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A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge
Schmallenberg virus (SBV), an arthropod-transmitted pathogenic bunyavirus, continues to be a threat to the European livestock industry, causing morbidity and mortality among young ruminant livestock. Here, we describe a novel SBV subunit vaccine, based on bacterially expressed SBV nucleoprotein (SBV...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684302/ https://www.ncbi.nlm.nih.gov/pubmed/33230115 http://dx.doi.org/10.1038/s41598-020-73424-2 |
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author | Boshra, Hani Lorenzo, Gema Charro, Diego Moreno, Sandra Guerra, Gabriel Soares Sanchez, Isbene Garrido, Joseba M. Geijo, Marivi Brun, Alejandro Abrescia, Nicola G. A. |
author_facet | Boshra, Hani Lorenzo, Gema Charro, Diego Moreno, Sandra Guerra, Gabriel Soares Sanchez, Isbene Garrido, Joseba M. Geijo, Marivi Brun, Alejandro Abrescia, Nicola G. A. |
author_sort | Boshra, Hani |
collection | PubMed |
description | Schmallenberg virus (SBV), an arthropod-transmitted pathogenic bunyavirus, continues to be a threat to the European livestock industry, causing morbidity and mortality among young ruminant livestock. Here, we describe a novel SBV subunit vaccine, based on bacterially expressed SBV nucleoprotein (SBV-N) administered with a veterinary-grade Saponin adjuvant. When assayed in an IFNAR(-/-) mouse model, SBV-N with Saponin induced strong non-neutralizing broadly virus-reactive antibodies, decreased clinical signs, as well as significantly reduced viremia. Vaccination assays also suggest that this level of immune protection is cell mediated, as evidenced by the lack of neutralizing antibodies, as well as interferon-γ secretion observed in vitro. Therefore, based on these results, bacterially expressed SBV-N, co-administered with veterinary-grade Saponin adjuvant may serve as a promising economical alternative to current SBV vaccines, and warrant further evaluation in large ruminant animal models. Moreover, we propose that this strategy may be applicable to other bunyaviruses. |
format | Online Article Text |
id | pubmed-7684302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76843022020-11-27 A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge Boshra, Hani Lorenzo, Gema Charro, Diego Moreno, Sandra Guerra, Gabriel Soares Sanchez, Isbene Garrido, Joseba M. Geijo, Marivi Brun, Alejandro Abrescia, Nicola G. A. Sci Rep Article Schmallenberg virus (SBV), an arthropod-transmitted pathogenic bunyavirus, continues to be a threat to the European livestock industry, causing morbidity and mortality among young ruminant livestock. Here, we describe a novel SBV subunit vaccine, based on bacterially expressed SBV nucleoprotein (SBV-N) administered with a veterinary-grade Saponin adjuvant. When assayed in an IFNAR(-/-) mouse model, SBV-N with Saponin induced strong non-neutralizing broadly virus-reactive antibodies, decreased clinical signs, as well as significantly reduced viremia. Vaccination assays also suggest that this level of immune protection is cell mediated, as evidenced by the lack of neutralizing antibodies, as well as interferon-γ secretion observed in vitro. Therefore, based on these results, bacterially expressed SBV-N, co-administered with veterinary-grade Saponin adjuvant may serve as a promising economical alternative to current SBV vaccines, and warrant further evaluation in large ruminant animal models. Moreover, we propose that this strategy may be applicable to other bunyaviruses. Nature Publishing Group UK 2020-11-23 /pmc/articles/PMC7684302/ /pubmed/33230115 http://dx.doi.org/10.1038/s41598-020-73424-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Boshra, Hani Lorenzo, Gema Charro, Diego Moreno, Sandra Guerra, Gabriel Soares Sanchez, Isbene Garrido, Joseba M. Geijo, Marivi Brun, Alejandro Abrescia, Nicola G. A. A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge |
title | A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge |
title_full | A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge |
title_fullStr | A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge |
title_full_unstemmed | A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge |
title_short | A novel Schmallenberg virus subunit vaccine candidate protects IFNAR(-/-) mice against virulent SBV challenge |
title_sort | novel schmallenberg virus subunit vaccine candidate protects ifnar(-/-) mice against virulent sbv challenge |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684302/ https://www.ncbi.nlm.nih.gov/pubmed/33230115 http://dx.doi.org/10.1038/s41598-020-73424-2 |
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