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Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study
In vivo monitoring of the microvasculature is relevant since diseases such as diabetes, ischemia, or cancer cause microvascular impairment. Super-resolution ultrasound imaging allows in vivo examination of the microvasculature by detecting and tracking sparsely distributed intravascular microbubbles...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690607/ https://www.ncbi.nlm.nih.gov/pubmed/33105888 http://dx.doi.org/10.3390/diagnostics10110862 |
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author | Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Søgaard, Stinne Byrholdt Gran, Fredrik Lönn, Lars Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin |
author_facet | Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Søgaard, Stinne Byrholdt Gran, Fredrik Lönn, Lars Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin |
author_sort | Andersen, Sofie Bech |
collection | PubMed |
description | In vivo monitoring of the microvasculature is relevant since diseases such as diabetes, ischemia, or cancer cause microvascular impairment. Super-resolution ultrasound imaging allows in vivo examination of the microvasculature by detecting and tracking sparsely distributed intravascular microbubbles over a minute-long period. The ability to create detailed images of the renal vasculature of Sprague-Dawley rats using a modified clinical ultrasound platform was investigated in this study. Additionally, we hypothesized that early ischemic damage to the renal microcirculation could be visualized. After a baseline scan of the exposed kidney, 10 rats underwent clamping of the renal vein (n = 5) or artery (n = 5) for 45 min. The kidneys were rescanned at the onset of clamp release and after 60 min of reperfusion. Using a processing pipeline for tissue motion compensation and microbubble tracking, super-resolution images with a very high level of detail were constructed. Image filtration allowed further characterization of the vasculature by isolating specific vessels such as the ascending vasa recta with a 15–20 μm diameter. Using the super-resolution images alone, it was only possible for six assessors to consistently distinguish the healthy renal microvasculature from the microvasculature at the onset of vein clamp release. Future studies will aim at attaining quantitative estimations of alterations in the renal microvascular blood flow using super-resolution ultrasound imaging. |
format | Online Article Text |
id | pubmed-7690607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76906072020-11-27 Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Søgaard, Stinne Byrholdt Gran, Fredrik Lönn, Lars Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin Diagnostics (Basel) Article In vivo monitoring of the microvasculature is relevant since diseases such as diabetes, ischemia, or cancer cause microvascular impairment. Super-resolution ultrasound imaging allows in vivo examination of the microvasculature by detecting and tracking sparsely distributed intravascular microbubbles over a minute-long period. The ability to create detailed images of the renal vasculature of Sprague-Dawley rats using a modified clinical ultrasound platform was investigated in this study. Additionally, we hypothesized that early ischemic damage to the renal microcirculation could be visualized. After a baseline scan of the exposed kidney, 10 rats underwent clamping of the renal vein (n = 5) or artery (n = 5) for 45 min. The kidneys were rescanned at the onset of clamp release and after 60 min of reperfusion. Using a processing pipeline for tissue motion compensation and microbubble tracking, super-resolution images with a very high level of detail were constructed. Image filtration allowed further characterization of the vasculature by isolating specific vessels such as the ascending vasa recta with a 15–20 μm diameter. Using the super-resolution images alone, it was only possible for six assessors to consistently distinguish the healthy renal microvasculature from the microvasculature at the onset of vein clamp release. Future studies will aim at attaining quantitative estimations of alterations in the renal microvascular blood flow using super-resolution ultrasound imaging. MDPI 2020-10-22 /pmc/articles/PMC7690607/ /pubmed/33105888 http://dx.doi.org/10.3390/diagnostics10110862 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Søgaard, Stinne Byrholdt Gran, Fredrik Lönn, Lars Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study |
title | Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study |
title_full | Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study |
title_fullStr | Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study |
title_full_unstemmed | Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study |
title_short | Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study |
title_sort | super-resolution imaging with ultrasound for visualization of the renal microvasculature in rats before and after renal ischemia: a pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690607/ https://www.ncbi.nlm.nih.gov/pubmed/33105888 http://dx.doi.org/10.3390/diagnostics10110862 |
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