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Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization

Photonics based pre-clinical imaging is an extensively used technique to allow for the study of biologically relevant activity typically within a small-mouse model. Namely, bioluminescent tomography (BLT) attempts to tomographically reconstruct the 3-dimensional spatial light distribution of luminop...

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Detalles Bibliográficos
Autores principales: Bentley, Alexander, Rowe, Jonathan E., Dehghani, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687966/
https://www.ncbi.nlm.nih.gov/pubmed/33282499
http://dx.doi.org/10.1364/BOE.401671
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author Bentley, Alexander
Rowe, Jonathan E.
Dehghani, Hamid
author_facet Bentley, Alexander
Rowe, Jonathan E.
Dehghani, Hamid
author_sort Bentley, Alexander
collection PubMed
description Photonics based pre-clinical imaging is an extensively used technique to allow for the study of biologically relevant activity typically within a small-mouse model. Namely, bioluminescent tomography (BLT) attempts to tomographically reconstruct the 3-dimensional spatial light distribution of luminophores within a small animal given surface light measurements and known underlying optical parameters. Often it is the case where these optical parameters are unknown leading to the use of a ‘best’ guess approach or to direct measurements using either a multi-modal or dedicated system. Using these conventional approaches can lead to both inaccurate results and extending periods of imaging time. This work introduces the development of an algorithm that is used to accurately localize the spatial light distribution from a bioluminescence source within a subject by simultaneously reconstructing both the underlying optical properties and source spatial distribution and intensity from the same set of surface measurements. Through its application in 2- and 3-dimensional, homogeneous and heterogenous numerical models, it is demonstrated that the proposed algorithm is capable of replicating results as compared to ‘gold’ standard where the absolute optical properties are known. Additionally, the algorithm has been applied to experimental data using a tissue mimicking block phantom, recovering a spatial light distribution that has a localization error of ∼1.53 mm, which is better than previously published results without the need of assumptions regarding the underlying optical properties or source distribution.
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spelling pubmed-76879662020-12-03 Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization Bentley, Alexander Rowe, Jonathan E. Dehghani, Hamid Biomed Opt Express Article Photonics based pre-clinical imaging is an extensively used technique to allow for the study of biologically relevant activity typically within a small-mouse model. Namely, bioluminescent tomography (BLT) attempts to tomographically reconstruct the 3-dimensional spatial light distribution of luminophores within a small animal given surface light measurements and known underlying optical parameters. Often it is the case where these optical parameters are unknown leading to the use of a ‘best’ guess approach or to direct measurements using either a multi-modal or dedicated system. Using these conventional approaches can lead to both inaccurate results and extending periods of imaging time. This work introduces the development of an algorithm that is used to accurately localize the spatial light distribution from a bioluminescence source within a subject by simultaneously reconstructing both the underlying optical properties and source spatial distribution and intensity from the same set of surface measurements. Through its application in 2- and 3-dimensional, homogeneous and heterogenous numerical models, it is demonstrated that the proposed algorithm is capable of replicating results as compared to ‘gold’ standard where the absolute optical properties are known. Additionally, the algorithm has been applied to experimental data using a tissue mimicking block phantom, recovering a spatial light distribution that has a localization error of ∼1.53 mm, which is better than previously published results without the need of assumptions regarding the underlying optical properties or source distribution. Optical Society of America 2020-10-16 /pmc/articles/PMC7687966/ /pubmed/33282499 http://dx.doi.org/10.1364/BOE.401671 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
spellingShingle Article
Bentley, Alexander
Rowe, Jonathan E.
Dehghani, Hamid
Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization
title Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization
title_full Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization
title_fullStr Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization
title_full_unstemmed Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization
title_short Simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization
title_sort simultaneous diffuse optical and bioluminescence tomography to account for signal attenuation to improve source localization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687966/
https://www.ncbi.nlm.nih.gov/pubmed/33282499
http://dx.doi.org/10.1364/BOE.401671
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