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Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand

Embalming fixatives such as formaldehyde and phenol have been associated with occupational health hazards. While anatomists aim at replacing these chemicals, this seems presently unfeasible in particular for formaldehyde. Furthermore, fixation protocols usually require well‐equipped facilities with...

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Autores principales: Crosado, Brynley, Löffler, Sabine, Ondruschka, Benjamin, Zhang, Ming, Zwirner, Johann, Hammer, Niels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687111/
https://www.ncbi.nlm.nih.gov/pubmed/31758863
http://dx.doi.org/10.1002/ase.1933
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author Crosado, Brynley
Löffler, Sabine
Ondruschka, Benjamin
Zhang, Ming
Zwirner, Johann
Hammer, Niels
author_facet Crosado, Brynley
Löffler, Sabine
Ondruschka, Benjamin
Zhang, Ming
Zwirner, Johann
Hammer, Niels
author_sort Crosado, Brynley
collection PubMed
description Embalming fixatives such as formaldehyde and phenol have been associated with occupational health hazards. While anatomists aim at replacing these chemicals, this seems presently unfeasible in particular for formaldehyde. Furthermore, fixation protocols usually require well‐equipped facilities with highly experienced staff to achieve good fixation results in spite of only a minimal use of formaldehyde. Combining these aspects, a technique robust enough to be carried out by morticians is presented, resulting in durable tissues with minimal formaldehyde use. An embalming protocol involving phenoxyethanol was established, using concentrations of 7 and 1.5 Vol% of phenoxyethanol in the fixative and the conservation fluid, respectively. Visual, haptic, histological, and biomechanical properties and their perceived potential to positively influence student learning outcomes were compared to standard embalming techniques. The phenoxyethanol technique provides esthetic, durable, and odorless tissues. Bleaching is less pronounced compared to ethanol‐ or formaldehyde‐based protocols. The tissues remain pliable following the phenoxyethanol‐based embalming and can be used for biomechanical experiments to some extent. Phenoxyethanol‐fixed tissues are well suited for undergraduate teaching with perceived positive learning outcomes and partly for postgraduate training. Phenoxyethanol tissues provide the option to obtain well‐preserved histology samples, similar to those derived from formaldehyde. The provided protocol helps replace the use of phenol and formaldehyde for conservation purposes and minimizes the use of formaldehyde for the initial injection fixation. Phenoxyethanol‐based embalming forms an effective alternative to standard embalming techniques for human cadavers. It is simple to use, allowing fixation procedures to be carried out in less sophisticated facilities with non‐anatomy staff.
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spelling pubmed-76871112020-12-03 Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand Crosado, Brynley Löffler, Sabine Ondruschka, Benjamin Zhang, Ming Zwirner, Johann Hammer, Niels Anat Sci Educ Regular Articles Embalming fixatives such as formaldehyde and phenol have been associated with occupational health hazards. While anatomists aim at replacing these chemicals, this seems presently unfeasible in particular for formaldehyde. Furthermore, fixation protocols usually require well‐equipped facilities with highly experienced staff to achieve good fixation results in spite of only a minimal use of formaldehyde. Combining these aspects, a technique robust enough to be carried out by morticians is presented, resulting in durable tissues with minimal formaldehyde use. An embalming protocol involving phenoxyethanol was established, using concentrations of 7 and 1.5 Vol% of phenoxyethanol in the fixative and the conservation fluid, respectively. Visual, haptic, histological, and biomechanical properties and their perceived potential to positively influence student learning outcomes were compared to standard embalming techniques. The phenoxyethanol technique provides esthetic, durable, and odorless tissues. Bleaching is less pronounced compared to ethanol‐ or formaldehyde‐based protocols. The tissues remain pliable following the phenoxyethanol‐based embalming and can be used for biomechanical experiments to some extent. Phenoxyethanol‐fixed tissues are well suited for undergraduate teaching with perceived positive learning outcomes and partly for postgraduate training. Phenoxyethanol tissues provide the option to obtain well‐preserved histology samples, similar to those derived from formaldehyde. The provided protocol helps replace the use of phenol and formaldehyde for conservation purposes and minimizes the use of formaldehyde for the initial injection fixation. Phenoxyethanol‐based embalming forms an effective alternative to standard embalming techniques for human cadavers. It is simple to use, allowing fixation procedures to be carried out in less sophisticated facilities with non‐anatomy staff. John Wiley and Sons Inc. 2020-01-21 2020 /pmc/articles/PMC7687111/ /pubmed/31758863 http://dx.doi.org/10.1002/ase.1933 Text en © 2019 The Authors. Anatomical Sciences Education published by Wiley Periodicals, Inc. on behalf of American Association of Anatomy This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Regular Articles
Crosado, Brynley
Löffler, Sabine
Ondruschka, Benjamin
Zhang, Ming
Zwirner, Johann
Hammer, Niels
Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand
title Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand
title_full Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand
title_fullStr Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand
title_full_unstemmed Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand
title_short Phenoxyethanol‐Based Embalming for Anatomy Teaching: An 18 Years' Experience with Crosado Embalming at the University of Otago in New Zealand
title_sort phenoxyethanol‐based embalming for anatomy teaching: an 18 years' experience with crosado embalming at the university of otago in new zealand
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687111/
https://www.ncbi.nlm.nih.gov/pubmed/31758863
http://dx.doi.org/10.1002/ase.1933
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