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Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells

There has been an increase in the usage of heat-not-burn (HNB) cigarette products. However, their effects on alveolar epithelial cells (AECs) remain unknown. AECs are the target cells of conventional cigarette smoking-related respiratory diseases such as chronic obstructive pulmonary disease, idiopa...

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Autores principales: Ito, Yoko, Oshinden, Kana, Kutsuzawa, Naokata, Kohno, Chinatsu, Isaki, Sanae, Yokoyama, Keiko, Sato, Tadayuki, Tanaka, Masayuki, Asano, Koichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688177/
https://www.ncbi.nlm.nih.gov/pubmed/33237957
http://dx.doi.org/10.1371/journal.pone.0242789
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author Ito, Yoko
Oshinden, Kana
Kutsuzawa, Naokata
Kohno, Chinatsu
Isaki, Sanae
Yokoyama, Keiko
Sato, Tadayuki
Tanaka, Masayuki
Asano, Koichiro
author_facet Ito, Yoko
Oshinden, Kana
Kutsuzawa, Naokata
Kohno, Chinatsu
Isaki, Sanae
Yokoyama, Keiko
Sato, Tadayuki
Tanaka, Masayuki
Asano, Koichiro
author_sort Ito, Yoko
collection PubMed
description There has been an increase in the usage of heat-not-burn (HNB) cigarette products. However, their effects on alveolar epithelial cells (AECs) remain unknown. AECs are the target cells of conventional cigarette smoking-related respiratory diseases such as chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis and lung cancer whose pathogenesis involves oxidative stress. In this study, primary rat AECs were isolated, cultured and stimulated by HNB cigarette smoke extract (CSE). Our data indicate that rat AECs exposed to HNB CSE induced oxidative stress response genes (e.g. Hmox-1, Gsta1, Gsta3 and Nqo1). We also compared the oxidative stress response between two different types of AECs, alveolar type I-like (ATI-like) cells and type II (ATII) cells, and between two different types of cigarette, HNB cigarettes and conventional cigarettes. The expressions of Gsta1, Gsta3 and Nqo1 were higher in ATII cells than ATI-like cells in response to HNB and conventional cigarettes, but there was no significant difference in their expression levels between HNB cigarette and conventional cigarette. Taken together, our results suggest that HNB cigarettes have the similar potential as conventional cigarette products to induce oxidative stress response in AECs.
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spelling pubmed-76881772020-12-05 Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells Ito, Yoko Oshinden, Kana Kutsuzawa, Naokata Kohno, Chinatsu Isaki, Sanae Yokoyama, Keiko Sato, Tadayuki Tanaka, Masayuki Asano, Koichiro PLoS One Research Article There has been an increase in the usage of heat-not-burn (HNB) cigarette products. However, their effects on alveolar epithelial cells (AECs) remain unknown. AECs are the target cells of conventional cigarette smoking-related respiratory diseases such as chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis and lung cancer whose pathogenesis involves oxidative stress. In this study, primary rat AECs were isolated, cultured and stimulated by HNB cigarette smoke extract (CSE). Our data indicate that rat AECs exposed to HNB CSE induced oxidative stress response genes (e.g. Hmox-1, Gsta1, Gsta3 and Nqo1). We also compared the oxidative stress response between two different types of AECs, alveolar type I-like (ATI-like) cells and type II (ATII) cells, and between two different types of cigarette, HNB cigarettes and conventional cigarettes. The expressions of Gsta1, Gsta3 and Nqo1 were higher in ATII cells than ATI-like cells in response to HNB and conventional cigarettes, but there was no significant difference in their expression levels between HNB cigarette and conventional cigarette. Taken together, our results suggest that HNB cigarettes have the similar potential as conventional cigarette products to induce oxidative stress response in AECs. Public Library of Science 2020-11-25 /pmc/articles/PMC7688177/ /pubmed/33237957 http://dx.doi.org/10.1371/journal.pone.0242789 Text en © 2020 Ito et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ito, Yoko
Oshinden, Kana
Kutsuzawa, Naokata
Kohno, Chinatsu
Isaki, Sanae
Yokoyama, Keiko
Sato, Tadayuki
Tanaka, Masayuki
Asano, Koichiro
Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells
title Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells
title_full Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells
title_fullStr Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells
title_full_unstemmed Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells
title_short Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells
title_sort heat-not-burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688177/
https://www.ncbi.nlm.nih.gov/pubmed/33237957
http://dx.doi.org/10.1371/journal.pone.0242789
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