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Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing
Pennisetum setaceum ‘Rubrum’ is an ornamental grass plant that produces purple leaves in high-light environments and light purple or green leaves in low-light environments, the latter of which greatly reduces its aesthetic appeal. Therefore, we aimed to identify the key genes associated with leaf co...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682885/ https://www.ncbi.nlm.nih.gov/pubmed/33227025 http://dx.doi.org/10.1371/journal.pone.0242618 |
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author | Zhu, Ting Wang, Xia Xu, Zhimin Xu, Jie Li, Rui Liu, Ning Ding, Guochang Sui, Shunzhao |
author_facet | Zhu, Ting Wang, Xia Xu, Zhimin Xu, Jie Li, Rui Liu, Ning Ding, Guochang Sui, Shunzhao |
author_sort | Zhu, Ting |
collection | PubMed |
description | Pennisetum setaceum ‘Rubrum’ is an ornamental grass plant that produces purple leaves in high-light environments and light purple or green leaves in low-light environments, the latter of which greatly reduces its aesthetic appeal. Therefore, we aimed to identify the key genes associated with leaf coloration and elucidate the molecular mechanisms involved in the color changes in P. setaceum ‘Rubrum’ leaves. We performed transcriptome sequencing of P. setaceum ‘Rubrum’ leaves before and after shading. A total of 19,043 differentially expressed genes were identified, and the numbers of upregulated and downregulated genes at T1 stage, when compared with their expression at the T0 stage, were 10,761 and 8,642, respectively. The possible pathways that determine P. setaceum ‘Rubrum’ leaf color included flavonoid biosynthesis, flavone and flavonol biosynthesis, and carotenoid biosynthesis. There were 31 differentially expressed genes related to chlorophyll metabolism, of which 21 were related to chlorophyll biosynthesis and 10 to chlorophyll degradation, as well as three transcription factors that may be involved in the regulation of chlorophyll degradation. There were 31 key enzyme genes involved in anthocyanin synthesis and accumulation in P. setaceum ‘Rubrum’ leaves, with four transcription factors that may be involved in the regulation of anthocyanin metabolism. The transcriptome data were verified and confirmed reliable by real-time fluorescence quantitative PCR analysis. These findings provide a genetic basis for improving leaf color in P. setaceum ‘Rubrum.’ |
format | Online Article Text |
id | pubmed-7682885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76828852020-12-02 Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing Zhu, Ting Wang, Xia Xu, Zhimin Xu, Jie Li, Rui Liu, Ning Ding, Guochang Sui, Shunzhao PLoS One Research Article Pennisetum setaceum ‘Rubrum’ is an ornamental grass plant that produces purple leaves in high-light environments and light purple or green leaves in low-light environments, the latter of which greatly reduces its aesthetic appeal. Therefore, we aimed to identify the key genes associated with leaf coloration and elucidate the molecular mechanisms involved in the color changes in P. setaceum ‘Rubrum’ leaves. We performed transcriptome sequencing of P. setaceum ‘Rubrum’ leaves before and after shading. A total of 19,043 differentially expressed genes were identified, and the numbers of upregulated and downregulated genes at T1 stage, when compared with their expression at the T0 stage, were 10,761 and 8,642, respectively. The possible pathways that determine P. setaceum ‘Rubrum’ leaf color included flavonoid biosynthesis, flavone and flavonol biosynthesis, and carotenoid biosynthesis. There were 31 differentially expressed genes related to chlorophyll metabolism, of which 21 were related to chlorophyll biosynthesis and 10 to chlorophyll degradation, as well as three transcription factors that may be involved in the regulation of chlorophyll degradation. There were 31 key enzyme genes involved in anthocyanin synthesis and accumulation in P. setaceum ‘Rubrum’ leaves, with four transcription factors that may be involved in the regulation of anthocyanin metabolism. The transcriptome data were verified and confirmed reliable by real-time fluorescence quantitative PCR analysis. These findings provide a genetic basis for improving leaf color in P. setaceum ‘Rubrum.’ Public Library of Science 2020-11-23 /pmc/articles/PMC7682885/ /pubmed/33227025 http://dx.doi.org/10.1371/journal.pone.0242618 Text en © 2020 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhu, Ting Wang, Xia Xu, Zhimin Xu, Jie Li, Rui Liu, Ning Ding, Guochang Sui, Shunzhao Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing |
title | Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing |
title_full | Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing |
title_fullStr | Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing |
title_full_unstemmed | Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing |
title_short | Screening of key genes responsible for Pennisetum setaceum ‘Rubrum’ leaf color using transcriptome sequencing |
title_sort | screening of key genes responsible for pennisetum setaceum ‘rubrum’ leaf color using transcriptome sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682885/ https://www.ncbi.nlm.nih.gov/pubmed/33227025 http://dx.doi.org/10.1371/journal.pone.0242618 |
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