Label-free X-ray estimation of brain amyloid burden

Amyloid plaque deposits in the brain are indicative of Alzheimer’s and other diseases. Measurements of brain amyloid burden in small animals require laborious post-mortem histological analysis or resource-intensive, contrast-enhanced imaging techniques. We describe a label-free method based on spect...

Descripción completa

Detalles Bibliográficos
Autores principales: Dahal, Eshan, Ghammraoui, Bahaa, Ye, Meijun, Smith, J. Carson, Badano, Aldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689528/
https://www.ncbi.nlm.nih.gov/pubmed/33239703
http://dx.doi.org/10.1038/s41598-020-77554-5
_version_ 1783613880105172992
author Dahal, Eshan
Ghammraoui, Bahaa
Ye, Meijun
Smith, J. Carson
Badano, Aldo
author_facet Dahal, Eshan
Ghammraoui, Bahaa
Ye, Meijun
Smith, J. Carson
Badano, Aldo
author_sort Dahal, Eshan
collection PubMed
description Amyloid plaque deposits in the brain are indicative of Alzheimer’s and other diseases. Measurements of brain amyloid burden in small animals require laborious post-mortem histological analysis or resource-intensive, contrast-enhanced imaging techniques. We describe a label-free method based on spectral small-angle X-ray scattering with a polychromatic beam for in vivo estimation of brain amyloid burden. Our findings comparing 5XFAD versus wild-type mice correlate well with histology, showing promise for a fast and practical in vivo technique.
format Online
Article
Text
id pubmed-7689528
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-76895282020-11-27 Label-free X-ray estimation of brain amyloid burden Dahal, Eshan Ghammraoui, Bahaa Ye, Meijun Smith, J. Carson Badano, Aldo Sci Rep Article Amyloid plaque deposits in the brain are indicative of Alzheimer’s and other diseases. Measurements of brain amyloid burden in small animals require laborious post-mortem histological analysis or resource-intensive, contrast-enhanced imaging techniques. We describe a label-free method based on spectral small-angle X-ray scattering with a polychromatic beam for in vivo estimation of brain amyloid burden. Our findings comparing 5XFAD versus wild-type mice correlate well with histology, showing promise for a fast and practical in vivo technique. Nature Publishing Group UK 2020-11-25 /pmc/articles/PMC7689528/ /pubmed/33239703 http://dx.doi.org/10.1038/s41598-020-77554-5 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dahal, Eshan
Ghammraoui, Bahaa
Ye, Meijun
Smith, J. Carson
Badano, Aldo
Label-free X-ray estimation of brain amyloid burden
title Label-free X-ray estimation of brain amyloid burden
title_full Label-free X-ray estimation of brain amyloid burden
title_fullStr Label-free X-ray estimation of brain amyloid burden
title_full_unstemmed Label-free X-ray estimation of brain amyloid burden
title_short Label-free X-ray estimation of brain amyloid burden
title_sort label-free x-ray estimation of brain amyloid burden
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689528/
https://www.ncbi.nlm.nih.gov/pubmed/33239703
http://dx.doi.org/10.1038/s41598-020-77554-5
work_keys_str_mv AT dahaleshan labelfreexrayestimationofbrainamyloidburden
AT ghammraouibahaa labelfreexrayestimationofbrainamyloidburden
AT yemeijun labelfreexrayestimationofbrainamyloidburden
AT smithjcarson labelfreexrayestimationofbrainamyloidburden
AT badanoaldo labelfreexrayestimationofbrainamyloidburden

Ejemplares similares