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De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs
A number of studies have shown that glycosylation of proteins plays diverse functions in the lives of organisms, has crucial biological and physiological roles in pathogen–host interactions, and is involved in a large number of biological events in the immune system, and in virus and bacteria recogn...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690630/ https://www.ncbi.nlm.nih.gov/pubmed/33105875 http://dx.doi.org/10.3390/biom10111470 |
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author | Dolashka, Pavlina Daskalova, Asya Dolashki, Aleksandar Voelter, Wolfgang |
author_facet | Dolashka, Pavlina Daskalova, Asya Dolashki, Aleksandar Voelter, Wolfgang |
author_sort | Dolashka, Pavlina |
collection | PubMed |
description | A number of studies have shown that glycosylation of proteins plays diverse functions in the lives of organisms, has crucial biological and physiological roles in pathogen–host interactions, and is involved in a large number of biological events in the immune system, and in virus and bacteria recognition. The large amount of scientific interest in glycoproteins of molluscan hemocyanins is due not only to their complex quaternary structures, but also to the great diversity of their oligosaccharide structures with a high carbohydrate content (2–9%). This great variety is due to their specific monosaccharide composition and different side chain composition. The determination of glycans and glycopeptides was performed with the most commonly used methods for the analysis of biomolecules, including peptides and proteins, including Matrix Assisted Laser Desorption/Ionisation–Time of Flight (MALDI-TOF-TOF), Liquid Chromatography - Electrospray Ionization-Mass Spectrometry (LC/ESI-MS), Liquid Chromatography (LC-Q-trap-MS/MS) or Nano- Electrospray Ionization-Mass Spectrometry (nano-ESI-MS) and others. The molluscan hemocyanins have complex carbohydrate structures with predominant N-linked glycans. Of interest are identified structures with methylated hexoses and xyloses arranged at different positions in the carbohydrate moieties of molluscan hemocyanins. Novel acidic glycan structures with specific glycosylation positions, e.g., hemocyanins that enable a deeper insight into the glycosylation process, were observed in Rapana venosa, Helix lucorum, and Haliotis tuberculata. Recent studies demonstrate that glycosylation plays a crucial physiological role in the immunostimulatory and therapeutic effect of glycoproteins. The remarkable diversity of hemocyanin glycan content is an important feature of their immune function and provides a new concept in the antibody–antigen interaction through clustered carbohydrate epitopes. |
format | Online Article Text |
id | pubmed-7690630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76906302020-11-27 De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs Dolashka, Pavlina Daskalova, Asya Dolashki, Aleksandar Voelter, Wolfgang Biomolecules Review A number of studies have shown that glycosylation of proteins plays diverse functions in the lives of organisms, has crucial biological and physiological roles in pathogen–host interactions, and is involved in a large number of biological events in the immune system, and in virus and bacteria recognition. The large amount of scientific interest in glycoproteins of molluscan hemocyanins is due not only to their complex quaternary structures, but also to the great diversity of their oligosaccharide structures with a high carbohydrate content (2–9%). This great variety is due to their specific monosaccharide composition and different side chain composition. The determination of glycans and glycopeptides was performed with the most commonly used methods for the analysis of biomolecules, including peptides and proteins, including Matrix Assisted Laser Desorption/Ionisation–Time of Flight (MALDI-TOF-TOF), Liquid Chromatography - Electrospray Ionization-Mass Spectrometry (LC/ESI-MS), Liquid Chromatography (LC-Q-trap-MS/MS) or Nano- Electrospray Ionization-Mass Spectrometry (nano-ESI-MS) and others. The molluscan hemocyanins have complex carbohydrate structures with predominant N-linked glycans. Of interest are identified structures with methylated hexoses and xyloses arranged at different positions in the carbohydrate moieties of molluscan hemocyanins. Novel acidic glycan structures with specific glycosylation positions, e.g., hemocyanins that enable a deeper insight into the glycosylation process, were observed in Rapana venosa, Helix lucorum, and Haliotis tuberculata. Recent studies demonstrate that glycosylation plays a crucial physiological role in the immunostimulatory and therapeutic effect of glycoproteins. The remarkable diversity of hemocyanin glycan content is an important feature of their immune function and provides a new concept in the antibody–antigen interaction through clustered carbohydrate epitopes. MDPI 2020-10-22 /pmc/articles/PMC7690630/ /pubmed/33105875 http://dx.doi.org/10.3390/biom10111470 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Dolashka, Pavlina Daskalova, Asya Dolashki, Aleksandar Voelter, Wolfgang De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title | De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_full | De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_fullStr | De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_full_unstemmed | De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_short | De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_sort | de novo structural determination of the oligosaccharide structure of hemocyanins from molluscs |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690630/ https://www.ncbi.nlm.nih.gov/pubmed/33105875 http://dx.doi.org/10.3390/biom10111470 |
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