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Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages

Escherichia coli is the leading cause of urinary tract infection, one of the most common bacterial infections in humans. Despite this, a genomic perspective is lacking regarding the phylogenetic distribution of isolates associated with different clinical syndromes. Here, we present a large-scale phy...

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Autores principales: Biggel, Michael, Xavier, Basil B., Johnson, James R., Nielsen, Karen L., Frimodt-Møller, Niels, Matheeussen, Veerle, Goossens, Herman, Moons, Pieter, Van Puyvelde, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686366/
https://www.ncbi.nlm.nih.gov/pubmed/33235212
http://dx.doi.org/10.1038/s41467-020-19714-9
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author Biggel, Michael
Xavier, Basil B.
Johnson, James R.
Nielsen, Karen L.
Frimodt-Møller, Niels
Matheeussen, Veerle
Goossens, Herman
Moons, Pieter
Van Puyvelde, Sandra
author_facet Biggel, Michael
Xavier, Basil B.
Johnson, James R.
Nielsen, Karen L.
Frimodt-Møller, Niels
Matheeussen, Veerle
Goossens, Herman
Moons, Pieter
Van Puyvelde, Sandra
author_sort Biggel, Michael
collection PubMed
description Escherichia coli is the leading cause of urinary tract infection, one of the most common bacterial infections in humans. Despite this, a genomic perspective is lacking regarding the phylogenetic distribution of isolates associated with different clinical syndromes. Here, we present a large-scale phylogenomic analysis of a spatiotemporally and clinically diverse set of 907 E. coli isolates, including 722 uropathogenic E. coli (UPEC) isolates. A genome-wide association approach identifies the (P-fimbriae-encoding) papGII locus as the key feature distinguishing invasive UPEC, defined as isolates associated with severe UTI, i.e., kidney infection (pyelonephritis) or urinary-source bacteremia, from non-invasive UPEC, defined as isolates associated with asymptomatic bacteriuria or bladder infection (cystitis). Within the E. coli population, distinct invasive UPEC lineages emerged through repeated horizontal acquisition of diverse papGII-containing pathogenicity islands. Our findings elucidate the molecular determinants of severe UTI and have implications for the early detection of this pathogen.
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spelling pubmed-76863662020-12-03 Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages Biggel, Michael Xavier, Basil B. Johnson, James R. Nielsen, Karen L. Frimodt-Møller, Niels Matheeussen, Veerle Goossens, Herman Moons, Pieter Van Puyvelde, Sandra Nat Commun Article Escherichia coli is the leading cause of urinary tract infection, one of the most common bacterial infections in humans. Despite this, a genomic perspective is lacking regarding the phylogenetic distribution of isolates associated with different clinical syndromes. Here, we present a large-scale phylogenomic analysis of a spatiotemporally and clinically diverse set of 907 E. coli isolates, including 722 uropathogenic E. coli (UPEC) isolates. A genome-wide association approach identifies the (P-fimbriae-encoding) papGII locus as the key feature distinguishing invasive UPEC, defined as isolates associated with severe UTI, i.e., kidney infection (pyelonephritis) or urinary-source bacteremia, from non-invasive UPEC, defined as isolates associated with asymptomatic bacteriuria or bladder infection (cystitis). Within the E. coli population, distinct invasive UPEC lineages emerged through repeated horizontal acquisition of diverse papGII-containing pathogenicity islands. Our findings elucidate the molecular determinants of severe UTI and have implications for the early detection of this pathogen. Nature Publishing Group UK 2020-11-24 /pmc/articles/PMC7686366/ /pubmed/33235212 http://dx.doi.org/10.1038/s41467-020-19714-9 Text en © Crown 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Biggel, Michael
Xavier, Basil B.
Johnson, James R.
Nielsen, Karen L.
Frimodt-Møller, Niels
Matheeussen, Veerle
Goossens, Herman
Moons, Pieter
Van Puyvelde, Sandra
Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages
title Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages
title_full Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages
title_fullStr Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages
title_full_unstemmed Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages
title_short Horizontally acquired papGII-containing pathogenicity islands underlie the emergence of invasive uropathogenic Escherichia coli lineages
title_sort horizontally acquired papgii-containing pathogenicity islands underlie the emergence of invasive uropathogenic escherichia coli lineages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686366/
https://www.ncbi.nlm.nih.gov/pubmed/33235212
http://dx.doi.org/10.1038/s41467-020-19714-9
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