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Indenbaum, Victoria Koren, Ravit Katz-Likvornik, Shiri Yitzchaki, Mayan Halpern, Osnat Regev-Yochay, Gili Cohen, Carmit Biber, Asaf Feferman, Tali Cohen Saban, Noy Dhan, Roni Levin, Tal Gozlan, Yael Weil, Merav Mor, Orna Mandelboim, Michal Sofer, Danit Mendelson, Ella Lustig, Yaniv Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis |
title | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis |
title_full | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis |
title_fullStr | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis |
title_full_unstemmed | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis |
title_short | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis |
title_sort | testing igg antibodies against the rbd of sars-cov-2 is sufficient and necessary for covid-19 diagnosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682882/ https://www.ncbi.nlm.nih.gov/pubmed/33227020 http://dx.doi.org/10.1371/journal.pone.0241164 |
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