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PCR Multiplexing Based on a Single Fluorescent Channel Using Dynamic Melting Curve Analysis
[Image: see text] Since its invention in 1986, the polymerase chain reaction (PCR), has become a well-established method for the detection and amplification of deoxyribonucleic acid (DNA) with a specific sequence. Incorporating fluorescent probes, known as TaqMan probes, or DNA intercalating dyes, s...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689941/ https://www.ncbi.nlm.nih.gov/pubmed/33251461 http://dx.doi.org/10.1021/acsomega.0c04766 |