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Identification and characterisation of the Volvox carteri Moco carrier protein
The molybdenum cofactor (Moco) is a redox active prosthetic group found in the active site of Moco-dependent enzymes (Mo-enzymes). As Moco and its intermediates are highly sensitive towards oxidative damage, these are believed to be permanently protein bound during synthesis and upon maturation. As...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687042/ https://www.ncbi.nlm.nih.gov/pubmed/33084886 http://dx.doi.org/10.1042/BSR20202351 |
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author | Hercher, Thomas W. Krausze, Joern Yang, Jing Kirk, Martin L. Kruse, Tobias |
author_facet | Hercher, Thomas W. Krausze, Joern Yang, Jing Kirk, Martin L. Kruse, Tobias |
author_sort | Hercher, Thomas W. |
collection | PubMed |
description | The molybdenum cofactor (Moco) is a redox active prosthetic group found in the active site of Moco-dependent enzymes (Mo-enzymes). As Moco and its intermediates are highly sensitive towards oxidative damage, these are believed to be permanently protein bound during synthesis and upon maturation. As a major component of the plant Moco transfer and storage system, proteins have been identified that are capable of Moco binding and release but do not possess Moco-dependent enzymatic activities. The first protein found to possess these properties was the Moco carrier protein (MCP) from the green alga Chlamydomonas reinhardtii. Here, we describe the identification and biochemical characterisation of the Volvox carteri (V. carteri) MCP and, for the first time, employ a comparative analysis to elucidate the principles behind MCP Moco binding. Doing so identified a sequence region of low homology amongst the existing MCPs, which we showed to be essential for Moco binding to V. carteri MCP. |
format | Online Article Text |
id | pubmed-7687042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76870422020-12-04 Identification and characterisation of the Volvox carteri Moco carrier protein Hercher, Thomas W. Krausze, Joern Yang, Jing Kirk, Martin L. Kruse, Tobias Biosci Rep Chemical Biology The molybdenum cofactor (Moco) is a redox active prosthetic group found in the active site of Moco-dependent enzymes (Mo-enzymes). As Moco and its intermediates are highly sensitive towards oxidative damage, these are believed to be permanently protein bound during synthesis and upon maturation. As a major component of the plant Moco transfer and storage system, proteins have been identified that are capable of Moco binding and release but do not possess Moco-dependent enzymatic activities. The first protein found to possess these properties was the Moco carrier protein (MCP) from the green alga Chlamydomonas reinhardtii. Here, we describe the identification and biochemical characterisation of the Volvox carteri (V. carteri) MCP and, for the first time, employ a comparative analysis to elucidate the principles behind MCP Moco binding. Doing so identified a sequence region of low homology amongst the existing MCPs, which we showed to be essential for Moco binding to V. carteri MCP. Portland Press Ltd. 2020-11-24 /pmc/articles/PMC7687042/ /pubmed/33084886 http://dx.doi.org/10.1042/BSR20202351 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the . |
spellingShingle | Chemical Biology Hercher, Thomas W. Krausze, Joern Yang, Jing Kirk, Martin L. Kruse, Tobias Identification and characterisation of the Volvox carteri Moco carrier protein |
title | Identification and characterisation of the Volvox carteri Moco carrier protein |
title_full | Identification and characterisation of the Volvox carteri Moco carrier protein |
title_fullStr | Identification and characterisation of the Volvox carteri Moco carrier protein |
title_full_unstemmed | Identification and characterisation of the Volvox carteri Moco carrier protein |
title_short | Identification and characterisation of the Volvox carteri Moco carrier protein |
title_sort | identification and characterisation of the volvox carteri moco carrier protein |
topic | Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687042/ https://www.ncbi.nlm.nih.gov/pubmed/33084886 http://dx.doi.org/10.1042/BSR20202351 |
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