Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection

Due to global shortages of flocked nasopharyngeal swabs and appropriate viral transport media during the COVID-19 pandemic, alternate diagnostic specimens for SARS-CoV-2 detection are sought. The accuracy and feasibility of saliva samples collected and transported without specialized collection devi...

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Autores principales: Matic, Nancy, Stefanovic, Aleksandra, Leung, Victor, Lawson, Tanya, Ritchie, Gordon, Li, Lynne, Champagne, Sylvie, Romney, Marc G., Lowe, Christopher F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685775/
https://www.ncbi.nlm.nih.gov/pubmed/33236269
http://dx.doi.org/10.1007/s10096-020-04090-5
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author Matic, Nancy
Stefanovic, Aleksandra
Leung, Victor
Lawson, Tanya
Ritchie, Gordon
Li, Lynne
Champagne, Sylvie
Romney, Marc G.
Lowe, Christopher F.
author_facet Matic, Nancy
Stefanovic, Aleksandra
Leung, Victor
Lawson, Tanya
Ritchie, Gordon
Li, Lynne
Champagne, Sylvie
Romney, Marc G.
Lowe, Christopher F.
author_sort Matic, Nancy
collection PubMed
description Due to global shortages of flocked nasopharyngeal swabs and appropriate viral transport media during the COVID-19 pandemic, alternate diagnostic specimens for SARS-CoV-2 detection are sought. The accuracy and feasibility of saliva samples collected and transported without specialized collection devices or media were evaluated. Saliva demonstrated good concordance with paired nasopharyngeal swabs for SARS-CoV-2 detection in 67/74 cases (90.5%), though barriers to saliva collection were observed in long-term care residents and outbreak settings. SARS-CoV-2 RNA was stable in human saliva at room temperature for up to 48 h after initial specimen collection, informing appropriate transport time and conditions.
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spelling pubmed-76857752020-11-25 Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection Matic, Nancy Stefanovic, Aleksandra Leung, Victor Lawson, Tanya Ritchie, Gordon Li, Lynne Champagne, Sylvie Romney, Marc G. Lowe, Christopher F. Eur J Clin Microbiol Infect Dis Brief Report Due to global shortages of flocked nasopharyngeal swabs and appropriate viral transport media during the COVID-19 pandemic, alternate diagnostic specimens for SARS-CoV-2 detection are sought. The accuracy and feasibility of saliva samples collected and transported without specialized collection devices or media were evaluated. Saliva demonstrated good concordance with paired nasopharyngeal swabs for SARS-CoV-2 detection in 67/74 cases (90.5%), though barriers to saliva collection were observed in long-term care residents and outbreak settings. SARS-CoV-2 RNA was stable in human saliva at room temperature for up to 48 h after initial specimen collection, informing appropriate transport time and conditions. Springer Berlin Heidelberg 2020-11-25 2021 /pmc/articles/PMC7685775/ /pubmed/33236269 http://dx.doi.org/10.1007/s10096-020-04090-5 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Matic, Nancy
Stefanovic, Aleksandra
Leung, Victor
Lawson, Tanya
Ritchie, Gordon
Li, Lynne
Champagne, Sylvie
Romney, Marc G.
Lowe, Christopher F.
Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
title Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
title_full Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
title_fullStr Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
title_full_unstemmed Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
title_short Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
title_sort practical challenges to the clinical implementation of saliva for sars-cov-2 detection
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685775/
https://www.ncbi.nlm.nih.gov/pubmed/33236269
http://dx.doi.org/10.1007/s10096-020-04090-5
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