Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing

Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) te...

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Autores principales: Moldován, Norbert, Torma, Gábor, Gulyás, Gábor, Hornyák, Ákos, Zádori, Zoltán, Jefferson, Victoria A., Csabai, Zsolt, Boldogkői, Miklós, Tombácz, Dóra, Meyer, Florencia, Boldogkői, Zsolt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686369/
https://www.ncbi.nlm.nih.gov/pubmed/33235226
http://dx.doi.org/10.1038/s41598-020-77520-1
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author Moldován, Norbert
Torma, Gábor
Gulyás, Gábor
Hornyák, Ákos
Zádori, Zoltán
Jefferson, Victoria A.
Csabai, Zsolt
Boldogkői, Miklós
Tombácz, Dóra
Meyer, Florencia
Boldogkői, Zsolt
author_facet Moldován, Norbert
Torma, Gábor
Gulyás, Gábor
Hornyák, Ákos
Zádori, Zoltán
Jefferson, Victoria A.
Csabai, Zsolt
Boldogkői, Miklós
Tombácz, Dóra
Meyer, Florencia
Boldogkői, Zsolt
author_sort Moldován, Norbert
collection PubMed
description Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.
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spelling pubmed-76863692020-11-27 Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing Moldován, Norbert Torma, Gábor Gulyás, Gábor Hornyák, Ákos Zádori, Zoltán Jefferson, Victoria A. Csabai, Zsolt Boldogkői, Miklós Tombácz, Dóra Meyer, Florencia Boldogkői, Zsolt Sci Rep Article Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps. Nature Publishing Group UK 2020-11-24 /pmc/articles/PMC7686369/ /pubmed/33235226 http://dx.doi.org/10.1038/s41598-020-77520-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moldován, Norbert
Torma, Gábor
Gulyás, Gábor
Hornyák, Ákos
Zádori, Zoltán
Jefferson, Victoria A.
Csabai, Zsolt
Boldogkői, Miklós
Tombácz, Dóra
Meyer, Florencia
Boldogkői, Zsolt
Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_full Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_fullStr Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_full_unstemmed Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_short Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_sort time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686369/
https://www.ncbi.nlm.nih.gov/pubmed/33235226
http://dx.doi.org/10.1038/s41598-020-77520-1
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