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Light-Activated Signaling in DNA-Encoded Sender–Receiver Architectures
[Image: see text] Collective decision making by living cells is facilitated by exchange of diffusible signals where sender cells release a chemical signal that is interpreted by receiver cells. A variety of nonliving artificial cell models have been developed in recent years that mimic various aspec...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690052/ https://www.ncbi.nlm.nih.gov/pubmed/33078948 http://dx.doi.org/10.1021/acsnano.0c07537 |
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author | Yang, Shuo Pieters, Pascal A. Joesaar, Alex Bögels, Bas W. A. Brouwers, Rens Myrgorodska, Iuliia Mann, Stephen de Greef, Tom F. A. |
author_facet | Yang, Shuo Pieters, Pascal A. Joesaar, Alex Bögels, Bas W. A. Brouwers, Rens Myrgorodska, Iuliia Mann, Stephen de Greef, Tom F. A. |
author_sort | Yang, Shuo |
collection | PubMed |
description | [Image: see text] Collective decision making by living cells is facilitated by exchange of diffusible signals where sender cells release a chemical signal that is interpreted by receiver cells. A variety of nonliving artificial cell models have been developed in recent years that mimic various aspects of diffusion-based intercellular communication. However, localized secretion of diffusive signals from individual protocells, which is critical for mimicking biological sender–receiver systems, has remained challenging to control precisely. Here, we engineer light-responsive, DNA-encoded sender–receiver architectures, where protein–polymer microcapsules act as cell mimics and molecular communication occurs through diffusive DNA signals. We prepare spatial distributions of sender and receiver protocells using a microfluidic trapping array and set up a signaling gradient from a single sender cell using light, which activates surrounding receivers through DNA strand displacement. Our systematic analysis reveals how the effective signal range of a single sender is determined by various factors including the density and permeability of receivers, extracellular signal degradation, signal consumption, and catalytic regeneration. In addition, we construct a three-population configuration where two sender cells are embedded in a dense array of receivers that implement Boolean logic and investigate spatial integration of nonidentical input cues. The results offer a means for studying diffusion-based sender–receiver topologies and present a strategy to achieve the congruence of reaction-diffusion and positional information in chemical communication systems that have the potential to reconstitute collective cellular patterns. |
format | Online Article Text |
id | pubmed-7690052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-76900522020-11-27 Light-Activated Signaling in DNA-Encoded Sender–Receiver Architectures Yang, Shuo Pieters, Pascal A. Joesaar, Alex Bögels, Bas W. A. Brouwers, Rens Myrgorodska, Iuliia Mann, Stephen de Greef, Tom F. A. ACS Nano [Image: see text] Collective decision making by living cells is facilitated by exchange of diffusible signals where sender cells release a chemical signal that is interpreted by receiver cells. A variety of nonliving artificial cell models have been developed in recent years that mimic various aspects of diffusion-based intercellular communication. However, localized secretion of diffusive signals from individual protocells, which is critical for mimicking biological sender–receiver systems, has remained challenging to control precisely. Here, we engineer light-responsive, DNA-encoded sender–receiver architectures, where protein–polymer microcapsules act as cell mimics and molecular communication occurs through diffusive DNA signals. We prepare spatial distributions of sender and receiver protocells using a microfluidic trapping array and set up a signaling gradient from a single sender cell using light, which activates surrounding receivers through DNA strand displacement. Our systematic analysis reveals how the effective signal range of a single sender is determined by various factors including the density and permeability of receivers, extracellular signal degradation, signal consumption, and catalytic regeneration. In addition, we construct a three-population configuration where two sender cells are embedded in a dense array of receivers that implement Boolean logic and investigate spatial integration of nonidentical input cues. The results offer a means for studying diffusion-based sender–receiver topologies and present a strategy to achieve the congruence of reaction-diffusion and positional information in chemical communication systems that have the potential to reconstitute collective cellular patterns. American Chemical Society 2020-10-20 2020-11-24 /pmc/articles/PMC7690052/ /pubmed/33078948 http://dx.doi.org/10.1021/acsnano.0c07537 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Yang, Shuo Pieters, Pascal A. Joesaar, Alex Bögels, Bas W. A. Brouwers, Rens Myrgorodska, Iuliia Mann, Stephen de Greef, Tom F. A. Light-Activated Signaling in DNA-Encoded Sender–Receiver Architectures |
title | Light-Activated
Signaling in DNA-Encoded Sender–Receiver
Architectures |
title_full | Light-Activated
Signaling in DNA-Encoded Sender–Receiver
Architectures |
title_fullStr | Light-Activated
Signaling in DNA-Encoded Sender–Receiver
Architectures |
title_full_unstemmed | Light-Activated
Signaling in DNA-Encoded Sender–Receiver
Architectures |
title_short | Light-Activated
Signaling in DNA-Encoded Sender–Receiver
Architectures |
title_sort | light-activated
signaling in dna-encoded sender–receiver
architectures |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690052/ https://www.ncbi.nlm.nih.gov/pubmed/33078948 http://dx.doi.org/10.1021/acsnano.0c07537 |
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