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Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility

The mechanism of protection against cholera afforded by previous illness or vaccination is currently unknown. We have recently shown that antibodies targeting O-specific polysaccharide (OSP) of Vibrio cholerae correlate highly with protection against cholera. V. cholerae is highly motile and possess...

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Autores principales: Charles, Richelle C., Kelly, Meagan, Tam, Jenny M., Akter, Aklima, Hossain, Motaher, Islam, Kamrul, Biswas, Rajib, Kamruzzaman, Mohammad, Chowdhury, Fahima, Khan, Ashraful I., Leung, Daniel T., Weil, Ana, LaRocque, Regina C., Bhuiyan, Taufiqur Rahman, Rahman, Atiqur, Mayo-Smith, Leslie M., Becker, Rachel L., Vyas, Jatin M., Faherty, Christina S., Nickerson, Kourtney P., Giffen, Samantha, Ritter, Alaina S., Waldor, Matthew K., Xu, Peng, Kováč, Pavol, Calderwood, Stephen B., Kauffman, Robert C., Wrammert, Jens, Qadri, Firdausi, Harris, Jason B., Ryan, Edward T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7683404/
https://www.ncbi.nlm.nih.gov/pubmed/33203761
http://dx.doi.org/10.1128/mBio.02847-20
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author Charles, Richelle C.
Kelly, Meagan
Tam, Jenny M.
Akter, Aklima
Hossain, Motaher
Islam, Kamrul
Biswas, Rajib
Kamruzzaman, Mohammad
Chowdhury, Fahima
Khan, Ashraful I.
Leung, Daniel T.
Weil, Ana
LaRocque, Regina C.
Bhuiyan, Taufiqur Rahman
Rahman, Atiqur
Mayo-Smith, Leslie M.
Becker, Rachel L.
Vyas, Jatin M.
Faherty, Christina S.
Nickerson, Kourtney P.
Giffen, Samantha
Ritter, Alaina S.
Waldor, Matthew K.
Xu, Peng
Kováč, Pavol
Calderwood, Stephen B.
Kauffman, Robert C.
Wrammert, Jens
Qadri, Firdausi
Harris, Jason B.
Ryan, Edward T.
author_facet Charles, Richelle C.
Kelly, Meagan
Tam, Jenny M.
Akter, Aklima
Hossain, Motaher
Islam, Kamrul
Biswas, Rajib
Kamruzzaman, Mohammad
Chowdhury, Fahima
Khan, Ashraful I.
Leung, Daniel T.
Weil, Ana
LaRocque, Regina C.
Bhuiyan, Taufiqur Rahman
Rahman, Atiqur
Mayo-Smith, Leslie M.
Becker, Rachel L.
Vyas, Jatin M.
Faherty, Christina S.
Nickerson, Kourtney P.
Giffen, Samantha
Ritter, Alaina S.
Waldor, Matthew K.
Xu, Peng
Kováč, Pavol
Calderwood, Stephen B.
Kauffman, Robert C.
Wrammert, Jens
Qadri, Firdausi
Harris, Jason B.
Ryan, Edward T.
author_sort Charles, Richelle C.
collection PubMed
description The mechanism of protection against cholera afforded by previous illness or vaccination is currently unknown. We have recently shown that antibodies targeting O-specific polysaccharide (OSP) of Vibrio cholerae correlate highly with protection against cholera. V. cholerae is highly motile and possesses a flagellum sheathed in OSP, and motility of V. cholerae correlates with virulence. Using high-speed video microscopy and building upon previous animal-related work, we demonstrate that sera, polyclonal antibody fractions, and OSP-specific monoclonal antibodies recovered from humans surviving cholera block V. cholerae motility at both subagglutinating and agglutinating concentrations. This antimotility effect is reversed by preadsorbing sera and polyclonal antibody fractions with purified OSP and is associated with OSP-specific but not flagellin-specific monoclonal antibodies. Fab fragments of OSP-specific polyclonal antibodies do not inhibit motility, suggesting a requirement for antibody-mediated cross-linking in motility inhibition. We show that OSP-specific antibodies do not directly affect V. cholerae viability, but that OSP-specific monoclonal antibody highly protects against death in the murine cholera model. We used in vivo competitive index studies to demonstrate that OSP-specific antibodies impede colonization and survival of V. cholerae in intestinal tissues and that this impact is motility dependent. Our findings suggest that the impedance of motility by antibodies targeting V. cholerae OSP contributes to protection against cholera.
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spelling pubmed-76834042020-11-30 Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility Charles, Richelle C. Kelly, Meagan Tam, Jenny M. Akter, Aklima Hossain, Motaher Islam, Kamrul Biswas, Rajib Kamruzzaman, Mohammad Chowdhury, Fahima Khan, Ashraful I. Leung, Daniel T. Weil, Ana LaRocque, Regina C. Bhuiyan, Taufiqur Rahman Rahman, Atiqur Mayo-Smith, Leslie M. Becker, Rachel L. Vyas, Jatin M. Faherty, Christina S. Nickerson, Kourtney P. Giffen, Samantha Ritter, Alaina S. Waldor, Matthew K. Xu, Peng Kováč, Pavol Calderwood, Stephen B. Kauffman, Robert C. Wrammert, Jens Qadri, Firdausi Harris, Jason B. Ryan, Edward T. mBio Research Article The mechanism of protection against cholera afforded by previous illness or vaccination is currently unknown. We have recently shown that antibodies targeting O-specific polysaccharide (OSP) of Vibrio cholerae correlate highly with protection against cholera. V. cholerae is highly motile and possesses a flagellum sheathed in OSP, and motility of V. cholerae correlates with virulence. Using high-speed video microscopy and building upon previous animal-related work, we demonstrate that sera, polyclonal antibody fractions, and OSP-specific monoclonal antibodies recovered from humans surviving cholera block V. cholerae motility at both subagglutinating and agglutinating concentrations. This antimotility effect is reversed by preadsorbing sera and polyclonal antibody fractions with purified OSP and is associated with OSP-specific but not flagellin-specific monoclonal antibodies. Fab fragments of OSP-specific polyclonal antibodies do not inhibit motility, suggesting a requirement for antibody-mediated cross-linking in motility inhibition. We show that OSP-specific antibodies do not directly affect V. cholerae viability, but that OSP-specific monoclonal antibody highly protects against death in the murine cholera model. We used in vivo competitive index studies to demonstrate that OSP-specific antibodies impede colonization and survival of V. cholerae in intestinal tissues and that this impact is motility dependent. Our findings suggest that the impedance of motility by antibodies targeting V. cholerae OSP contributes to protection against cholera. American Society for Microbiology 2020-11-17 /pmc/articles/PMC7683404/ /pubmed/33203761 http://dx.doi.org/10.1128/mBio.02847-20 Text en Copyright © 2020 Charles et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Charles, Richelle C.
Kelly, Meagan
Tam, Jenny M.
Akter, Aklima
Hossain, Motaher
Islam, Kamrul
Biswas, Rajib
Kamruzzaman, Mohammad
Chowdhury, Fahima
Khan, Ashraful I.
Leung, Daniel T.
Weil, Ana
LaRocque, Regina C.
Bhuiyan, Taufiqur Rahman
Rahman, Atiqur
Mayo-Smith, Leslie M.
Becker, Rachel L.
Vyas, Jatin M.
Faherty, Christina S.
Nickerson, Kourtney P.
Giffen, Samantha
Ritter, Alaina S.
Waldor, Matthew K.
Xu, Peng
Kováč, Pavol
Calderwood, Stephen B.
Kauffman, Robert C.
Wrammert, Jens
Qadri, Firdausi
Harris, Jason B.
Ryan, Edward T.
Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility
title Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility
title_full Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility
title_fullStr Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility
title_full_unstemmed Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility
title_short Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility
title_sort humans surviving cholera develop antibodies against vibrio cholerae o-specific polysaccharide that inhibit pathogen motility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7683404/
https://www.ncbi.nlm.nih.gov/pubmed/33203761
http://dx.doi.org/10.1128/mBio.02847-20
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