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Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells
BACKGROUND: Goose parvoviruses (GPVs) spread globally and cause a huge economic loss to the poultry industry. Although the attenuated GPV vaccines play a key role in preventing the disease caused by GPV, the molecular basis for the attenuation of GPV is barely known. RESULTS: A highly attenuated GPV...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685651/ https://www.ncbi.nlm.nih.gov/pubmed/33228649 http://dx.doi.org/10.1186/s12917-020-02673-0 |
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author | Shao, Hongxia Jiang, Yuchen Yuan, Huisha Ji, Lifei Jin, Wenjie Qian, Kun Ye, Jianqiang Qin, Aijian |
author_facet | Shao, Hongxia Jiang, Yuchen Yuan, Huisha Ji, Lifei Jin, Wenjie Qian, Kun Ye, Jianqiang Qin, Aijian |
author_sort | Shao, Hongxia |
collection | PubMed |
description | BACKGROUND: Goose parvoviruses (GPVs) spread globally and cause a huge economic loss to the poultry industry. Although the attenuated GPV vaccines play a key role in preventing the disease caused by GPV, the molecular basis for the attenuation of GPV is barely known. RESULTS: A highly attenuated GPV strain, GPV-CZM-142, was generated through blindly passaging of the highly pathogenic strain, GPV-CZM, in goose embryonic fibroblasts (GEF) for 142 generations. The GEF-adapted GPV strain’s virulence was 10,000 times weaker than its wild type counterpart, GPV-CZM, based on the ELD(50) (50% Embryo Lethal Dose). By comparing with the wild type strain, genome sequencing analysis identified adapted mutations either in ITR or in NS and VP1 of GPV-CZM-142. CONCLUSIONS: The highly attenuated GPV strain, GPV-CZM-142, provides a GPV vaccine candidate, and the identified virulence-related mutations give a novel insight into the molecular determinants of GPV virulence. |
format | Online Article Text |
id | pubmed-7685651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-76856512020-11-25 Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells Shao, Hongxia Jiang, Yuchen Yuan, Huisha Ji, Lifei Jin, Wenjie Qian, Kun Ye, Jianqiang Qin, Aijian BMC Vet Res Research Article BACKGROUND: Goose parvoviruses (GPVs) spread globally and cause a huge economic loss to the poultry industry. Although the attenuated GPV vaccines play a key role in preventing the disease caused by GPV, the molecular basis for the attenuation of GPV is barely known. RESULTS: A highly attenuated GPV strain, GPV-CZM-142, was generated through blindly passaging of the highly pathogenic strain, GPV-CZM, in goose embryonic fibroblasts (GEF) for 142 generations. The GEF-adapted GPV strain’s virulence was 10,000 times weaker than its wild type counterpart, GPV-CZM, based on the ELD(50) (50% Embryo Lethal Dose). By comparing with the wild type strain, genome sequencing analysis identified adapted mutations either in ITR or in NS and VP1 of GPV-CZM-142. CONCLUSIONS: The highly attenuated GPV strain, GPV-CZM-142, provides a GPV vaccine candidate, and the identified virulence-related mutations give a novel insight into the molecular determinants of GPV virulence. BioMed Central 2020-11-23 /pmc/articles/PMC7685651/ /pubmed/33228649 http://dx.doi.org/10.1186/s12917-020-02673-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Shao, Hongxia Jiang, Yuchen Yuan, Huisha Ji, Lifei Jin, Wenjie Qian, Kun Ye, Jianqiang Qin, Aijian Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells |
title | Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells |
title_full | Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells |
title_fullStr | Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells |
title_full_unstemmed | Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells |
title_short | Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells |
title_sort | generation and molecular characteristics of a highly attenuated gpv strain through adaptation in gef cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685651/ https://www.ncbi.nlm.nih.gov/pubmed/33228649 http://dx.doi.org/10.1186/s12917-020-02673-0 |
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