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Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs

Transfer RNA‐derived small RNAs (tsRNAs), a novel type of non‐coding RNA derivative, are able to regulate a wide range of biological processes. What role these tsRNAs play in the regulation of human bone marrow mesenchymal stem cell (hMSCs) adipogenic differentiation remains uncertain. We induced th...

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Autores principales: Wang, Tao, Cao, Lingling, He, Shan, Long, Kai, Wang, Xinping, Yu, Hui, Ma, Baicheng, Xu, Xiaoyuan, Li, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686998/
https://www.ncbi.nlm.nih.gov/pubmed/32939933
http://dx.doi.org/10.1111/jcmm.15858
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author Wang, Tao
Cao, Lingling
He, Shan
Long, Kai
Wang, Xinping
Yu, Hui
Ma, Baicheng
Xu, Xiaoyuan
Li, Weidong
author_facet Wang, Tao
Cao, Lingling
He, Shan
Long, Kai
Wang, Xinping
Yu, Hui
Ma, Baicheng
Xu, Xiaoyuan
Li, Weidong
author_sort Wang, Tao
collection PubMed
description Transfer RNA‐derived small RNAs (tsRNAs), a novel type of non‐coding RNA derivative, are able to regulate a wide range of biological processes. What role these tsRNAs play in the regulation of human bone marrow mesenchymal stem cell (hMSCs) adipogenic differentiation remains uncertain. We induced the adipogenic differentiation of human bone marrow mesenchymal cells (hMSCs) and then performed small RNA transcriptomic sequencing, leading us to identify tsRNA‐06018 as a target of interest based upon resultant the tsRNA expression profiles. When tsRNA‐06018 was knocked down, this led to the inhibition of adipogenesis and a decrease in adipogenic marker expression. When STC2 was overexpressed, this impaired the adipogenic differentiation of these cells. We further used luciferase reporter assays to confirm that tsRNA‐06018 directly binds the 3′‐untranslated region (3′‐UTR) of STC2. In addition, we determined that both knocking down tsRNA‐06018 and overexpressing STC2 increased extracellular signal‐regulated kinase 1/2 (ERK1/2) phosphorylation within cells. We also assessed that the adipogenic differentiation of hMSCs in which tsRNA‐06018 was knocked down was further enhanced upon the addition of the ERK1/2 inhibitor U0126 as compared tsRNA‐06018 knockdown alone. Taken together, using small RNA sequencing we profiled tsRNAs in hMSCs during the process of adipogenesis, leading us to identify tsRNA‐06018 as a novel regulator of this differentiation process. This tsRNA was able to regulate adipogenic differentiation by targeting STC2 via the ERK1/2 signalling pathway.
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spelling pubmed-76869982020-12-03 Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs Wang, Tao Cao, Lingling He, Shan Long, Kai Wang, Xinping Yu, Hui Ma, Baicheng Xu, Xiaoyuan Li, Weidong J Cell Mol Med Original Articles Transfer RNA‐derived small RNAs (tsRNAs), a novel type of non‐coding RNA derivative, are able to regulate a wide range of biological processes. What role these tsRNAs play in the regulation of human bone marrow mesenchymal stem cell (hMSCs) adipogenic differentiation remains uncertain. We induced the adipogenic differentiation of human bone marrow mesenchymal cells (hMSCs) and then performed small RNA transcriptomic sequencing, leading us to identify tsRNA‐06018 as a target of interest based upon resultant the tsRNA expression profiles. When tsRNA‐06018 was knocked down, this led to the inhibition of adipogenesis and a decrease in adipogenic marker expression. When STC2 was overexpressed, this impaired the adipogenic differentiation of these cells. We further used luciferase reporter assays to confirm that tsRNA‐06018 directly binds the 3′‐untranslated region (3′‐UTR) of STC2. In addition, we determined that both knocking down tsRNA‐06018 and overexpressing STC2 increased extracellular signal‐regulated kinase 1/2 (ERK1/2) phosphorylation within cells. We also assessed that the adipogenic differentiation of hMSCs in which tsRNA‐06018 was knocked down was further enhanced upon the addition of the ERK1/2 inhibitor U0126 as compared tsRNA‐06018 knockdown alone. Taken together, using small RNA sequencing we profiled tsRNAs in hMSCs during the process of adipogenesis, leading us to identify tsRNA‐06018 as a novel regulator of this differentiation process. This tsRNA was able to regulate adipogenic differentiation by targeting STC2 via the ERK1/2 signalling pathway. John Wiley and Sons Inc. 2020-09-16 2020-11 /pmc/articles/PMC7686998/ /pubmed/32939933 http://dx.doi.org/10.1111/jcmm.15858 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Tao
Cao, Lingling
He, Shan
Long, Kai
Wang, Xinping
Yu, Hui
Ma, Baicheng
Xu, Xiaoyuan
Li, Weidong
Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs
title Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs
title_full Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs
title_fullStr Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs
title_full_unstemmed Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs
title_short Small RNA sequencing reveals a novel tsRNA‐06018 playing an important role during adipogenic differentiation of hMSCs
title_sort small rna sequencing reveals a novel tsrna‐06018 playing an important role during adipogenic differentiation of hmscs
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686998/
https://www.ncbi.nlm.nih.gov/pubmed/32939933
http://dx.doi.org/10.1111/jcmm.15858
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