Modular and Versatile Trans‐Encoded Genetic Switches

Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans‐encoded and based on tRNA‐mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be des...

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Detalles Bibliográficos
Autores principales: Paul, Avishek, Warszawik, Eliza M., Loznik, Mark, Boersma, Arnold J., Herrmann, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689881/
https://www.ncbi.nlm.nih.gov/pubmed/32352201
http://dx.doi.org/10.1002/anie.202001372
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author Paul, Avishek
Warszawik, Eliza M.
Loznik, Mark
Boersma, Arnold J.
Herrmann, Andreas
author_facet Paul, Avishek
Warszawik, Eliza M.
Loznik, Mark
Boersma, Arnold J.
Herrmann, Andreas
author_sort Paul, Avishek
collection PubMed
description Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans‐encoded and based on tRNA‐mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs, including RNA, small molecules, and proteins. This powerful approach enables control of the translation of protein expression from plasmid and genome DNA.
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spelling pubmed-76898812020-12-08 Modular and Versatile Trans‐Encoded Genetic Switches Paul, Avishek Warszawik, Eliza M. Loznik, Mark Boersma, Arnold J. Herrmann, Andreas Angew Chem Int Ed Engl Communications Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans‐encoded and based on tRNA‐mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs, including RNA, small molecules, and proteins. This powerful approach enables control of the translation of protein expression from plasmid and genome DNA. John Wiley and Sons Inc. 2020-07-27 2020-11-09 /pmc/articles/PMC7689881/ /pubmed/32352201 http://dx.doi.org/10.1002/anie.202001372 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Paul, Avishek
Warszawik, Eliza M.
Loznik, Mark
Boersma, Arnold J.
Herrmann, Andreas
Modular and Versatile Trans‐Encoded Genetic Switches
title Modular and Versatile Trans‐Encoded Genetic Switches
title_full Modular and Versatile Trans‐Encoded Genetic Switches
title_fullStr Modular and Versatile Trans‐Encoded Genetic Switches
title_full_unstemmed Modular and Versatile Trans‐Encoded Genetic Switches
title_short Modular and Versatile Trans‐Encoded Genetic Switches
title_sort modular and versatile trans‐encoded genetic switches
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689881/
https://www.ncbi.nlm.nih.gov/pubmed/32352201
http://dx.doi.org/10.1002/anie.202001372
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AT loznikmark modularandversatiletransencodedgeneticswitches
AT boersmaarnoldj modularandversatiletransencodedgeneticswitches
AT herrmannandreas modularandversatiletransencodedgeneticswitches

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