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A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures

BACKGROUND: CRISPR‐Cas9‐based technologies have revolutionized experimental manipulation of mammalian genomes. None‐the‐less, limitations of the delivery and efficacy of these technologies restrict their application in primary cells. AIMS: To create an optimized protocol for penetrant, reproducible,...

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Detalles Bibliográficos
Autores principales:	Hoellerbauer, Pia, Kufeld, Megan, Arora, Sonali, Wu, Hua‐Jun, Feldman, Heather M., Paddison, Patrick J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2020
Materias:	Method Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685144/ https://www.ncbi.nlm.nih.gov/pubmed/32721120 http://dx.doi.org/10.1002/cnr2.1269

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