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Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study
OBJECTIVES: Early-onset myocardial infarction constitutes nearly one third of cases of myocardial infarction among Iraqis, which is rather higher than the proportions reported in many Western countries. Thus this study was initiated to investigate the role of some genetic polymorphisms, as well as a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686715/ https://www.ncbi.nlm.nih.gov/pubmed/33228791 http://dx.doi.org/10.1186/s13104-020-05367-w |
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author | Mohammad, Ameen M. Othman, Galawezh O. Saeed, Chiman H. Al Allawi, Sarah Gedeon, George S. Qadir, Shatha M. Al-Allawi, Nasir |
author_facet | Mohammad, Ameen M. Othman, Galawezh O. Saeed, Chiman H. Al Allawi, Sarah Gedeon, George S. Qadir, Shatha M. Al-Allawi, Nasir |
author_sort | Mohammad, Ameen M. |
collection | PubMed |
description | OBJECTIVES: Early-onset myocardial infarction constitutes nearly one third of cases of myocardial infarction among Iraqis, which is rather higher than the proportions reported in many Western countries. Thus this study was initiated to investigate the role of some genetic polymorphisms, as well as acquired risk factors in this condition. RESULTS: A total of 102 Iraqi patients with first myocardial infarction aged 50 years, and 77 matched controls were enrolled. The DNAs of participants were screened for nine polymorphisms, namely: β-Fibrinogen (− 455G > A), Factor XIII (V34L), Plasminogen Activator inhibitor-1 (PAI-1, 4G/5G), Human Platelet Antigen-1 (HPA1a/b), 5,10-Methylenetetrahydrofolate Reductase MTHFR (C677T) and MTHFR (A1298C), Angiotensin-Converting Enzyme (ACE) 287 bp insertion/deletion (I/D), Apolipoprotein-B (ApoB: R3500Q), and Apolipoprotein-E (Apo E: E2/E3/E4), using PCR and reverse hybridization technique. Among traditional risk factors, univariate analysis revealed that smoking (OR 2.86 [95%CI 1.53–5.34]), hyperlipidemia (OR 5.23 [95%CI 2.66–10.29]), and diabetes mellitus (OR 4.05 [95% CI 1.57–10.41]) were significantly higher among patients compared to controls (P<0.001, <0.001 and 0.002 respectively), while none of the nine genetic polymorphisms reached significance. Multivariate Logistic regression, however, revealed that only smoking and hyperlipidemia retained significance (P of < 0.001 each). The need to initiate further studies on larger cohorts is paramount to understand the higher than expected frequency of early-onset myocardial infarction in our population. |
format | Online Article Text |
id | pubmed-7686715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-76867152020-11-25 Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study Mohammad, Ameen M. Othman, Galawezh O. Saeed, Chiman H. Al Allawi, Sarah Gedeon, George S. Qadir, Shatha M. Al-Allawi, Nasir BMC Res Notes Research Note OBJECTIVES: Early-onset myocardial infarction constitutes nearly one third of cases of myocardial infarction among Iraqis, which is rather higher than the proportions reported in many Western countries. Thus this study was initiated to investigate the role of some genetic polymorphisms, as well as acquired risk factors in this condition. RESULTS: A total of 102 Iraqi patients with first myocardial infarction aged 50 years, and 77 matched controls were enrolled. The DNAs of participants were screened for nine polymorphisms, namely: β-Fibrinogen (− 455G > A), Factor XIII (V34L), Plasminogen Activator inhibitor-1 (PAI-1, 4G/5G), Human Platelet Antigen-1 (HPA1a/b), 5,10-Methylenetetrahydrofolate Reductase MTHFR (C677T) and MTHFR (A1298C), Angiotensin-Converting Enzyme (ACE) 287 bp insertion/deletion (I/D), Apolipoprotein-B (ApoB: R3500Q), and Apolipoprotein-E (Apo E: E2/E3/E4), using PCR and reverse hybridization technique. Among traditional risk factors, univariate analysis revealed that smoking (OR 2.86 [95%CI 1.53–5.34]), hyperlipidemia (OR 5.23 [95%CI 2.66–10.29]), and diabetes mellitus (OR 4.05 [95% CI 1.57–10.41]) were significantly higher among patients compared to controls (P<0.001, <0.001 and 0.002 respectively), while none of the nine genetic polymorphisms reached significance. Multivariate Logistic regression, however, revealed that only smoking and hyperlipidemia retained significance (P of < 0.001 each). The need to initiate further studies on larger cohorts is paramount to understand the higher than expected frequency of early-onset myocardial infarction in our population. BioMed Central 2020-11-24 /pmc/articles/PMC7686715/ /pubmed/33228791 http://dx.doi.org/10.1186/s13104-020-05367-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Mohammad, Ameen M. Othman, Galawezh O. Saeed, Chiman H. Al Allawi, Sarah Gedeon, George S. Qadir, Shatha M. Al-Allawi, Nasir Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study |
title | Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study |
title_full | Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study |
title_fullStr | Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study |
title_full_unstemmed | Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study |
title_short | Genetic polymorphisms in early-onset myocardial infarction in a sample of Iraqi patients: a pilot study |
title_sort | genetic polymorphisms in early-onset myocardial infarction in a sample of iraqi patients: a pilot study |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686715/ https://www.ncbi.nlm.nih.gov/pubmed/33228791 http://dx.doi.org/10.1186/s13104-020-05367-w |
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