Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites

Herein, we report the targeted isolation and characterization of four linear nonribosomally synthesized tetrapeptides (pseudoxylaramide A–D) and two cyclic nonribosomal peptide synthetase‐polyketide synthase‐derived natural products (xylacremolide A and B) from the termite‐associated stowaway fungus...

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Autores principales: Schalk, Felix, Um, Soohyun, Guo, Huijuan, Kreuzenbeck, Nina B., Görls, Helmar, de Beer, Z. Wilhelm, Beemelmanns, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689812/
https://www.ncbi.nlm.nih.gov/pubmed/32470183
http://dx.doi.org/10.1002/cbic.202000331
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author Schalk, Felix
Um, Soohyun
Guo, Huijuan
Kreuzenbeck, Nina B.
Görls, Helmar
de Beer, Z. Wilhelm
Beemelmanns, Christine
author_facet Schalk, Felix
Um, Soohyun
Guo, Huijuan
Kreuzenbeck, Nina B.
Görls, Helmar
de Beer, Z. Wilhelm
Beemelmanns, Christine
author_sort Schalk, Felix
collection PubMed
description Herein, we report the targeted isolation and characterization of four linear nonribosomally synthesized tetrapeptides (pseudoxylaramide A–D) and two cyclic nonribosomal peptide synthetase‐polyketide synthase‐derived natural products (xylacremolide A and B) from the termite‐associated stowaway fungus Pseudoxylaria sp. X187. The fungal strain was prioritized for further metabolic analysis based on its taxonomical position and morphological and bioassay data. Metabolic data were dereplicated based on high‐resolution tandem mass spectrometry data and global molecular networking analysis. The structure of all six new natural products was elucidated based on a combination of 1D and 2D NMR analysis, Marfey's analysis and X‐ray crystallography.
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spelling pubmed-76898122020-12-08 Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites Schalk, Felix Um, Soohyun Guo, Huijuan Kreuzenbeck, Nina B. Görls, Helmar de Beer, Z. Wilhelm Beemelmanns, Christine Chembiochem Full Papers Herein, we report the targeted isolation and characterization of four linear nonribosomally synthesized tetrapeptides (pseudoxylaramide A–D) and two cyclic nonribosomal peptide synthetase‐polyketide synthase‐derived natural products (xylacremolide A and B) from the termite‐associated stowaway fungus Pseudoxylaria sp. X187. The fungal strain was prioritized for further metabolic analysis based on its taxonomical position and morphological and bioassay data. Metabolic data were dereplicated based on high‐resolution tandem mass spectrometry data and global molecular networking analysis. The structure of all six new natural products was elucidated based on a combination of 1D and 2D NMR analysis, Marfey's analysis and X‐ray crystallography. John Wiley and Sons Inc. 2020-07-02 2020-10-15 /pmc/articles/PMC7689812/ /pubmed/32470183 http://dx.doi.org/10.1002/cbic.202000331 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Full Papers
Schalk, Felix
Um, Soohyun
Guo, Huijuan
Kreuzenbeck, Nina B.
Görls, Helmar
de Beer, Z. Wilhelm
Beemelmanns, Christine
Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites
title Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites
title_full Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites
title_fullStr Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites
title_full_unstemmed Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites
title_short Targeted Discovery of Tetrapeptides and Cyclic Polyketide‐Peptide Hybrids from a Fungal Antagonist of Farming Termites
title_sort targeted discovery of tetrapeptides and cyclic polyketide‐peptide hybrids from a fungal antagonist of farming termites
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689812/
https://www.ncbi.nlm.nih.gov/pubmed/32470183
http://dx.doi.org/10.1002/cbic.202000331
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