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Identification and characterisation of the Volvox carteri Moco carrier protein

The molybdenum cofactor (Moco) is a redox active prosthetic group found in the active site of Moco-dependent enzymes (Mo-enzymes). As Moco and its intermediates are highly sensitive towards oxidative damage, these are believed to be permanently protein bound during synthesis and upon maturation. As...

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Autores principales: Hercher, Thomas W., Krausze, Joern, Yang, Jing, Kirk, Martin L., Kruse, Tobias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687042/
https://www.ncbi.nlm.nih.gov/pubmed/33084886
http://dx.doi.org/10.1042/BSR20202351
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author Hercher, Thomas W.
Krausze, Joern
Yang, Jing
Kirk, Martin L.
Kruse, Tobias
author_facet Hercher, Thomas W.
Krausze, Joern
Yang, Jing
Kirk, Martin L.
Kruse, Tobias
author_sort Hercher, Thomas W.
collection PubMed
description The molybdenum cofactor (Moco) is a redox active prosthetic group found in the active site of Moco-dependent enzymes (Mo-enzymes). As Moco and its intermediates are highly sensitive towards oxidative damage, these are believed to be permanently protein bound during synthesis and upon maturation. As a major component of the plant Moco transfer and storage system, proteins have been identified that are capable of Moco binding and release but do not possess Moco-dependent enzymatic activities. The first protein found to possess these properties was the Moco carrier protein (MCP) from the green alga Chlamydomonas reinhardtii. Here, we describe the identification and biochemical characterisation of the Volvox carteri (V. carteri) MCP and, for the first time, employ a comparative analysis to elucidate the principles behind MCP Moco binding. Doing so identified a sequence region of low homology amongst the existing MCPs, which we showed to be essential for Moco binding to V. carteri MCP.
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spelling pubmed-76870422020-12-04 Identification and characterisation of the Volvox carteri Moco carrier protein Hercher, Thomas W. Krausze, Joern Yang, Jing Kirk, Martin L. Kruse, Tobias Biosci Rep Chemical Biology The molybdenum cofactor (Moco) is a redox active prosthetic group found in the active site of Moco-dependent enzymes (Mo-enzymes). As Moco and its intermediates are highly sensitive towards oxidative damage, these are believed to be permanently protein bound during synthesis and upon maturation. As a major component of the plant Moco transfer and storage system, proteins have been identified that are capable of Moco binding and release but do not possess Moco-dependent enzymatic activities. The first protein found to possess these properties was the Moco carrier protein (MCP) from the green alga Chlamydomonas reinhardtii. Here, we describe the identification and biochemical characterisation of the Volvox carteri (V. carteri) MCP and, for the first time, employ a comparative analysis to elucidate the principles behind MCP Moco binding. Doing so identified a sequence region of low homology amongst the existing MCPs, which we showed to be essential for Moco binding to V. carteri MCP. Portland Press Ltd. 2020-11-24 /pmc/articles/PMC7687042/ /pubmed/33084886 http://dx.doi.org/10.1042/BSR20202351 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the .
spellingShingle Chemical Biology
Hercher, Thomas W.
Krausze, Joern
Yang, Jing
Kirk, Martin L.
Kruse, Tobias
Identification and characterisation of the Volvox carteri Moco carrier protein
title Identification and characterisation of the Volvox carteri Moco carrier protein
title_full Identification and characterisation of the Volvox carteri Moco carrier protein
title_fullStr Identification and characterisation of the Volvox carteri Moco carrier protein
title_full_unstemmed Identification and characterisation of the Volvox carteri Moco carrier protein
title_short Identification and characterisation of the Volvox carteri Moco carrier protein
title_sort identification and characterisation of the volvox carteri moco carrier protein
topic Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687042/
https://www.ncbi.nlm.nih.gov/pubmed/33084886
http://dx.doi.org/10.1042/BSR20202351
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