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Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei
The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7683347/ https://www.ncbi.nlm.nih.gov/pubmed/33294788 http://dx.doi.org/10.1016/j.isci.2020.101780 |
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author | Rajan, K. Shanmugha Doniger, Tirza Cohen-Chalamish, Smadar Rengaraj, Praveenkumar Galili, Beathrice Aryal, Saurav Unger, Ron Tschudi, Christian Michaeli, Shulamit |
author_facet | Rajan, K. Shanmugha Doniger, Tirza Cohen-Chalamish, Smadar Rengaraj, Praveenkumar Galili, Beathrice Aryal, Saurav Unger, Ron Tschudi, Christian Michaeli, Shulamit |
author_sort | Rajan, K. Shanmugha |
collection | PubMed |
description | The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of trans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact in vivo with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites. |
format | Online Article Text |
id | pubmed-7683347 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-76833472020-12-07 Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei Rajan, K. Shanmugha Doniger, Tirza Cohen-Chalamish, Smadar Rengaraj, Praveenkumar Galili, Beathrice Aryal, Saurav Unger, Ron Tschudi, Christian Michaeli, Shulamit iScience Article The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of trans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact in vivo with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites. Elsevier 2020-11-05 /pmc/articles/PMC7683347/ /pubmed/33294788 http://dx.doi.org/10.1016/j.isci.2020.101780 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Rajan, K. Shanmugha Doniger, Tirza Cohen-Chalamish, Smadar Rengaraj, Praveenkumar Galili, Beathrice Aryal, Saurav Unger, Ron Tschudi, Christian Michaeli, Shulamit Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei |
title | Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei |
title_full | Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei |
title_fullStr | Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei |
title_full_unstemmed | Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei |
title_short | Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosomabrucei |
title_sort | developmentally regulated novel non-coding anti-sense regulators of mrna translation in trypanosomabrucei |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7683347/ https://www.ncbi.nlm.nih.gov/pubmed/33294788 http://dx.doi.org/10.1016/j.isci.2020.101780 |
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