Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis

[Image: see text] The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask...

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Autores principales: Haas, Thomas M., Qiu, Danye, Häner, Markus, Angebauer, Larissa, Ripp, Alexander, Singh, Jyoti, Koch, Hans-Georg, Jessen-Trefzer, Claudia, Jessen, Henning J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684580/
https://www.ncbi.nlm.nih.gov/pubmed/32502348
http://dx.doi.org/10.1021/acs.joc.0c00841
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author Haas, Thomas M.
Qiu, Danye
Häner, Markus
Angebauer, Larissa
Ripp, Alexander
Singh, Jyoti
Koch, Hans-Georg
Jessen-Trefzer, Claudia
Jessen, Henning J.
author_facet Haas, Thomas M.
Qiu, Danye
Häner, Markus
Angebauer, Larissa
Ripp, Alexander
Singh, Jyoti
Koch, Hans-Georg
Jessen-Trefzer, Claudia
Jessen, Henning J.
author_sort Haas, Thomas M.
collection PubMed
description [Image: see text] The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.
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spelling pubmed-76845802020-11-25 Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis Haas, Thomas M. Qiu, Danye Häner, Markus Angebauer, Larissa Ripp, Alexander Singh, Jyoti Koch, Hans-Georg Jessen-Trefzer, Claudia Jessen, Henning J. J Org Chem [Image: see text] The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts. American Chemical Society 2020-06-05 2020-11-20 /pmc/articles/PMC7684580/ /pubmed/32502348 http://dx.doi.org/10.1021/acs.joc.0c00841 Text en This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Haas, Thomas M.
Qiu, Danye
Häner, Markus
Angebauer, Larissa
Ripp, Alexander
Singh, Jyoti
Koch, Hans-Georg
Jessen-Trefzer, Claudia
Jessen, Henning J.
Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
title Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
title_full Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
title_fullStr Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
title_full_unstemmed Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
title_short Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
title_sort four phosphates at one blow: access to pentaphosphorylated magic spot nucleotides and their analysis by capillary electrophoresis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684580/
https://www.ncbi.nlm.nih.gov/pubmed/32502348
http://dx.doi.org/10.1021/acs.joc.0c00841
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