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Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing

Pennisetum setaceum ‘Rubrum’ is an ornamental grass plant that produces purple leaves in high-light environments and light purple or green leaves in low-light environments, the latter of which greatly reduces its aesthetic appeal. Therefore, we aimed to identify the key genes associated with leaf co...

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Autores principales: Zhu, Ting, Wang, Xia, Xu, Zhimin, Xu, Jie, Li, Rui, Liu, Ning, Ding, Guochang, Sui, Shunzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682885/
https://www.ncbi.nlm.nih.gov/pubmed/33227025
http://dx.doi.org/10.1371/journal.pone.0242618
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author Zhu, Ting
Wang, Xia
Xu, Zhimin
Xu, Jie
Li, Rui
Liu, Ning
Ding, Guochang
Sui, Shunzhao
author_facet Zhu, Ting
Wang, Xia
Xu, Zhimin
Xu, Jie
Li, Rui
Liu, Ning
Ding, Guochang
Sui, Shunzhao
author_sort Zhu, Ting
collection PubMed
description Pennisetum setaceum ‘Rubrum’ is an ornamental grass plant that produces purple leaves in high-light environments and light purple or green leaves in low-light environments, the latter of which greatly reduces its aesthetic appeal. Therefore, we aimed to identify the key genes associated with leaf coloration and elucidate the molecular mechanisms involved in the color changes in P. setaceum ‘Rubrum’ leaves. We performed transcriptome sequencing of P. setaceum ‘Rubrum’ leaves before and after shading. A total of 19,043 differentially expressed genes were identified, and the numbers of upregulated and downregulated genes at T1 stage, when compared with their expression at the T0 stage, were 10,761 and 8,642, respectively. The possible pathways that determine P. setaceum ‘Rubrum’ leaf color included flavonoid biosynthesis, flavone and flavonol biosynthesis, and carotenoid biosynthesis. There were 31 differentially expressed genes related to chlorophyll metabolism, of which 21 were related to chlorophyll biosynthesis and 10 to chlorophyll degradation, as well as three transcription factors that may be involved in the regulation of chlorophyll degradation. There were 31 key enzyme genes involved in anthocyanin synthesis and accumulation in P. setaceum ‘Rubrum’ leaves, with four transcription factors that may be involved in the regulation of anthocyanin metabolism. The transcriptome data were verified and confirmed reliable by real-time fluorescence quantitative PCR analysis. These findings provide a genetic basis for improving leaf color in P. setaceum ‘Rubrum.’
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spelling pubmed-76828852020-12-02 Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing Zhu, Ting Wang, Xia Xu, Zhimin Xu, Jie Li, Rui Liu, Ning Ding, Guochang Sui, Shunzhao PLoS One Research Article Pennisetum setaceum ‘Rubrum’ is an ornamental grass plant that produces purple leaves in high-light environments and light purple or green leaves in low-light environments, the latter of which greatly reduces its aesthetic appeal. Therefore, we aimed to identify the key genes associated with leaf coloration and elucidate the molecular mechanisms involved in the color changes in P. setaceum ‘Rubrum’ leaves. We performed transcriptome sequencing of P. setaceum ‘Rubrum’ leaves before and after shading. A total of 19,043 differentially expressed genes were identified, and the numbers of upregulated and downregulated genes at T1 stage, when compared with their expression at the T0 stage, were 10,761 and 8,642, respectively. The possible pathways that determine P. setaceum ‘Rubrum’ leaf color included flavonoid biosynthesis, flavone and flavonol biosynthesis, and carotenoid biosynthesis. There were 31 differentially expressed genes related to chlorophyll metabolism, of which 21 were related to chlorophyll biosynthesis and 10 to chlorophyll degradation, as well as three transcription factors that may be involved in the regulation of chlorophyll degradation. There were 31 key enzyme genes involved in anthocyanin synthesis and accumulation in P. setaceum ‘Rubrum’ leaves, with four transcription factors that may be involved in the regulation of anthocyanin metabolism. The transcriptome data were verified and confirmed reliable by real-time fluorescence quantitative PCR analysis. These findings provide a genetic basis for improving leaf color in P. setaceum ‘Rubrum.’ Public Library of Science 2020-11-23 /pmc/articles/PMC7682885/ /pubmed/33227025 http://dx.doi.org/10.1371/journal.pone.0242618 Text en © 2020 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhu, Ting
Wang, Xia
Xu, Zhimin
Xu, Jie
Li, Rui
Liu, Ning
Ding, Guochang
Sui, Shunzhao
Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing
title Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing
title_full Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing
title_fullStr Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing
title_full_unstemmed Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing
title_short Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing
title_sort screening of key genes responsible for pennisetum setaceum ‘rubrum’ leaf color using transcriptome sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682885/
https://www.ncbi.nlm.nih.gov/pubmed/33227025
http://dx.doi.org/10.1371/journal.pone.0242618
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