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Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China
The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 39...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689805/ https://www.ncbi.nlm.nih.gov/pubmed/32619331 http://dx.doi.org/10.1111/1755-0998.13219 |
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author | Zhang, Lei Liu, Bo Zheng, Weigang Liu, Conghui Zhang, Dandan Zhao, Shengyuan Li, Zaiyuan Xu, Pengjun Wilson, Kenneth Withers, Amy Jones, Christopher M. Smith, Judith A. Chipabika, Gilson Kachigamba, Donald L. Nam, Kiwoong d’Alençon, Emmanuelle Liu, Bei Liang, Xinyue Jin, Minghui Wu, Chao Chakrabarty, Swapan Yang, Xianming Jiang, Yuying Liu, Jie Liu, Xiaolin Quan, Weipeng Wang, Guirong Fan, Wei Qian, Wanqiang Wu, Kongming Xiao, Yutao |
author_facet | Zhang, Lei Liu, Bo Zheng, Weigang Liu, Conghui Zhang, Dandan Zhao, Shengyuan Li, Zaiyuan Xu, Pengjun Wilson, Kenneth Withers, Amy Jones, Christopher M. Smith, Judith A. Chipabika, Gilson Kachigamba, Donald L. Nam, Kiwoong d’Alençon, Emmanuelle Liu, Bei Liang, Xinyue Jin, Minghui Wu, Chao Chakrabarty, Swapan Yang, Xianming Jiang, Yuying Liu, Jie Liu, Xiaolin Quan, Weipeng Wang, Guirong Fan, Wei Qian, Wanqiang Wu, Kongming Xiao, Yutao |
author_sort | Zhang, Lei |
collection | PubMed |
description | The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38‐Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22,260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bacillus thuringiensis (Bt) resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bt toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm. |
format | Online Article Text |
id | pubmed-7689805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76898052020-12-05 Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China Zhang, Lei Liu, Bo Zheng, Weigang Liu, Conghui Zhang, Dandan Zhao, Shengyuan Li, Zaiyuan Xu, Pengjun Wilson, Kenneth Withers, Amy Jones, Christopher M. Smith, Judith A. Chipabika, Gilson Kachigamba, Donald L. Nam, Kiwoong d’Alençon, Emmanuelle Liu, Bei Liang, Xinyue Jin, Minghui Wu, Chao Chakrabarty, Swapan Yang, Xianming Jiang, Yuying Liu, Jie Liu, Xiaolin Quan, Weipeng Wang, Guirong Fan, Wei Qian, Wanqiang Wu, Kongming Xiao, Yutao Mol Ecol Resour RESOURCE ARTICLES The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38‐Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22,260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bacillus thuringiensis (Bt) resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bt toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm. John Wiley and Sons Inc. 2020-07-20 2020-11 /pmc/articles/PMC7689805/ /pubmed/32619331 http://dx.doi.org/10.1111/1755-0998.13219 Text en © The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RESOURCE ARTICLES Zhang, Lei Liu, Bo Zheng, Weigang Liu, Conghui Zhang, Dandan Zhao, Shengyuan Li, Zaiyuan Xu, Pengjun Wilson, Kenneth Withers, Amy Jones, Christopher M. Smith, Judith A. Chipabika, Gilson Kachigamba, Donald L. Nam, Kiwoong d’Alençon, Emmanuelle Liu, Bei Liang, Xinyue Jin, Minghui Wu, Chao Chakrabarty, Swapan Yang, Xianming Jiang, Yuying Liu, Jie Liu, Xiaolin Quan, Weipeng Wang, Guirong Fan, Wei Qian, Wanqiang Wu, Kongming Xiao, Yutao Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China |
title | Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China |
title_full | Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China |
title_fullStr | Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China |
title_full_unstemmed | Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China |
title_short | Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China |
title_sort | genetic structure and insecticide resistance characteristics of fall armyworm populations invading china |
topic | RESOURCE ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689805/ https://www.ncbi.nlm.nih.gov/pubmed/32619331 http://dx.doi.org/10.1111/1755-0998.13219 |
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