Cargando…
Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus
Six new triterpene saponins (1–5,7) and 3 known saponins (6,8,9) were isolated from MeOH extracts of the cactus Stenocereus pruinosus. The structures of the isolated saponins were elucidated using MS, IR, and comprehensive NMR measurements. To develop drugs for treating Alzheimer’s disease (AD) on t...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684148/ https://www.ncbi.nlm.nih.gov/pubmed/33231837 http://dx.doi.org/10.1007/s11418-020-01463-0 |
_version_ | 1783612993442938880 |
---|---|
author | Fujihara, Koji Shimoyama, Takuya Kawazu, Ryo Sasaki, Hiroaki Koyama, Kiyotaka Takahashi, Kunio Kinoshita, Kaoru |
author_facet | Fujihara, Koji Shimoyama, Takuya Kawazu, Ryo Sasaki, Hiroaki Koyama, Kiyotaka Takahashi, Kunio Kinoshita, Kaoru |
author_sort | Fujihara, Koji |
collection | PubMed |
description | Six new triterpene saponins (1–5,7) and 3 known saponins (6,8,9) were isolated from MeOH extracts of the cactus Stenocereus pruinosus. The structures of the isolated saponins were elucidated using MS, IR, and comprehensive NMR measurements. To develop drugs for treating Alzheimer’s disease (AD) on the basis of the amyloid cascade hypothesis, the isolated saponins were evaluated for inhibition of BACE1 activity and amyloid beta (Aβ) aggregation using thioflavin-T assay, and triterpenes as an aglycone moiety and an alkaline hydrolysate of the saponins were also evaluated. One saponin, stenoside A (7), exhibited inhibitory activity related to Aβ aggregation and its degree of Aβ aggregation was 40.6% at 100 μM. GRAPHIC ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11418-020-01463-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7684148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-76841482020-11-24 Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus Fujihara, Koji Shimoyama, Takuya Kawazu, Ryo Sasaki, Hiroaki Koyama, Kiyotaka Takahashi, Kunio Kinoshita, Kaoru J Nat Med Original Paper Six new triterpene saponins (1–5,7) and 3 known saponins (6,8,9) were isolated from MeOH extracts of the cactus Stenocereus pruinosus. The structures of the isolated saponins were elucidated using MS, IR, and comprehensive NMR measurements. To develop drugs for treating Alzheimer’s disease (AD) on the basis of the amyloid cascade hypothesis, the isolated saponins were evaluated for inhibition of BACE1 activity and amyloid beta (Aβ) aggregation using thioflavin-T assay, and triterpenes as an aglycone moiety and an alkaline hydrolysate of the saponins were also evaluated. One saponin, stenoside A (7), exhibited inhibitory activity related to Aβ aggregation and its degree of Aβ aggregation was 40.6% at 100 μM. GRAPHIC ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11418-020-01463-0) contains supplementary material, which is available to authorized users. Springer Singapore 2020-11-24 2021 /pmc/articles/PMC7684148/ /pubmed/33231837 http://dx.doi.org/10.1007/s11418-020-01463-0 Text en © The Japanese Society of Pharmacognosy 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Paper Fujihara, Koji Shimoyama, Takuya Kawazu, Ryo Sasaki, Hiroaki Koyama, Kiyotaka Takahashi, Kunio Kinoshita, Kaoru Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus |
title | Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus |
title_full | Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus |
title_fullStr | Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus |
title_full_unstemmed | Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus |
title_short | Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus |
title_sort | amyloid β aggregation inhibitory activity of triterpene saponins from the cactus stenocereus pruinosus |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684148/ https://www.ncbi.nlm.nih.gov/pubmed/33231837 http://dx.doi.org/10.1007/s11418-020-01463-0 |
work_keys_str_mv | AT fujiharakoji amyloidbaggregationinhibitoryactivityoftriterpenesaponinsfromthecactusstenocereuspruinosus AT shimoyamatakuya amyloidbaggregationinhibitoryactivityoftriterpenesaponinsfromthecactusstenocereuspruinosus AT kawazuryo amyloidbaggregationinhibitoryactivityoftriterpenesaponinsfromthecactusstenocereuspruinosus AT sasakihiroaki amyloidbaggregationinhibitoryactivityoftriterpenesaponinsfromthecactusstenocereuspruinosus AT koyamakiyotaka amyloidbaggregationinhibitoryactivityoftriterpenesaponinsfromthecactusstenocereuspruinosus AT takahashikunio amyloidbaggregationinhibitoryactivityoftriterpenesaponinsfromthecactusstenocereuspruinosus AT kinoshitakaoru amyloidbaggregationinhibitoryactivityoftriterpenesaponinsfromthecactusstenocereuspruinosus |