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The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC

Both E‐ and Z‐isomers of lycopene are encountered in nature. Although they were separated on C30‐HPLC by the mobile phase consisting of CH(3)CN‐MeOH (A) and MTBE (B), the quantification of Z‐isomers cannot be archived at present because either their commercially available reference samples are curre...

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Autores principales: Huang, Jin, Hui, Bodi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684632/
https://www.ncbi.nlm.nih.gov/pubmed/33282246
http://dx.doi.org/10.1002/fsn3.1879
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author Huang, Jin
Hui, Bodi
author_facet Huang, Jin
Hui, Bodi
author_sort Huang, Jin
collection PubMed
description Both E‐ and Z‐isomers of lycopene are encountered in nature. Although they were separated on C30‐HPLC by the mobile phase consisting of CH(3)CN‐MeOH (A) and MTBE (B), the quantification of Z‐isomers cannot be archived at present because either their commercially available reference samples are currently lacking, or they are unstable. In this study, both the specific and molar absorption coefficients of 5, 9, and 13 Z‐isomers in the mobile phase were determined on the analytical C30‐HPLC‐PDA‐ELSD, and further verified on the preparative C30‐HPLC‐PDA. The specific and molar absorption coefficients of 5, 9, and 13 Z‐isomers were finally verified to be [Formula: see text]  = 3,422 and ε (mol) = 183,717, [Formula: see text]  = 2,183 and ε (mol) = 117,199, and [Formula: see text]  = 1,119 and ε (mol) = 60,076, respectively, in the mobile phase. With these determined coefficients, the quantifications of 5, 9, and 13 Z‐isomers were able to be archived on C30‐HPLC‐PDA with the mobile phase applied in this investigation.
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spelling pubmed-76846322020-12-03 The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC Huang, Jin Hui, Bodi Food Sci Nutr Original Research Both E‐ and Z‐isomers of lycopene are encountered in nature. Although they were separated on C30‐HPLC by the mobile phase consisting of CH(3)CN‐MeOH (A) and MTBE (B), the quantification of Z‐isomers cannot be archived at present because either their commercially available reference samples are currently lacking, or they are unstable. In this study, both the specific and molar absorption coefficients of 5, 9, and 13 Z‐isomers in the mobile phase were determined on the analytical C30‐HPLC‐PDA‐ELSD, and further verified on the preparative C30‐HPLC‐PDA. The specific and molar absorption coefficients of 5, 9, and 13 Z‐isomers were finally verified to be [Formula: see text]  = 3,422 and ε (mol) = 183,717, [Formula: see text]  = 2,183 and ε (mol) = 117,199, and [Formula: see text]  = 1,119 and ε (mol) = 60,076, respectively, in the mobile phase. With these determined coefficients, the quantifications of 5, 9, and 13 Z‐isomers were able to be archived on C30‐HPLC‐PDA with the mobile phase applied in this investigation. John Wiley and Sons Inc. 2020-10-13 /pmc/articles/PMC7684632/ /pubmed/33282246 http://dx.doi.org/10.1002/fsn3.1879 Text en © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Huang, Jin
Hui, Bodi
The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC
title The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC
title_full The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC
title_fullStr The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC
title_full_unstemmed The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC
title_short The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC
title_sort determination of lycopene z‐isomer absorption coefficient on c30‐hplc
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684632/
https://www.ncbi.nlm.nih.gov/pubmed/33282246
http://dx.doi.org/10.1002/fsn3.1879
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